Abstract：
PURPOSE:The antiproliferative and antisecretory effects of somatostatin have many potential uses in the clinical setting. Retroviral gene transfer of somatostatin to endothelium is a potential means of local delivery of this peptide to specific vascular beds. This investigation was designed to determine whether transduced endothelial cells (ECs) could produce and post-translationally process somatostatin. METHODS:Cultured canine venous, rat aortic, and rat microvascular ECs were transfected with retroviruses containing a human somatostatin cDNA or a control beta-galactosidase gene. Total and isoform somatostatin production and uniformity of beta-galactosidase expression were analyzed, as were the effects of somatostatin production on EC proliferation. RESULTS:Somatostatin-transduced canine venous ECs, but not rat ECs, produced approximately 10 times as much total somatostatin as did control-transfected ECs (450 +/- 32 vs 49 +/- 10 pmol/L, p < 0.05). The predominant isoform of somatostatin produced was somatostatin-14. Production of somatostatin was stable with passage and did not impair the growth of canine ECs. The failure of rat ECs to produce somatostatin correlated with nonuniform expression of beta-galactosidase, suggesting that promoter silencing was responsible for failure of transgene expression. CONCLUSION:Retroviral gene transfer of somatostatin to canine ECs results in the production of physiologically relevant concentrations of biologically active somatostatin. Significant species differences exist in EC production of somatostatin, with promoter silencing being a potential mechanism of failure of gene expression. Gene therapy strategies using retroviral transfer of somatostatin to ECs may allow somatostatin delivery to focal areas of the vasculature.
journal_name
J Vasc Surgjournal_title
Journal of vascular surgeryauthors
Sarkar R,Finniss S,Dickinson CJ,Stanley JCdoi
10.1016/s0741-5214(98)70278-2subject
Has Abstractpub_date
1998-05-01 00:00:00pages
955-62issue
5eissn
0741-5214issn
1097-6809pii
S0741521498001566journal_volume
27pub_type
杂志文章abstract：:Electronic medical records are gathering significant attention with the recent State and Federal initiatives, Medicare Physician Quality Reporting Initiatives (PQRI), and stimulus funds. The conversion to an electronic environment from the comfortable but inefficient paper record can be confusing, difficult, and costl...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2009.12.052
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVE:The problems with first-generation stent grafts for endovascular aneurysm repair are well known, but their long-term outcome remains to be established. The purpose of the study was to characterize the outcome of patients treated for abdominal aortic aneurysm (AAA) with a first-generation stent graft, Vanguard...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2019.11.027
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to provide, by means of computational fluid dynamics, a comparative analysis after carotid endarterectomy (CEA) between patch graft (PG) and primary closure (PC) techniques performed in real carotid geometries to identify disturbed flow conditions potentially involved in the developme...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2017.08.094
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:Open surgical repair (OSR) for chronic type B aortic dissection (CTBAD) has an associated morbidity and mortality. The role of thoracic endovascular aortic repair (TEVAR) in CTBAD has not been determined. We analyzed our contemporary experience of CTBAD undergoing OSR to identify high-risk patients who may be...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2013.11.002
更新日期：2014-05-01 00:00:00
abstract：BACKGROUND:Endovascular aortic aneurysm repairs (EVARs) with fenestrated (FEVAR) stent grafts are high radiation dose cases, yet no skin injuries were found retrospectively in our 61 cases with a mean peak skin dose (PSD) of 6.8 Gy. We hypothesize that skin injury is under-reported. This study examined deterministic ef...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2014.11.044
更新日期：2015-04-01 00:00:00
abstract：PURPOSE:Although anticoagulation therapy for iliofemoral venous thrombosis prevents pulmonary embolism, it is not designed to avoid the postthrombotic syndrome. Mechanical removal of the thrombus in the form of venous thrombectomy should yield better long-term results. The purpose of our study was to analyze the clinic...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/s0741-5214(97)70249-0
更新日期：1997-03-01 00:00:00
abstract：OBJECTIVE:The Kidney Disease Outcome Quality Initiative and Fistula First Breakthrough Initiative call for the indiscriminate creation of arteriovenous fistulas (AVFs) over arteriovenous grafts (AVGs) without providing patient-specific criteria for vascular access selection. Although the U.S. AVF rate has increased dra...
journal_title：Journal of vascular surgery
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.jvs.2016.10.099
更新日期：2017-04-01 00:00:00
abstract：OBJECTIVE:To achieve an optimal sealing zone during endovascular aneurysm repair, the intended positioning of the proximal end of the endograft fabric should be as close as possible to the most caudal edge of the renal arteries. Some endografts exhibit a small offset between the radiopaque markers and the proximal fabr...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2017.05.092
更新日期：2018-05-01 00:00:00
abstract：:Suture-mediated closure devices are increasingly used for closure of large arteriotomies, such as those required for endovascular aortic repair. One of the commonly encountered situations is persistent arteriotomy oozing after successful closure requiring manual compression. The purpose of this report is to describe a...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2012.09.022
更新日期：2013-02-01 00:00:00
abstract：PURPOSE:A prospective controlled study was undertaken to determine how peripheral vascular disease (PVD) influences flow in the deep veins of the leg. METHODS:Eighty-nine patients with peripheral vascular disease and 35 age-matched control subjects were studied. The popliteal vein diameter and flow velocity were measu...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/s0741-5214(99)70247-8
更新日期：1999-06-01 00:00:00
abstract：:Despite the proven experimental success of endothelial cell seeding of prosthetic vascular grafts, the process has not been widely accepted for clinical use because of its complexity and the need for a relatively large length of autologous vein to provide the requisite number of cells. Using autologous endothelial cel...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1067/mva.1986.avs0030264
更新日期：1986-02-01 00:00:00
abstract：:A 46-year-old woman was admitted with acute abdominal pain radiating to the back. Computed tomography examinations showed a needle-thin perforation of the aorta, opposite the origin of the superior mesenteric artery, with a pseudoaneurysm. A needle-sharp spinal osteophyte was located exactly opposite the perforation. ...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2006.12.070
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:Thrombolysis is a common method in the treatment of lower extremity bypass graft occlusion. The purpose of this study was to investigate the results of thrombolytic therapy in the management of acute bypass graft occlusion and to identify risk factors for technical failure and amputation. METHODS:All patien...
journal_title：Journal of vascular surgery
pub_type： 杂志文章,多中心研究
doi：10.1016/j.jvs.2011.05.008
更新日期：2011-11-01 00:00:00
abstract：PURPOSE:To determine which patients require subfascial endoscopic perforator surgery (SEPS) in addition to saphenous surgery to correct pathologic outward flow within incompetent medial calf perforating veins (IPVs). METHODS:Sixty-two limbs of 47 patients undergoing sapheno-femoral ligation, stripping of the long saph...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/s0741-5214(98)70058-8
更新日期：1998-11-01 00:00:00
abstract：:A retrospective review was undertaken of a random sample (N = 73) comprising 50% of carotid endarterectomies performed during 1986 to evaluate the necessity of routine postoperative intensive care unit (ICU) admission after carotid endarterectomy. Severity of illness was determined with use of the Acute Physiology Sco...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1067/mva.1991.33418
更新日期：1991-12-01 00:00:00
abstract：PURPOSE:Percutaneous angioplasty and stenting (PTAS) is emerging as a therapeutic option for patients with chronic mesenteric ischemia. This study evaluated patency and mortality, and their relationship between degree of vessel occlusion (stenotic or totally occluded), stent characteristics, and comorbidities in patien...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2007.11.046
更新日期：2008-03-01 00:00:00
abstract：:Connective tissue disorders (CTDs) predispose patients to dilation of the entire aorta, resulting in the development of extensive aneurysms. Aortic reconstruction in CTD patients can be challenging and demands specific approaches to ensure initial success and lasting stability of aortic repair. Herein, we describe tec...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2016.04.044
更新日期：2016-08-01 00:00:00
abstract：PURPOSE:The activation of cyclic nucleotide-dependent signaling pathways in vascular smooth muscle is important for the prevention of vein graft spasm and neointimal hyperplasia. Cyclic nucleotide-dependent relaxation is associated with an increase in the phosphorylation of a small heat shock-related protein (HSP20). I...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/s0741-5214(99)70385-x
更新日期：1999-02-01 00:00:00
abstract：:The successful treatment of a brachial artery occlusion caused by a chronic embolizing aneurysm of the anterior humeral circumflex artery is described. In the case of embolizing axillary artery aneurysms, the best therapy is resection of the aneurysm followed by distal thromboembolectomy and vascular reconstruction by...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1067/mva.1991.25235
更新日期：1991-03-01 00:00:00
abstract：PURPOSE:This study was designed to determine whether magnetic resonance angiography (MRA) will allow preoperative management decisions without the need for contrast arteriography in patients with lower extremity ischemia caused by infrainguinal arterial occlusive disease. METHODS:Forty-five patients with lower extremi...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/s0741-5214(96)70241-0
更新日期：1996-05-01 00:00:00
abstract：OBJECTIVE:Hybrid procedures have gained momentum as less invasive operations that can potentially improve outcomes for patients. However, there is a paucity of data comparing hybrid procedures with traditional bypass. This study compares the perioperative outcomes of hybrid and bypass surgery for femoropopliteal (FP) r...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2018.01.058
更新日期：2018-11-01 00:00:00
abstract：:During your preoperative visit, a very wealthy patient, Gill Bates, who donated a wing to the large teaching hospital in which you practice, has an unusual request: He does not want any residents involved with his care. He is scheduled for an open infrarenal aneurysmectomy. He explains that his decision is based on th...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2013.07.099
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVE:Current long-term surveillance after endovascular abdominal aortic aneurysm repair (EVAR) is based on high-resolution contrast-enhanced computed tomography (CT) scans at scheduled, lifelong intervals. The cancer and nephrotoxicity risks of interval CT scanning and prolonged radiation exposure are concerning. ...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2010.06.019
更新日期：2010-11-01 00:00:00
abstract：PURPOSE:Two of three patients with vertebrobasilar stroke harbor a stenosis of the vertebral or basilar arteries. The best treatment for secondary prophylaxis in vertebrobasilar occlusive disease has not been defined. In patients with high-grade stenoses, and especially those refractory to medication, stenting offers t...
journal_title：Journal of vascular surgery
pub_type： 杂志文章,评审
doi：10.1016/j.jvs.2006.02.027
更新日期：2006-06-01 00:00:00
abstract：OBJECTIVE:This study was conducted to determine the outcomes and predictive factors for success during percutaneous endovascular aneurysm repair (PEVAR) using vascular closure devices (VCDs). METHODS:The clinical data of patients who underwent PEVAR between 2005 and 2012 were retrospectively reviewed. Patient demograp...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2013.05.028
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE:We report 5 patients in whom a symptomatic perigraft seroma developed within the aortic sac, without vascular endoleak, after open repair of an abdominal aortic aneurysm (AAA) with a polytetrafluoroethylene (PTFE) graft. We also discuss possible relationships of this phenomenon to endovascular repair of AAAs....
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2004.08.057
更新日期：2004-12-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to examine the anatomic distribution and associated mortality of combat-related vascular injuries comparing them to a contemporary civilian standard. DESIGN:The Joint Trauma Theater Registry (JTTR) was queried to identify patients with major compressible arterial injury (CAI) an...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2012.02.048
更新日期：2012-09-01 00:00:00
abstract：:A 15-year-old male complained of easy fatigability, leg weakness, and pain on exertion with episodes of syncope while playing baseball. His past medical history was significant for aortic coarctation associated with a congenital bovine hypoplastic aortic arch. A recent arteriogram revealed innominate and left common c...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2004.09.035
更新日期：2005-03-01 00:00:00
abstract：:Endovascular treatment of aneurysmal disease has become the predominant form of repair for all aneurysms. Some areas continue to pose specific challenges to stay within the general tenets of successful repair, mainly achieving adequate seal without sacrificing other arterial pathways. Following aortic aneurysm repair,...
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/j.jvs.2013.03.005
更新日期：2014-02-01 00:00:00
abstract：OBJECTIVE:This study was undertaken to describe the technique of transfemoral superselective coil embolization of type II endoleak and its influence on abdominal aortic aneurysm diameter. METHODS:Over 23 months, 104 aortic stent grafts were deployed to exclude abdominal aortic aneurysms, at an academic medical center....
journal_title：Journal of vascular surgery
pub_type： 杂志文章
doi：10.1016/s0741-5214(02)75467-0
更新日期：2003-07-01 00:00:00
