Quantitative image analysis to characterize the dynamics of Listeria monocytogenes biofilms.

Abstract:

:This work shows that the combination of two-dimensional (2D) and three-dimensional (3D) analyses of images acquired by confocal laser scanning microscopy facilitates the quantitative spatiotemporal characterization of architectures formed by Listeria monocytogenes biofilms. In particular, the analysis of structural features such as maximum thickness, biovolume, areal porosity and maximum diffusion distance allowed elucidating differences in biofilm formation of three L. monocytogenes strains (L1A1, CECT5873 and CECT4032). The analysis showed a common sequence for all strains. In the first phase, independent clusters evolve to interconnected clusters and honeycomb-like structures. Flat biofilms characterized the second phase. The structures disappear in the third phase. Nevertheless, the duration of the phases differed from strain to strain. L1A1 strain exhibited the slowest dynamics and the thickest biofilms while the strain CECT4032 presented the faster dynamics and the thinnest biofilms. Also, the number of dead cells varies significantly from strain to strain. From the results of the analysis, it can be concluded that 2D parameters are critical to differentiating morphological features while 3D parameters ease the interpretation and comparative study of the different phases during the life cycle of biofilms.

journal_name

Int J Food Microbiol

authors

Mosquera-Fernández M,Sanchez-Vizuete P,Briandet R,Cabo ML,Balsa-Canto E

doi

10.1016/j.ijfoodmicro.2016.07.015

subject

Has Abstract

pub_date

2016-11-07 00:00:00

pages

130-7

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(16)30354-3

journal_volume

236

pub_type

杂志文章
  • The fate of Escherichia coli O157 in soil and its potential to contaminate drinking water.

    abstract::The survival and transport of Escherichia coli and E. coli O157 after cattle slurry application were studied on drained plots in both grassland and arable stubble at three sites in Scotland. Leaching losses were between 0.2% and 10% of total E. coli and were dependent on rainfall. Recovery of E. coli in grass and soil...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(00)00508-0

    authors: Ogden LD,Fenlon DR,Vinten AJ,Lewis D

    更新日期:2001-05-21 00:00:00

  • Depuration dynamics of viruses in shellfish.

    abstract::The consumption of shellfish has been associated with viral infections even in cases where shellfish complied with the current regulation, which is based on bacterial analysis. In this study, depuration rates of potential indicators and human viruses have been analysed in order to study the use of complementary parame...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(02)00052-1

    authors: Muniain-Mujika I,Girones R,Tofiño-Quesada G,Calvo M,Lucena F

    更新日期:2002-07-25 00:00:00

  • Antibacterial activity of selected fatty acids and essential oils against six meat spoilage organisms.

    abstract::The antibacterial activity of selected fatty acids and essential oils was examined against two gram-negative (Pseudomonas fluorescens and Serratia liquefaciens) and four gram-positive (Brochothrix thermosphacta, Carnobacterium piscicola, Lactobacillus curvatus, and Lactobacillus sake) bacteria involved in meat spoilag...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(97)00070-6

    authors: Ouattara B,Simard RE,Holley RA,Piette GJ,Bégin A

    更新日期:1997-07-22 00:00:00

  • Antibiotic resistance among coliform bacteria isolated from carcasses of commercially slaughtered chickens.

    abstract::A total of 322 coliform bacteria Escherichia coli, Enterobacter spp., Citrobacter spp., Klebsiella spp. and Serratia spp., were isolated from 50 carcasses of commercially slaughtered chickens. Their resistance to ampicillin, tetracycline, gentamicin, chloramphenicol, cephalotine, cotrimoxazole, nalidixic acid and nitr...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/0168-1605(90)90029-5

    authors: Turtura GC,Massa S,Ghazvinizadeh H

    更新日期:1990-12-01 00:00:00

  • Effect of Bifidobacterium thermacidophilum probiotic feeding on enterohemorrhagic Escherichia coli O157:H7 infection in BALB/c mice.

    abstract::The effectiveness of Bifidobacterium thermacidophilum RBL 71 as a probiotic against enterohemorrhagic Escherichia coli O157:H7 infection was studied using a murine model. BALB/c mice were fed the probiotic for 7 days before or after single challenge with E. coli O157:H7. Fecal B. thermacidophilum RBL 71 and E. coli O1...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2006.04.041

    authors: Gagnon M,Kheadr EE,Dabour N,Richard D,Fliss I

    更新日期:2006-08-15 00:00:00

  • Application of real-time RT-PCR to study gene expression in active dry yeast (ADY) during the rehydration phase.

    abstract::During the industrial production of active dry yeast (ADY) and its subsequent use in winemaking, the yeast cell is subjected to drastic environmental changes that force it to undergo extensive metabolic modifications and changes in gene expression. In this study, we describe the use of real-time reverse transcription-...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2008.10.027

    authors: Vaudano E,Costantini A,Cersosimo M,Del Prete V,Garcia-Moruno E

    更新日期:2009-01-31 00:00:00

  • Serotype distribution and antibiotic resistance of Salmonella in food-producing animals in Shandong province of China, 2009 and 2012.

    abstract::The aims of this study were to investigate the serotype distribution, genetic relationships and antibiotic resistance of Salmonella from food-producing animals in Shandong province of China in 2009 and 2012. A total of 362 out of 1825 samples from chickens, 53 out of 445 samples from ducks, and 50 out of 692 samples f...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2014.03.030

    authors: Lai J,Wu C,Wu C,Qi J,Wang Y,Wang H,Liu Y,Shen J

    更新日期:2014-06-16 00:00:00

  • High incidence of cold-tolerant Clostridium frigoriphilum and C. algidicarnis in vacuum-packed beef on retail sale in Germany.

    abstract::Sixty vacuum-packed beef samples retailed in Germany were investigated for the occurrence of cold-tolerant Clostridium spp. After a storage period at 4 °C for eight weeks, meat juice from all samples was processed for culturing, DNA extraction and SYBR green qPCR for Clostridium species. After that, a previously devel...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2021.109053

    authors: Mang S,Schwaiger K,Lindner R,Gareis M,Dorn-In S

    更新日期:2021-01-12 00:00:00

  • Detection of Escherichia coli O157 in bovine meat products in northern Italy.

    abstract::Tests for Escherichia coli and E. coli O157 were carried out on meat samples collected from randomly chosen stores throughout the city of Bologna and suburban areas. The samples consisted of 25 g of loose minced beef, sometimes already shaped into meatballs or hamburgers, some of which were mixed with vegetables. The ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(03)00308-8

    authors: Stampi S,Caprioli A,De Luca G,Quaglio P,Sacchetti R,Zanetti F

    更新日期:2004-02-01 00:00:00

  • In vitro fermentation of different fructo-oligosaccharides by Bifidobacterium strains for the selection of synbiotic combinations.

    abstract::The use of selected probiotics, prebiotics and/or synbiotics, constitute an interesting dietary strategy for intestinal microbiota modulation in case of dysbiosis. Species of the genus Bifidobacterium are among the most currently used probiotics for human consumption since they have shown beneficial effects in the pre...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2016.11.011

    authors: Valdés-Varela L,Ruas-Madiedo P,Gueimonde M

    更新日期:2017-02-02 00:00:00

  • Shelf-life of minimally processed lettuce and cabbage treated with gaseous chlorine dioxide and cysteine.

    abstract::Gaseous ClO2 was evaluated for effectiveness in prolonging the shelf-life of minimally processed (MP) lettuce and MP cabbage, previously immersed in a cysteine solution in order to inhibit browning occurring during ClO2 treatment. Each vegetable was shredded, washed, and separated in two portions, one to be treated wi...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2007.11.036

    authors: Gómez-López VM,Ragaert P,Jeyachchandran V,Debevere J,Devlieghere F

    更新日期:2008-01-15 00:00:00

  • Validation of standard method EN ISO 19343 for the detection and quantification of histamine in fish and fishery products using high-performance liquid chromatography.

    abstract::In contaminated fish, bacterial decarboxylases produce histamine from histidine, thereby causing scombroid fish poisoning. European Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs requires using a fully validated, standardized reference HPLC method for detecting and quantifying histamine. After...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2018.07.023

    authors: Duflos G,Inglebert G,Himber C,Degremont S,Lombard B,Brisabois A

    更新日期:2019-01-02 00:00:00

  • Presence and survival of Escherichia coli O157:H7 on lettuce leaves and in soil treated with contaminated compost and irrigation water.

    abstract::Escherichia coli O157:H7 outbreaks associated with produce consumption have brought attention to contaminated compost manure, and polluted irrigation water as potential sources of pathogens for the contamination of these crops. The aim of this study was to determine the potential transfer of E. coli O157:H7 from soil ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2012.03.014

    authors: Oliveira M,Viñas I,Usall J,Anguera M,Abadias M

    更新日期:2012-05-15 00:00:00

  • Microbiology of raw milk in New Zealand.

    abstract::The results of this study demonstrate the occurrence of the non-spore-forming pathogens, Staphylococcus aureus, Escherichia coli (total count and O157:H7), Listeria, Campylobacter and Salmonella, in New Zealand's raw milk supply. Samples of raw milk were collected monthly within five major dairying regions over one ye...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2012.03.031

    authors: Hill B,Smythe B,Lindsay D,Shepherd J

    更新日期:2012-07-02 00:00:00

  • Antifungal activity of food additives in vitro and as ingredients of hydroxypropyl methylcellulose-lipid edible coatings against Botrytis cinerea and Alternaria alternata on cherry tomato fruit.

    abstract::The antifungal activity of food additives or 'generally recognized as safe' (GRAS) compounds was tested in vitro against Botrytis cinerea and Alternaria alternata. Radial mycelial growth of each pathogen was measured in PDA Petri dishes amended with food preservatives at 0.2, 1.0, or 2.0% (v/v) after 3, 5, and 7 days ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2013.08.001

    authors: Fagundes C,Pérez-Gago MB,Monteiro AR,Palou L

    更新日期:2013-09-16 00:00:00

  • Induction of IFN-gamma and IL-1 alpha production in macrophages stimulated with phosphopolysaccharide produced by Lactococcus lactis ssp. cremoris.

    abstract::The induction of interferon (IFN) and interleukin-1 (IL-1) production in murine macrophages by a phosphopolysaccharide, produced by a dairy lactic acid bacteria, Lactococcus lactis ssp. cremoris, was investigated. When the phosphopolysaccharide was added into macrophage cultures at concentrations from 1 to 200 microgr...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/0168-1605(96)00968-3

    authors: Kitazawa H,Itoh T,Tomioka Y,Mizugaki M,Yamaguchi T

    更新日期:1996-08-01 00:00:00

  • Evaluation of pulsed light treatments on inactivation of Salmonella on blueberries and its impact on shelf-life and quality attributes.

    abstract::Blueberry have a short shelf life when fully ripe and susceptible to contamination of various pathogens. Our study investigated the effect of pulsed light (PL) on inactivation of Salmonella on blueberries and its impact on shelf-life, quality attributes and health-benefit compounds of blueberries. Dry PL (6J/cm2) and ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2017.08.012

    authors: Cao X,Huang R,Chen H

    更新日期:2017-11-02 00:00:00

  • Salmonella spp. on pork at cutting plants and at the retail level and the influence of particular risk factors.

    abstract::This article describes the contamination of pork with Salmonella spp. in cutting plants and butchers' shops in The Netherlands and quantifies the influence of several risk factors. When contaminated carcasses are being processed, the main risk factors regarding cross contamination are inapt cleaning and disinfection (...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(98)00122-6

    authors: Berends BR,Van Knapen F,Mossel DA,Burt SA,Snijders JM

    更新日期:1998-11-10 00:00:00

  • Development of blood-yolk-polymyxin B-trimethoprim agar for the enumeration of Bacillus cereus in various foods.

    abstract::Blood-yolk-polymyxin B-trimethoprim agar (BYPTA) was developed by the addition of egg yolk, laked horse blood, sodium pyruvate, polymyxin B, and trimethoprim, and compared with mannitol-yolk-polymyxin B agar (MYPA) for the isolation and enumeration of Bacillus cereus (B. cereus) in pure culture and various food sample...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2013.04.015

    authors: Kim DH,Kim H,Chon JW,Moon JS,Song KY,Seo KH

    更新日期:2013-07-15 00:00:00

  • Antimicrobial resistance observed in Escherichia coli strains isolated from fecal samples of cattle and pigs in Korea during 2003-2004.

    abstract::A total of 744 Escherichia coli strains isolated from 830 fecal samples of healthy cattle and pigs in all provinces of Korea were examined for resistance to 16 antimicrobials. The most frequently observed resistance in cattle isolates was to tetracycline (30.5%), followed by resistance to streptomycin (20.4%), ampicil...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2006.12.014

    authors: Lim SK,Lee HS,Nam HM,Cho YS,Kim JM,Song SW,Park YH,Jung SC

    更新日期:2007-05-10 00:00:00

  • Structural model requirements to describe microbial inactivation during a mild heat treatment.

    abstract::The classical concept of D and z values, established for sterilisation processes, is unable to deal with the typical non-loglinear behaviour of survivor curves occurring during the mild heat treatment of sous vide or cook-chill food products. Structural model requirements are formulated, eliminating immediately some c...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(00)00362-7

    authors: Geeraerd AH,Herremans CH,Van Impe JF

    更新日期:2000-09-10 00:00:00

  • Influence of water activity and temperature on the accumulation of zearalenone in corn.

    abstract::The influence of water activity (aw) and temperature on the zearalenone biosynthesis in corn has been examined. Viable corn kernels were conditioned at different values of water activity (0.90, 0.95 and 0.97), inoculated with Fusarium graminearum and incubated at different temperatures. Zearalenone was determined at s...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/0168-1605(88)90078-5

    authors: Montani ML,Vaamonde G,Resnik SL,Buera P

    更新日期:1988-02-01 00:00:00

  • Impact of cleaning and disinfection on the non-culturable and culturable bacterial loads of food-contact surfaces at a beef processing plant.

    abstract::We assessed the impact of industrial cleaning and disinfection (C&D) on colony-forming units (CFUs), viable (culturable and viable but non-culturable) cells and on total cells (viable and dead cells). Bacterial loads on polyvinyl chloride (PVC) and stainless steel surfaces in a cutting room at a beef processing plant ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2012.07.014

    authors: Khamisse E,Firmesse O,Christieans S,Chassaing D,Carpentier B

    更新日期:2012-08-17 00:00:00

  • Experimental effect of ozone upon the microbial flora of commercially produced dairy fermented products.

    abstract::Ozone was used to control spoilage microorganisms during the manufacturing of dairy products. Ozone stream was applied onto the surface of freshly filled yoghurt cups just before storage for curd development in order to prevent cross contamination from spoilage airborne microorganisms. Accordingly, brine solution was ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2017.01.018

    authors: Alexopoulos A,Plessas S,Kourkoutas Y,Stefanis C,Vavias S,Voidarou C,Mantzourani I,Bezirtzoglou E

    更新日期:2017-04-04 00:00:00

  • Seasonal and tissue distribution of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, in retail freshwater fish in Hong Kong.

    abstract::Laribacter hongkongensis, a recently discovered bacterium associated with community-acquired gastroenteritis, has been found in the intestines of freshwater fish. To better understand the epidemiology and ecology of the bacterium, we carried out a surveillance study to investigate possible seasonal variation in the re...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2006.07.017

    authors: Lau SK,Woo PC,Fan RY,Lee RC,Teng JL,Yuen KY

    更新日期:2007-01-01 00:00:00

  • Use of pulsed-field gel electrophoresis to characterize the heterogeneity and clonality of Salmonella serotype Enteritidis, Typhimurium and Infantis isolates obtained from whole liquid eggs.

    abstract::Salmonella is a well-documented pathogen known to occur in a wide range of foods, especially poultry products. The most frequently reported food-sources of human infection are eggs and egg products. In this study, in order to describe Salmonella contamination of egg products, 144 liquid egg samples were collected from...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2008.11.021

    authors: Rivoal K,Protais J,Quéguiner S,Boscher E,Chidaine B,Rose V,Gautier M,Baron F,Grosset N,Ermel G,Salvat G

    更新日期:2009-02-15 00:00:00

  • Genetic variability and fumonisin production by Fusarium proliferatum isolated from durum wheat grains in Argentina.

    abstract::Fusarium proliferatum is a member of the Fusarium fujikuroi species complex (FFSC) involved in the maize ear rot together with Fusarium verticillioides, which is a very closely related species. Recently, different studies have detected natural fumonisin contamination in wheat kernels and most of them have shown that t...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2015.02.011

    authors: Palacios SA,Susca A,Haidukowski M,Stea G,Cendoya E,Ramírez ML,Chulze SN,Farnochi MC,Moretti A,Torres AM

    更新日期:2015-05-18 00:00:00

  • Tracking verocytotoxigenic Escherichia coli O157, O26, O111, O103 and O145 in Irish cattle.

    abstract::The purpose of this study was to investigate carriage and transfer of verocytotoxigenic Escherichia coli (VTEC) O157, O26, O111, O103 and O145 from faeces and hide to dressed carcasses of Irish cattle as well as establishing the virulence potential of VTEC carried by these cattle. Individual cattle was tracked and fae...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2011.11.012

    authors: Thomas KM,McCann MS,Collery MM,Logan A,Whyte P,McDowell DA,Duffy G

    更新日期:2012-02-15 00:00:00

  • Antimicrobial resistance in Salmonella enteritidis strains isolated from broiler carcasses, food, human and poultry-related samples.

    abstract::Antimicrobial resistance was investigated in 91 Salmonella enteritidis isolates from broiler carcasses, food, human and poultry-related samples originated from South of Brazil. A great proportion of resistant strains was found, 90.1% showing resistance to at least one antimicrobial drug. There was a high resistance to...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/j.ijfoodmicro.2004.04.022

    authors: Dias de Oliveira S,Siqueira Flores F,dos Santos LR,Brandelli A

    更新日期:2005-01-01 00:00:00

  • Reaction kinetic interpretation of heat destruction influenced by environmental factors.

    abstract::Based on reaction kinetics and thermodynamic considerations a mathematical model was developed to describe the thermal death rate of microbes as a function of environmental factors such as temperature, water activity, pH and redox potential. The mathematical model is discussed and validated by fitting to data sets of ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章

    doi:10.1016/s0168-1605(00)00474-8

    authors: Reichart O

    更新日期:2001-03-20 00:00:00