Engineered cartilage maturation regulates cytokine production and interleukin-1β response.

Abstract:

BACKGROUND:Because the injured joint has an actively inflammatory environment, the survival and repair potential of cartilage grafts may be influenced by inflammatory processes. Understanding the interactions of those processes with the graft may lead to concepts for pharmacologic or surgical solutions allowing improved cartilage repair. QUESTIONS/PURPOSES:We asked whether the maturation level of cartilaginous tissues generated in vitro by expanded human articular chondrocytes (HACs) modulate (1) the spontaneous production of cytokines and (2) the response to interleukin (IL)-1β. METHODS:Twelve pellets/donor prepared with monolayer-expanded HACs (n = 6 donors) were evaluated at six different culture times for mRNA expression (n = 72) and spontaneous baseline release of monocyte chemoattractant protein (MCP)-1, IL-8, and transforming growth factor (TGF)-β1 (n = 72). We cultured 24 pellets/donor from each of four donors for 1 or 14 days (defined as immature and mature, respectively) and exposed the pellets to IL-1β for 3 days. MCP-1, IL-8, TGF-β1, and metalloprotease (MMP)-1 and MMP-13 were quantified in pellets and culture supernatants. RESULTS:By increasing culture time, the spontaneous release of IL-8 and MCP-1 decreased (12.0- and 5.5-fold, respectively), whereas that of TGF-β1 increased (5.4-fold). As compared with immature pellets, mature pellets responded to IL-1β by releasing lower amounts of MMP-1 (2.9-fold) and MMP-13 (1.7-fold) and increased levels of IL-8, MCP-1, and TGF-β1 (1.5-, 5.0-, and 7.5-fold, respectively). IL-8 and MCP-1 promptly returned to baseline on withdrawal of IL-1β. CONCLUSIONS:Our observations suggest more mature cartilaginous tissues are more resistant to IL-1β exposure and can activate chemokines required to initiate tissue repair processes. CLINICAL RELEVANCE:The implantation of more mature cartilaginous tissues might provide superior graft survival and improve/accelerate cartilage repair.

journal_name

Clin Orthop Relat Res

authors

Francioli S,Cavallo C,Grigolo B,Martin I,Barbero A

doi

10.1007/s11999-011-1826-x

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

2773-84

issue

10

eissn

0009-921X

issn

1528-1132

journal_volume

469

pub_type

杂志文章
  • The treatment of fractures of the hip by Roger Anderson well-leg traction.

    abstract::In recent years the treatment of fractures of the hip in elderly patients has been almost exclusive surgical. However, there are occasions when either because of medical conditions or other surgical problems it is not possible to operate on these patients. An alternative method of handling such patients is the use of ...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Pierce RO Jr,Powell SG

    更新日期:1980-09-01 00:00:00

  • Acetabular reconstruction with bone impaction grafting and a cemented cup: 20 years' experience.

    abstract::Acetabular bone stock loss compromises the outcome in primary and revision total hip arthroplasty. In 1979, a biologic method was introduced with tightly impacted cancellous allografts in combination with a cemented polyethylene cup for acetabular reconstruction. With this technique, it is possible to replace the loss...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1097/00003086-200112000-00023

    authors: Schreurs BW,Slooff TJ,Gardeniers JW,Buma P

    更新日期:2001-12-01 00:00:00

  • Age-associated changes in cartilage matrix: implications for tissue repair.

    abstract::The structure of the extracellular matrix of articular cartilage varies considerably with age. These changes are attributable to variations in molecular abundance and structure, and they can affect all the matrix components, but none more so than the proteoglycans. Some of these changes are attributable to variations ...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章,评审

    doi:10.1097/00003086-200110001-00015

    authors: Roughley PJ

    更新日期:2001-10-01 00:00:00

  • High methodologic quality but poor applicability: assessment of the AAOS guidelines using the AGREE II instrument.

    abstract:BACKGROUND:The American Academy of Orthopaedic Surgeons (AAOS) is a globally recognized leader in musculoskeletal and orthopaedic education. Clinical guidelines are one important focus of the AAOS' educational efforts. Although their recommendations sometimes generate controversy, a critical appraisal of the overall qu...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1007/s11999-014-3530-0

    authors: Sabharwal S,Patel NK,Gauher S,Holloway I,Athanasiou T

    更新日期:2014-06-01 00:00:00

  • Pelvic lysis and polyethylene wear at 5-8 years in an uncemented total hip.

    abstract::The clinical and radiographic results of 160 primary, uncemented porous coated total hip replacements performed at 3 teaching hospitals were reviewed. Followup was obtained in 132 of 148 (89%) nondeceased patients. The acetabular component is a full hemisphere, fabricated of cobalt chrome with a sintered bead coating ...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Barrack RL,Folgueras A,Munn B,Tvetden D,Sharkey P

    更新日期:1997-02-01 00:00:00

  • Spontaneous fractures in the renal graft recipient: a discriminant analysis of 144 cases.

    abstract::In a search for factors predisposing to the occurrence of spontaneous fractures in the renal organ transplant patient, a comparison has been made of 38 recipients sustaining spontaneous fractures and 106 without this complication. The comparison was made with regard to the clinical findings, the immunosuppressive ther...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Elmstedt E

    更新日期:1982-01-01 00:00:00

  • Subtotal arthrodeses of the wrist joint.

    abstract::The goal of subtotal arthrodesis of the wrist is to provide a stable and pain-free joint, with preservation of a limited but useful range of motion. Experience indicates that the available mobility of the joints that are left unfused tends to increase with time and use of the extremity, and that accelerated wear of th...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Taleisnik J

    更新日期:1984-07-01 00:00:00

  • Recurrent hemarthrosis associated with gout.

    abstract::Gout commonly occurs as an acute arthritis. In a 64-year-old man, gout was associated with a proliferative hemosiderin-laden synovitis. The association of recurrent atraumatic hemarthrosis seems not to have been reported in the literature on gout. ...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Martin DJ,Merenda G,McDonald DJ,Sundaram M

    更新日期:1992-04-01 00:00:00

  • Accuracy of correction of complex lower-extremity deformities by the Ilizarov method.

    abstract::Gradual mechanical distraction with the Ilizarov external fixator was used on 28 limbs in 23 patients to correct complex lower-extremity deformities of diverse causes. To determine the accuracy of realignment and deformity correction, the charts and radiographs were reviewed retrospectively. Preoperative long-standing...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Tetsworth KD,Paley D

    更新日期:1994-04-01 00:00:00

  • Aneurysmal bone cyst. A population based epidemiologic study and literature review.

    abstract::Aneurysmal bone cyst is a rare nonneoplastic expansile osteolytic bone lesion of unknown etiology. To the best of the authors' knowledge, no epidemiologic study concerning its incidence has been reported. The authors performed a retrospective, population based analysis of 94 patients with primary aneurysmal bone cyst ...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章,评审

    doi:

    authors: Leithner A,Windhager R,Lang S,Haas OA,Kainberger F,Kotz R

    更新日期:1999-06-01 00:00:00

  • Does an Algorithmic Approach to Using Brachytherapy and External Beam Radiation Result in Good Function, Local Control Rates, and Low Morbidity in Patients With Extremity Soft Tissue Sarcoma?

    abstract:BACKGROUND:High-dose-rate brachytherapy (HDR-BT) and external-beam radiation therapy (EBRT) are two modalities used in the treatment of soft tissue sarcoma. Previous work at our institution showed early complications and outcomes for patients treated with HDR-BT, EBRT, or a combination of both radiation therapy modalit...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1007/s11999.0000000000000079

    authors: Klein J,Ghasem A,Huntley S,Donaldson N,Keisch M,Conway S

    更新日期:2018-03-01 00:00:00

  • Cementless acetabular replacement in the young. A 5- to 10-year prospective study.

    abstract::This article presents a prospective clinical and radiologic review of 55 total hip replacements in 50 patients in which the cementless Harris-Galante porous-coated acetabular component was used. All patients were 50 years of age or younger. The cups were inserted by 15 different surgeons. The minimum followup was 5 ye...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1097/00003086-200007000-00021

    authors: Dunkley AB,Eldridge JD,Lee MB,Smith EJ,Learmonth ID

    更新日期:2000-07-01 00:00:00

  • Retained bullet removal in civilian pelvis and extremity gunshot injuries: a systematic review.

    abstract:BACKGROUND:Although gunshot injuries are relatively common, there is little consensus about whether retained bullets or bullet fragments should be removed routinely or only in selected circumstances. QUESTIONS/PURPOSES:We performed a systematic review of the literature to answer the following questions: (1) Is bullet ...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章,评审

    doi:10.1007/s11999-013-3260-8

    authors: Riehl JT,Sassoon A,Connolly K,Haidukewych GJ,Koval KJ

    更新日期:2013-12-01 00:00:00

  • Providing outreach continuing education in countries with limited resources.

    abstract::Obtaining continuing education can be difficult in countries with limited resources. Members of the Pediatric Orthopaedic Society of North America (POSNA) have developed a program for providing outreach continuing education courses in pediatric orthopaedics. POSNA members provide their own transportation to the host c...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1007/s11999-008-0381-6

    authors: Wilkins KE

    更新日期:2008-10-01 00:00:00

  • Improving web site performance using commercially available analytical tools.

    abstract:BACKGROUND:It is easy to accurately measure web site usage and to quantify key parameters such as page views, site visits, and more complex variables using commercially available tools that analyze web site log files and search engine use. This information can be used strategically to guide the design or redesign of a ...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1007/s11999-010-1333-5

    authors: Ogle JA

    更新日期:2010-10-01 00:00:00

  • Extraosseous blood supply to the neonatal femoral head.

    abstract::The extraosseous blood supply to the femoral head was studied by microdissection of latex-injected vessels in 19 neonatal hips. The fetuses were of black African descent. Although the general pattern was similar to that described by previous investigators, in seven of the specimens the largest contribution to the bloo...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: O'Hara JP 3rd,Dommisse GF

    更新日期:1983-04-01 00:00:00

  • Anatomic considerations for safe placement of calcaneal pins.

    abstract::Placement of a Steinmann pin in the calcaneus is indicated in various orthopaedic conditions. Planning the point of entry and the direction of transcalcaneal pin insertion is crucial for avoidance of neurovascular injury, tendon injury, and subtalar joint violation. Fifteen cadaveric feet were studied in which transfi...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1097/00003086-199611000-00033

    authors: Mekhail AO,Ebraheim NA,Heck BE,Yeasting RA

    更新日期:1996-11-01 00:00:00

  • The alpha-defensin test for periprosthetic joint infection outperforms the leukocyte esterase test strip.

    abstract:BACKGROUND:Synovial fluid biomarkers have demonstrated diagnostic accuracy surpassing the currently used diagnostic tests for periprosthetic joint infection (PJI). QUESTIONS/PURPOSES:The purpose of this study is to directly compare the sensitivity and specificity of the synovial fluid α-defensin immunoassay to the leu...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1007/s11999-014-3722-7

    authors: Deirmengian C,Kardos K,Kilmartin P,Cameron A,Schiller K,Booth RE Jr,Parvizi J

    更新日期:2015-01-01 00:00:00

  • Conversion of Hemiarthroplasty to THA Carries an Increased Risk of Reoperation Compared With Primary and Revision THA.

    abstract:BACKGROUND:There is limited information on the complications and costs of conversion THA after hemiarthroplasty for femoral neck fractures. Previous studies have found that patients undergoing conversion THA experience higher risk complications, but it has been difficult to quantify the risk because of small sample siz...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1097/CORR.0000000000000702

    authors: Hernandez NM,Fruth KM,Larson DR,Kremers HM,Sierra RJ

    更新日期:2019-06-01 00:00:00

  • Proximal humerus fracture sequelae: impact of a new radiographic classification on arthroplasty.

    abstract:UNLABELLED:Our goal was to analyze the results of unconstrained shoulder replacement in a large series of sequelae of proximal humeral fractures in order to validate a previously described surgical classification. In a multicenter study, we retrospectively evaluated 203 patients with sequelae of proximal humeral fractu...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章,多中心研究

    doi:10.1097/01.blo.0000195679.87258.6e

    authors: Boileau P,Chuinard C,Le Huec JC,Walch G,Trojani C

    更新日期:2006-01-01 00:00:00

  • Gentamicin-PMMA beads. Pharmacokinetic and nephrotoxicological study.

    abstract::Five patients treated with gentamicin-polymethylmethacrylate (PMMA) beads were monitored during the treatment of nine to 14 days. Kinetic data of the gentamicin delivery were gathered constantly by multiple samples of blood and urine. The treatment with 48-360 beads caused a gentamicin serum concentration of 0.03-0.4 ...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Walenkamp GH,Vree TB,van Rens TJ

    更新日期:1986-04-01 00:00:00

  • Staples equal sutures for skin closure after soft tissue tumor resection.

    abstract:BACKGROUND:Wound closure accounts for a relatively constant portion of the time required to complete a surgical case. Both longer closure times and wound infections contribute to higher medical costs and patient morbidity. QUESTIONS/PURPOSES:We therefore determined whether (1) biologic and treatment factors greater in...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1007/s11999-012-2524-z

    authors: Moore DC,Sellers MH,Archer KR,Schwartz HS,Holt GE

    更新日期:2013-03-01 00:00:00

  • Revision of broken knee megaprostheses: new solution to old problems.

    abstract:BACKGROUND:Low-cost indigenous megaprostheses used in the developing world are prone to mechanical failure but the frequency and causes are not well established. QUESTIONS/PURPOSES:We retrospectively analyzed the causes of failure, particularly design, and suggest changes to reduce the breakage. We also report our exp...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1007/s11999-010-1409-2

    authors: Agarwal M,Gulia A,Ravi B,Ghyar R,Puri A

    更新日期:2010-11-01 00:00:00

  • Avulsion fractures of the posterior cruciate ligament of the knee. An experimental percutaneous rigid fixation technique under arthroscopic control.

    abstract::A posterior cruciate ligament avulsion fracture (PCLAF) of the knee can be repaired through a large approach. A new technique based on internal fixation under arthroscopic control employed a posterolateral puncture. The fracture was reduced and the osseous fragment transfixed using a new forceps guide. The osseous fra...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Martinez-Moreno JL,Blanco-Blanco E

    更新日期:1988-12-01 00:00:00

  • Temporary antibiotic cement-covered gamma nail spacer for an infected nonunion of the proximal femur.

    abstract::We report the case of an infected nonunion of the proximal femoral in an elderly patient. There was extensive involvement of the entire proximal femur precluding salvage. An impromptu use of a cephalomedullary nail coated with antibiotic-laden bone cement is described, followed by reimplantation with a revision-type p...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1097/01.blo.0000238779.12069.e1

    authors: Rodriguez H,Ziran BH

    更新日期:2007-01-01 00:00:00

  • Local control and survival from the Cooperative Osteosarcoma Study Group studies of the German Society of Pediatric Oncology and the Vienna Bone Tumor Registry.

    abstract::The use of aggressive chemotherapy undoubtedly has brought about a dramatic increase in the cure rate of osteosarcoma. The authors' investigations have increased the authors' knowledge of chemotherapy for osteosarcoma, the differential efficacy of currently used agents, and the pronounced schedule dependency and relat...

    journal_title:Clinical orthopaedics and related research

    pub_type: 临床试验,杂志文章,多中心研究,评审

    doi:

    authors: Winkler K,Bieling P,Bielack S,Delling G,Dose C,Jürgens H,Kotz R,Ritter J,Salzer-Kuntschik M

    更新日期:1991-09-01 00:00:00

  • Osteoid osteoma under the age of five years. The difficulty of diagnosis.

    abstract::Ninety-one cases of histologically confirmed osteoid osteoma were collected during an 11-year period; of them, seven (7.6%) had onset of symptoms while younger than five years of age. Special diagnostic difficulties were found in this specific age group: most cases were misdiagnosed or diagnosed incorrectly. The time ...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Kaweblum M,Lehman WB,Bash J,Strongwater A,Grant AD

    更新日期:1993-11-01 00:00:00

  • Clinical and Molecular Analysis of Pathologic Fracture-associated Osteosarcoma: MicroRNA profile Is Different and Correlates with Prognosis.

    abstract:BACKGROUND:MicroRNAs are small, noncoding RNAs that regulate the expression of posttranslational genes. The presence of some specific microRNAs has been associated with increased risk of both local recurrence and metastasis and worse survival in patients with osteosarcoma. Pathologic fractures in osteosarcoma are consi...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1097/CORR.0000000000000867

    authors: Lozano Calderón SA,Garbutt C,Kim J,Lietz CE,Chen YL,Bernstein K,Chebib I,Nielsen GP,Deshpande V,Rubio R,Wang YE,Quackenbush J,Delaney T,Raskin K,Schwab J,Cote G,Spentzos D

    更新日期:2019-09-01 00:00:00

  • Interlocking intramedullary nails. An improved method of screw placement combining image intensification and laser light.

    abstract::Insertion of distal interlocking screws in femoral nails can be technically demanding and may entail substantial exposure. A method of insertion of femoral interlocking screws that uses a laser guiding system in addition to the standard image intensifier was used in an attempt to improve the accuracy of distal screw p...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:

    authors: Goulet JA,Londy F,Saltzman CL,Matthews LS

    更新日期:1992-08-01 00:00:00

  • Plastic femoral deformity in a 9-year-old Boy.

    abstract::We report the case of a 9-year-old boy who sustained a closed middiaphyseal plastic femoral deformation after a motor vehicle accident. The patient was treated nonoperatively with protected weightbearing to the involved extremity. Although he showed symptomatic improvement at early and intermediate followups, the pati...

    journal_title:Clinical orthopaedics and related research

    pub_type: 杂志文章

    doi:10.1097/BLO.0b013e3181342851

    authors: Lavery MR,Huang M,Amendola A

    更新日期:2007-09-01 00:00:00