Abstract:
:We explored whether barasertib (AZD1152), a selective Aurora B kinase inhibitor, is a substrate for P-glycoprotein (Pgp, MDR1), breast cancer resistance protein (BCRP), and multidrug resistance protein 2 (MRP2) in vitro. Cell survival, drug transport, and competition experiments with barasertib pro-drug and the more active form of the drug (barasertib-hQPA) were performed using MDCKII (wild type, MDR1, BCRP, and MRP2) and LLCPK (wild type and MDR1) cells and monolayers, and Sf9-BCRP membrane vesicles. Moreover we tested whether P-gp and BCRP affect the oral pharmacokinetics, tissue distribution, and myelotoxicity of barasertib in vivo using Bcrp1(-/-)/Mdr1a/1b (-/-) (triple knockout) and wild type mice. In cell survival experiments expression of BCRP and MDR1 resulted in significant resistance to barasertib. In transwell experiments, barasertib-hQPA was transported by BCRP and MDR1 efficiently. In Sf9-BCRP membrane vesicles, both barasertib and barasertib-hQPA significantly inhibited the BCRP-mediated transport of methotrexate. In contrast, no active transport of barasertib by MRP2 was observed, and overexpression of MRP2 did not affect cytotoxicity of barasertib. In vivo, systemic exposure as well as bioavailability, brain penetration, kidney and liver distribution and myelotoxicity of barasertib-hQPA were statistically significantly increased in Bcrp1(-/-)/Mdr1a/1b(-/-) compared with wild type mice (p<0.001). Barasertib is transported efficiently by P-gp and BCRP/Bcrp1 in vitro. In vivo, genetic deletion of P-gp and BCRP in mice significantly affected pharmacokinetics, tissue distribution and myelotoxicity of barasertib-hQPA. Possible clinical consequences for the observed affinity of barasertib for P-gp and BCRP need to be explored.
journal_name
Invest New Drugsjournal_title
Investigational new drugsauthors
Marchetti S,Pluim D,van Eijndhoven M,van Tellingen O,Mazzanti R,Beijnen JH,Schellens JHdoi
10.1007/s10637-013-9923-1subject
Has Abstractpub_date
2013-10-01 00:00:00pages
1125-35issue
5eissn
0167-6997issn
1573-0646journal_volume
31pub_type
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journal_title:Investigational new drugs
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pub_type: 杂志文章,多中心研究,随机对照试验
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pub_type: 临床试验,杂志文章
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journal_title:Investigational new drugs
pub_type: 杂志文章,多中心研究,随机对照试验
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journal_title:Investigational new drugs
pub_type: 临床试验,杂志文章,多中心研究
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,多中心研究
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journal_title:Investigational new drugs
pub_type: 杂志文章
doi:10.1007/s10637-014-0137-y
更新日期:2014-12-01 00:00:00
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journal_title:Investigational new drugs
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更新日期:2010-12-01 00:00:00
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journal_title:Investigational new drugs
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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journal_title:Investigational new drugs
pub_type: 杂志文章
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journal_title:Investigational new drugs
pub_type: 临床试验,杂志文章
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journal_title:Investigational new drugs
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2011-06-01 00:00:00