Abstract:
:Severe neutropenia and protracted thrombocytopenia remain serious clinical problems following cord blood transplantation (CBT) due to the paucity of stem and progenitor cells in the grafts. Administration of ex-vivo expanded megakaryocyte progenitor cells may facilitate platelet production. We propose a novel strategy to expand these rare cells ex-vivo, from a small portion of the cord blood (CB) unit, using fibronectin (FN), a major component of hematopoietic niches, combined with cytokines, including thrombopoietin and the hematopoietic stress-associated acetylcholinesterase readthrough peptide (ARP). Application of multiple gates and high definition flow cytometry enabled clear resolution of expanded hematopoietic stem/precursor cells (HSPC) and megakaryocyte progenitors (Mk-p) and their early subsets while eliminating positively stained non-relevant cells. FN increased viability, expansion of all CD34(+) HSPC populations and Mk-p. The combination of FN + thrombopoietin + ARP maintained and expanded very early myeloid and thrombopoietic precursors, increased the proliferation of megakaryocyte, granulocyte-macrophage and multilineage colony-forming progenitors and supported Mk maturation as measured by ploidy and glycoprotein IIb/IIIa expression by quantiative reverse transcription polymerase chain reaction. This approach, which involves expanding HSPC and Mk precursors from a small portion of the CB unit, without sacrificing the coveted stem cells, may lead to improved cell therapy modalities to facilitate earlier myelopoiesis and platelet production post-CBT.
journal_name
Br J Haematoljournal_title
British journal of haematologyauthors
Deutsch V,Hubel E,Kay S,Ohayon T,Katz BZ,Many A,Zander A,Naparstek E,Grisaru Ddoi
10.1111/j.1365-2141.2009.08041.xsubject
Has Abstractpub_date
2010-04-01 00:00:00pages
137-49issue
1eissn
0007-1048issn
1365-2141pii
BJH8041journal_volume
149pub_type
杂志文章abstract::A comparison has been made between the prothrombin time test using British Comparative Thromboplastin (BCT) and a chromogenic substrate assay for factor VII in the assessment of laboratory control of oral anticoagulants in short-term and long-term patients. Opportunity was also taken to compare the findings with paral...
journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.1981.tb07198.x
更新日期:1981-09-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1046/j.1365-2141.2003.04074.x
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doi:10.1046/j.1365-2141.2000.01994.x
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.1990.tb07801.x
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abstract::To investigate the role of neutrophils in complement-induced changes in vascular permeability, skin wheal and flare responses to intradermal injection of autologous activated serum complement were measured in normal and neutropenic subjects. In normal subjects, responses were dose-dependent and were abolished by remov...
journal_title:British journal of haematology
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pub_type: 杂志文章,评审
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journal_title:British journal of haematology
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:British journal of haematology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.1985.tb07359.x
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pub_type: 杂志文章
doi:10.1111/j.1365-2141.1995.tb03386.x
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pub_type: 杂志文章
doi:10.1046/j.1365-2141.1999.01150.x
更新日期:1999-01-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:British journal of haematology
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pub_type: 杂志文章
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journal_title:British journal of haematology
pub_type: 杂志文章,多中心研究,随机对照试验
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journal_title:British journal of haematology
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journal_title:British journal of haematology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/bjh.12650
更新日期:2014-02-01 00:00:00
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journal_title:British journal of haematology
pub_type: 杂志文章
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journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1365-2141.1986.tb07507.x
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