Abstract:
:1. Changes in membrane potential (measured with an intracellular microelectrode) and in cyclic nucleotide (adenosine 3':5'-cyclic monophosphate, cyclic AMP and guanosine 3':5'-cyclic monophosphate, cyclic GMP) levels (measured by radioimmunoassay) in response to inhibitory non-adrenergic non-cholinergic (NANC) field stimulation and drugs were investigated in the guinea-pig internal and anal sphincter (gpIAS) in the presence of phentolamine and atropine (each 10(-6) M). 2. Inhibitory NANC nerve stimulation (single pulse, 5 pulses at 5, 10 and 20 Hz, 0.5 ms supramaximal voltage) and adenosine triphosphate (ATP, 10(-7)-10(-3) M) inhibited spike discharge, hyperpolarized the membrane and relaxed the sphincter. The effects of inhibitory nerve stimulation were blocked by tetrodotoxin (TTX, 10(-6) M) and, with those of ATP, were blocked by apamin (5 x 10(-6) M). 3. Isoprenaline (10(-9)-10(-4) M), cromakalim (10(-9)-10(-5) M), sodium nitroprusside (NaNP 10(-5) M), M&B 22948 (10(-4) M) and 8-bromocyclic GMP (8-Br-cyclic GMP, 10(-4) M) also inhibited spike discharge, hyperpolarized the membrane and relaxed the sphincter. The effects of isoprenaline were blocked by propranolol (10(-6) M). However, forskolin (10(-9)-10(-7) M), M&B 22948 (10(-9)-10(-5) M) and lower concentrations of NaNP (10(-9)-10(-6) M) relaxed the sphincter without affecting the membrane potential. 4. The characteristics of the membrane potential changes in response to different inhibitory stimuli in the gpIAS differed. Hyperpolarization produced by inhibitory NANC nerve stimulation and ATP were rapid in onset, of brief duration and of comparable amplitude. Isoprenaline and direct electrical stimulation also hyperpolarized the membrane and relaxed the muscle although the extent of the relaxation in these two cases was much less than that with nerve stimulation and ATP. In each case, the membrane potential change preceded relaxation and probably accounted for it. 5. Both inhibitory NANC nerve stimulation (80 pulses 8Hz supramaximal voltage 0.5 ms) and ATP (10-aM) raised levels of cyclic GMP significantly and to a comparable degree and relaxed the sphincter. The effect of nerve stimulation was prevented by TTX (10- 6M) but not by apamin (5 x 10- 6M). Isoprenaline (10-s M), cromakalim (10 5 M) and forskolin (10 5M) were ineffective. 6. Inhibitory NANC nerve stimulation (80 pulses 8Hz 0.5ms supramaximal voltage) and ATP (10-4M) raised levels of cyclic AMP significantly to a comparable degree and relaxed the sphincter. The increase produced by nerve stimulation was abolished by TTX (10-6M) and apamin (5 x 10-6M). Isoprenaline (10-4M), cromakalim (10-5 M) and forskolin (10-5 M) raised levels of this nucleotide significantly.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Baird AA,Muir TCdoi
10.1111/j.1476-5381.1990.tb15804.xsubject
Has Abstractpub_date
1990-06-01 00:00:00pages
329-35issue
2eissn
0007-1188issn
1476-5381journal_volume
100pub_type
杂志文章abstract::The effects of a new selective beta1-adrenoceptor agonist, (--)-1-(4-hydroxyphenoxy)-3-isopropyl-amino-propanol-2-hydrochloride (H 133/22), on amylase secretion from the rat parotid gland were investigated in an in vitro system. The results were compared to the secretory responses obtained with noradrenaline, adrenali...
journal_title:British journal of pharmacology
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abstract:BACKGROUND AND PURPOSE:Tetracyclines were recently found to induce tumour cell death, but the early processes involved in this cytotoxic effect remain unclear. EXPERIMENTAL APPROACH:Viability of human and mouse melanoma cells was determined by MTT assay and flow cytometry. Kinase/protein/caspase activation was measure...
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journal_title:British journal of pharmacology
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abstract:BACKGROUND AND PURPOSE:Azithromycin has been reported to modify activation of macrophages towards the M2 phenotype. Here, we have sought to identify the mechanisms underlying this modulatory effect of azithromycin on human monocytes, classically activated in vitro. EXPERIMENTAL APPROACH:Human blood monocytes were prim...
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journal_title:British journal of pharmacology
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更新日期:2014-07-01 00:00:00
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更新日期:1977-10-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:1982-02-01 00:00:00
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journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1991.tb12456.x
更新日期:1991-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-03-01 00:00:00