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Tools and methodologies capable of isolating and identifying a target molecule for a bioactive compound.

Abstract：

:Elucidating the mechanism of action of bioactive compounds, such as commonly used pharmaceutical drugs and biologically active natural products, in the cells and the living body is important in drug discovery research. To this end, isolation and identification of target protein(s) for the bioactive compound are essential in understanding its function fully. And, development of reliable tools and methodologies capable of addressing efficiently identification and characterization of the target proteins based on the bioactive compounds accelerates drug discovery research. Affinity-based isolation and identification of target molecules for the bioactive compounds is a classic, but still powerful approach. This paper introduces recent progress on affinity chromatography system, focusing on development of practical affinity matrices and useful affinity-based methodologies on target identification. Beneficial affinity chromatography systems with using practical tools and useful methodologies facilitate chemical biology and drug discovery research.

journal_name

Bioorg Med Chem

journal_title

Bioorganic & medicinal chemistry

authors

Sakamoto S,Hatakeyama M,Ito T,Handa H

doi

10.1016/j.bmc.2011.12.022

subject

Has Abstract

pub_date

2012-03-15 00:00:00

pages

1990-2001

issue

6

eissn

0968-0896

issn

1464-3391

pii

S0968-0896(11)01028-5

journal_volume

20

pub_type

杂志文章

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