Abstract：
:Hepatic microsomal cytochrome P450, EROD and ECOD activity were investigated as biomarkers of PCB exposure in harbour seals (Phoca vitulina). Due to the difficulty of obtaining undegraded seal liver samples, standard spectrophotometric methodology was adapted to investigate P420 (degraded P450) as a PCB biomarker with partially degraded samples. Total PCB burdens in both blubber and liver had positive correlations with P450, P420 and MFO activity levels. The use of P420 biomarkers in this study supports the inclusion of samples from by-caught marine mammals for future biomonitoring studies. P450 isozymes CYP1A (P4501A) and CYP2B (P4502B) in conjunction with MFO activity were investigated as "specific" biomarkers of PCB exposure. They were found to reliably reflect levels of [MC] and [PB]-type PCB exposure in harbour seal liver.
journal_name
Chemospherejournal_title
Chemosphereauthors
Troisi GM,Mason CFdoi
10.1016/s0045-6535(97)00268-3subject
Has Abstractpub_date
1997-11-01 00:00:00pages
1933-46issue
9eissn
0045-6535issn
1879-1298pii
S0045653597002683journal_volume
35pub_type
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