Molecular characterization of the glucose isomerase from the thermophilic bacterium Fervidobacterium gondwanense.

Abstract:

:The gene coding for xylose isomerase from the thermophilic bacterium Fervidobacterium gondwanense was cloned and overexpressed in Escherichia coli. The produced xylose isomerase (XylA), which closely resembles counterparts from Thermotoga maritima and T. neapolitana, was purified and characterized. It is optimally active at 70 degrees C, pH 7.3, with a specific activity of 15.0 U/mg for the interconversion of glucose to fructose. When compared with T. maritima XylA at 85 degrees C, a higher catalytic efficiency was observed. Divalent metal ions Co2+ and Mg2+ were found to enhance the thermostability.

journal_name

Environ Technol

journal_title

Environmental technology

authors

Kluskens LD,Zeilstra J,Geerling AC,de Vos WM,van der Oost J

doi

10.1080/09593330903486673

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

1083-90

issue

10

eissn

0959-3330

issn

1479-487X

journal_volume

31

pub_type

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