Microsatellite markers derived from Calophyllum inophyllum (Clusiaceae) expressed sequence tags.

Abstract:

PREMISE OF THE STUDY:Robust markers are required (inter alia) for assessing origins of Calophyllum inophyllum populations on the Bonin Islands, Japan. Therefore, informative expressed sequence tag (EST)-based microsatellite or simple sequence repeat (SSRs) markers in the species were sought. METHODS AND RESULTS:Using 135378 ESTs derived from de novo pyrosequencing, primers for 475 EST-SSRs were developed, 48 of which were tested for PCR amplification. Thirty-six of the 48 primers showed clear amplification, with 23 displaying polymorphism in sampled populations. Expected heterozygosity in the samples from the Bonin Islands and Ryukyu Islands populations ranged from 0.041 to 0.697 and from 0.041 to 0.773, respectively. CONCLUSIONS:As EST-SSRs are potentially tightly linked with functional genes, and reportedly more transferable to related species than anonymous genomic SSRs, the developed primers have utility for future studies of the origins, genetic structure, and conservation of C. inophyllum and related species.

journal_name

Am J Bot

authors

Setsuko S,Uchiyama K,Sugai K,Hanaoka S,Yoshimaru H

doi

10.3732/ajb.1100299

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

e28-32

issue

1

eissn

0002-9122

issn

1537-2197

pii

ajb.1100299

journal_volume

99

pub_type

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