Inactivation of Staphylococcus aureus and Escherichia coli by the synergistic action of high hydrostatic pressure and dissolved CO₂.

Abstract:

:This study focused on the synergistic inactivation effects of combined treatment of HHP and dissolved CO₂ on microorganisms. The aim was to reduce the treatment pressure of the traditional HHP technology and make it more economically feasible. The combined treatment showed a strong bactericidal effect on Staphylococcus aureus and Escherichia coli in liquid culture, which usually have high levels of barotolerance under pressure alone. To identify the influence of CO₂, a new setup to dissolve, retain and measure the concentration of CO₂ was constructed. The results demonstrated that an inactivation rate of more than 8 log units was obtained for E. coli both at 300 MPa with 1.2 NL/L CO₂ and at 250 MPa with 3.2 NL/L CO₂, while only 2.2 and 1.8 log reductions were observed at 300 MPa and 250 MPa, respectively, for the HHP treatments alone. For S. aureus, the inactivation rate of more than 7 log units was found at 350 MPa with 3.8 NL/L CO₂, while only a 0.9 log reduction was achieved at this pressure in the absence of CO₂. The SEM photographs showed seriously deformed cells after the synergistic treatments. In contrast, the cells treated with individual HHP maintained a relatively smooth surface with invaginations. Propidium iodide staining and fluorescence observation was performed after pressure treatments. The results demonstrated that the combination of CO₂ with HHP also promoted pressure induced cell membrane permeabilization greatly. It was deduced that the enrichment of CO₂ on the cell surface and its penetration into the cells at high pressure accounted for the membrane damage and cell death.

journal_name

Int J Food Microbiol

authors

Wang L,Pan J,Xie H,Yang Y,Lin C

doi

10.1016/j.ijfoodmicro.2010.09.006

subject

Has Abstract

pub_date

2010-11-15 00:00:00

pages

118-25

issue

1

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(10)00510-6

journal_volume

144

pub_type

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