Abstract:
:The structural conversion of the prion protein (PrP) from the normal cellular isoform (PrP(C)) to the posttranslationally modified form (PrP(Sc)) is thought to relate to Cu²⁺ binding to histidine (H) residues. Traditionally, the binding of metals to PrP has been investigated by monitoring the conformational conversion using circular dichroism (CD). In this study, the metal-binding ability of 21 synthetic peptides representing regions of human PrP(C) was investigated by column switch high-performance liquid chromatography (CS-HPLC). The CS-HPLC system is composed of a metal chelate affinity column and an octadecylsilica (ODS) reversed-phase column that together enable the identification of metal-binding regardless of conformational conversion. Synthetic peptides were designed with respect to the position of H residues as well as the secondary structure of human PrP (hPrP). The ability of the octapeptide (PHGGGWGQ)-repeating region (OP-repeat) to bind metals was analyzed by CS-HPLC and supported by CD analysis, and indicated that CS-HPLC is a reliable and useful method for measuring peptide metal-binding. Peptides from the middle region of hPrP showed a high affinity for Cu²⁺, but binding to Zn²⁺, Ni²⁺, and Co²⁺ was dependent on peptide length. C-Terminal peptides had a lower affinity for Cu²⁺, Zn²⁺, Ni²⁺, and Co²⁺ than OP-repeat region peptides. Interestingly, hPrP193-230, which contained no H residues, also bound to Cu²⁺, Zn²⁺, Ni²⁺, and Co²⁺, indicating that this region is a novel metal-binding site in the C-terminal region of PrP(C). The CS-HPLC method described in this study is useful and convenient for assessing metal-binding affinity and characterizing metal-binding peptides or proteins.
journal_name
Chem Pharm Bull (Tokyo)journal_title
Chemical & pharmaceutical bulletinauthors
Kojima A,Mabuchi Y,Konishi M,Okihara R,Nagano M,Akizawa Tdoi
10.1248/cpb.59.965subject
Has Abstractpub_date
2011-01-01 00:00:00pages
965-71issue
8eissn
0009-2363issn
1347-5223pii
JST.JSTAGE/cpb/59.965journal_volume
59pub_type
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