Abstract：
BACKGROUND AND PURPOSE:The endocannabinoid, anandamide, has anti-arrhythmic effects. The aim of the present study was to explore the electrophysiological effects of anandamide on rat myocardium. EXPERIMENTAL APPROACH:Evoked action potentials (APs) were recorded using intracellular recording technique in rat cardiac papillary muscles. In addition, L-type Ca2+ current was measured and analysed using whole-cell patch-clamp recording technique in isolated rat cardiac ventricular myocytes. KEY RESULTS:In cardiac papillary muscles, anandamide (1, 10, 100 nM) decreased AP duration in a concentration-dependent manner. Furthermore, 100 nM anandamide decreased AP amplitude, overshoot and Vmax in partially depolarized papillary muscles. These effects were abolished by AM251 (100 nM), a selective antagonist for CB1 receptors, but not AM630 (100 nM), a CB2 receptor antagonist. Furthermore, an agonist of L-type Ca2+ channels, Bay K 8644 (0.5 microM), a K+ channel blocker tetraethylammonium chloride (20 mM) and the nitric oxide synthase inhibitor L-NAME (1 mM) had no effect on anandamide-induced decrease in AP duration. In isolated ventricular myocytes, anandamide (1, 10, 100 nM) decreased L-type Ca2+ current concentration-dependently, and shifted the current-voltage relationship curve of the Ca2+ current. Anandamide (100 nM) shifted the steady-state inactivation curve to the left and the recovery curve to the right. Blockade of CB1 receptors with AM251 (100 nM), but not CB2 receptors with AM630 (100 nM), eliminated the effect of anandamide on L-type Ca2+ currents. CONCLUSIONS AND IMPLICATIONS:These data suggest that anandamide suppressed AP and L-type Ca2+ current in cardiac myocytes through CB1 receptors.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Li Q,Ma HJ,Zhang H,Qi Z,Guan Y,Zhang Ydoi
10.1111/j.1476-5381.2009.00518.xsubject
Has Abstractpub_date
2009-12-01 00:00:00pages
2022-9issue
8eissn
0007-1188issn
1476-5381journal_volume
158pub_type
杂志文章abstract：:1. We assessed the relaxant effect of 17 beta-oestradiol (10(-7), 10(-6) and 10(-5) M) on rabbit isolated coronary arteries precontracted with prostaglandin F2 alpha (3 x 10(-6) M), high extracellular potassium (30 mM) and Bay K 8644 (10(-6) M) plus high extracellular potassium (15 mM) by measuring isometric tension. ...
journal_title：British journal of pharmacology
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doi：10.1111/j.1476-5381.1991.tb12545.x
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journal_title：British journal of pharmacology
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pub_type： 杂志文章,评审
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更新日期：1997-06-01 00:00:00
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journal_title：British journal of pharmacology
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doi：10.1111/j.1476-5381.1984.tb10759.x
更新日期：1984-01-01 00:00:00
