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An in vitro model of biomaterial-augmented microfracture including chondrocyte-progenitor cell interaction.

Abstract：

BACKGROUND:Biomaterials, acting as scaffolds for cell migration and differentiation, may be used to improve outcomes after microfracture. Three mechanisms determine the success of such procedures and are tested herein: the general capacity of adult femoral mesenchymal progenitor cells (MPC) to differentiate into cartilage, their capacity to do so in a biomaterial, and finally potential interactions between MPC and autologous chondrocytes. METHODS:Human adult chondrocytes and MPC were obtained with informed consent and cultured individually or in co-culture on a collagenous biomaterial. Differentiation potential of MPC was assessed using PCR and proliferation and biosynthesis were compared to test for differences between individual cultures and co-cultures. Finally, potential interaction between chondrocytes and MPC was assessed by comparing the observed levels of proliferation and biosynthesis with those expected in independent growth. RESULTS:We found that adult femoral marrow-derived MPC have the potential to differentiate into multiple lineages, and, seeded in a biomaterial, show similar differentiation when compared with autologous chondrocytes. Finally, there was a strong indication for an interaction between MPC and chondrocytes in biosynthetic activity, which was twice as high as would be expected in independent cell activity. Proliferation rates were unaffected. CONCLUSION:Our study showed that biomaterial-augmented microfracture is a viable option in cartilage repair from a biological perspective because adult femoral MPC have a strong capacity to differentiate into chondrocytes, which is further enhanced by the surrounding cartilage. Failure in in vivo studies must be explained by other factors of the intra-articular environment, such as cytokines or biomechanics.

journal_name

Arch Orthop Trauma Surg

journal_title

Archives of orthopaedic and trauma surgery

authors

Vavken P,Arrich F,Pilz M,Dorotka R

doi

10.1007/s00402-010-1079-2

subject

Has Abstract

pub_date

2010-05-01 00:00:00

pages

711-6

issue

eissn

0936-8051

issn

1434-3916

journal_volume

130

pub_type

杂志文章

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An in vitro model of biomaterial-augmented microfracture including chondrocyte-progenitor cell interaction.