Supraphysiologic extracellular pressure inhibits intestinal epithelial wound healing independently of luminal nutrient flow.


BACKGROUND:Luminal pressure may injure the gut mucosa in obstruction, ileus, or inflammatory bowel disease. METHODS:We formed Roux-en-Y anastomoses in 19 mice, creating proximal and defunctionalized partially obstructed limbs and a distal limb to vary luminal pressure and flow. We induced mucosal ulcers by serosal acetic acid, and assessed proliferation (proliferating cell nuclear antigen) and ERK (immunoblotting). Parallel studies compared Caco-2 enterocyte migration and proliferation after pressure and/or ERK blockade. RESULTS:At 3 days, anastomoses were probe-patent, proximal and distal limbs contained chyme, and defunctionalized limbs were empty. The proximal and defunctionalized limbs showed increased pressure and slower healing despite increased proliferation, ERK protein, and ERK activation. In vitro, pressure decreased Caco-2 migration across collagen or fibronectin, stimulated proliferation, and activated ERK. However, ERK blockade did not prevent pressure effects. CONCLUSIONS:Luminal pressure during obstruction or ileus may impair mucosal healing independently of luminal flow despite increased mitosis and ERK activation.


Am J Surg


Flanigan TL,Owen CR,Gayer C,Basson MD




Has Abstract


2008-11-01 00:00:00














  • Breast conservation surgery for breast cancer at a regional medical center.

    abstract:BACKGROUND:This study examined trends in breast conservation surgery (BCS) at our hospital and factors associated with BCS. METHODS:We retrospectively reviewed breast cancer surgeries in patients eligible for BCS (size <4 cm, N0, N1) from 1990 through 1996 (n = 634). We calculated the yearly prevalence of BCS and used...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Kotwall C,Covington D,Churchill P,Brinker C,Weintritt D,Maxwell JG

    更新日期:1998-12-01 00:00:00

  • Simplified laparoscopic parastomal hernia repair: the scroll technique.

    abstract:BACKGROUND:Laparoscopic parastomal hernia repair can be technically challenging. We herein present a simplified technique of laparoscopic parastomal hernia repair. METHODS:This technique entails fixation of the rolled mesh to the anterior abdominal wall before unfolding it, each side of the mesh is unfolded and fixed ...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Saber AA,Rao AJ,Rao CA,Elgamal MH

    更新日期:2008-09-01 00:00:00

  • A randomized, double-blind, placebo-controlled study to assess the effect of recombinant human erythropoietin on functional outcomes in anemic, critically ill, trauma subjects: the Long Term Trauma Outcomes Study.

    abstract:BACKGROUND:Achieving a higher hemoglobin (Hb) level might allow the anemic, critically ill, trauma patient to have an improved outcome during rehabilitation therapy. METHODS:Patients with major blunt trauma orthopedic injuries were administered epoetin alfa or placebo weekly both in hospital and for up to 12 weeks aft...

    journal_title:American journal of surgery

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Luchette FA,Pasquale MD,Fabian TC,Langholff WK,Wolfson M

    更新日期:2012-04-01 00:00:00

  • Short-term outcomes of splenectomy avoidance in trauma patients.

    abstract:BACKGROUND:Strategies for splenic preservation for trauma patients have gained acceptance; however, meaningful outcome evaluations have not been performed. To better understand the consequences of managing patients with splenic injuries, the short-term outcomes of different types of management strategies were examined....

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Kaseje N,Agarwal S,Burch M,Glantz A,Emhoff T,Burke P,Hirsch E

    更新日期:2008-08-01 00:00:00

  • Breast cancer in young patients.

    abstract:BACKGROUND:Breast cancer in a young person is considered by many to be a rare and very aggressive disease. Theories regarding the aggressiveness of the disease are varied. This study was undertaken in an attempt to help formulate guidelines regarding screening for and treatment of breast cancer in the young patient. P...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Sariego J,Zrada S,Byrd M,Matsumoto T

    更新日期:1995-09-01 00:00:00

  • Trauma-associated pneumonia in adult ventilated patients.

    abstract:BACKGROUND:The clinical pulmonary infection score (CPIS) and bronchoalveolar lavage (BAL) are 2 tools that have been validated to diagnose pneumonia in critically ill patients. However, the role of the CPIS in diagnosing trauma-associated pneumonia (TAP) remains in question. METHODS:This prospective observational stud...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Patel CB,Gillespie TL,Goslar PW,Sindhwani M,Petersen SR

    更新日期:2011-07-01 00:00:00

  • Open abdomen in liver transplantation.

    abstract:INTRODUCTION:Damage control laparotomy with vacuum assisted closure (VAC) is used for selective cases in trauma. In liver transplantation, VAC has also been applied for management of intra-operative hemorrhage. The primary objective was to evaluate peri-operative blood loss and blood product utilization in VAC compared...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Chan T,Bleszynski MS,Youssef DS,Segedi M,Chung S,Scudamore CH,Buczkowski AK

    更新日期:2018-05-01 00:00:00

  • Postoperative quality of life after laparoscopy-assisted pylorus-preserving gastrectomy compared With laparoscopy-assisted distal gastrectomy: A cross-sectional postal questionnaire survey.

    abstract:BACKGROUND:Little is known about postgastrectomy syndrome and quality of life (QOL after laparoscopy-assisted pylorus-preserving gastrectomy (LAPPG). The aim of this study was to assess postgastrectomy syndrome and QOL after LAPPG as compared with laparoscopy-assisted distal gastrectomy with Billroth-I reconstruction (...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Hosoda K,Yamashita K,Sakuramoto S,Katada N,Moriya H,Mieno H,Watanabe M

    更新日期:2017-04-01 00:00:00

  • Intrahepatic Glissonian approach for laparoscopic right segmental liver resections.

    abstract:BACKGROUND:Experience with laparoscopic procedures and recent advances in laparoscopic devices have created an evolving interest in the application of these techniques to liver resection. However, laparoscopic liver resection has not been widely developed and anatomical segmental liver resection is not currently perfor...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Machado MA,Makdissi FF,Galvão FH,Machado MC

    更新日期:2008-10-01 00:00:00

  • The impact of perioperative warm-up jackets on surgical site infection: cost without benefit?

    abstract:BACKGROUND:On July 1st, 2012, the University of Minnesota Medical Centers adopted a policy requiring all personnel to wear cover jackets in perioperative areas. This policy is based on the Association of Perioperative Registered Nurses recommended practice for cover jacket usage. We hypothesized that the cover jacket p...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Chow CJ,Hayes LM,Saltzman DA

    更新日期:2016-11-01 00:00:00

  • Hemorrhage after pancreaticoduodenectomy: when is surgery still indicated?

    abstract:BACKGROUND:This study analyzed presentation and management of hemorrhage after pancreaticoduodenectomy (PD) to determine the respective role of surgery and embolization. METHODS:From January 1992 to March 2005, 411 patients underwent PD and were analyzed with regard to postoperative hemorrhage. RESULTS:Hemorrhage occ...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Blanc T,Cortes A,Goere D,Sibert A,Pessaux P,Belghiti J,Sauvanet A

    更新日期:2007-07-01 00:00:00

  • Ileal intussusception secondary to small bowel metastases from melanoma.

    abstract::A 54-year-old man on palliative treatment for disseminated cutaneous malignant melanoma presented with abdominal pain and abdominal distention. A computed tomography scan showed an area in the distal ileum suggesting intussusceptions. In an exploratory laparotomy, a 10-cm mass was found comprised of the distal ileum t...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Butte JM,Meneses M,Waugh E,Parada H,De La Fuente H

    更新日期:2009-07-01 00:00:00

  • Platelet dysfunction on thromboelastogram is associated with severity of blunt traumatic brain injury.

    abstract:BACKGROUND:Platelet dysfunction associated with isolated traumatic brain injury (TBI) can be measured using thromboelastography-platelet mapping (TEG-PM). We hypothesized that platelet dysfunction can be detected after blunt TBI, and the degree of dysfunction is associated with increased TBI severity and in-hospital mo...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Kay AB,Morris DS,Collingridge DS,Majercik S

    更新日期:2019-12-01 00:00:00

  • Laparoscopic cholecystectomy under spinal anesthesia.

    abstract:BACKGROUND:Advantages of laparoscopic cholecystectomy (LC) such as less pain and short hospital stay make it the treatment of choice for cholelithiasis. There are limited data about LC under spinal anesthesia. This study was designed to evaluate LC under spinal anesthesia. METHODS:Twenty-nine patients underwent surger...

    journal_title:American journal of surgery

    pub_type: 临床试验,杂志文章


    authors: Yuksek YN,Akat AZ,Gozalan U,Daglar G,Pala Y,Canturk M,Tutuncu T,Kama NA

    更新日期:2008-04-01 00:00:00

  • Intrahepatic cholangiocarcinoma: analysis of 44 consecutive resected cases including 5 cases with repeat resections.

    abstract:BACKGROUND:Prognosis after resection for intrahepatic cholangiocarcinoma (ICC) remains unsatisfactory. There remains no effective therapy after recurrent ICC. OBJECTIVE:The current study sought to evaluate risk factors associated with recurrent ICC and possible therapies after resection. METHOD:A review of data from ...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Saiura A,Yamamoto J,Kokudo N,Koga R,Seki M,Hiki N,Yamada K,Natori T,Yamaguchi T

    更新日期:2011-02-01 00:00:00

  • Nitric oxide inhibits resting sphincter of Oddi activity.

    abstract::The sphincter of Oddi has basal myogenic phasic activity that is modulated by neural and hormonal pathways. Stimulatory innervation to this organ is cholinergic, whereas the inhibitory pathways are unknown. Nitric oxide (NO), generated from L-arginine, relaxes gastrointestinal smooth muscle in vitro. We, therefore, hy...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Kaufman HS,Shermak MA,May CA,Pitt HA,Lillemoe KD

    更新日期:1993-01-01 00:00:00

  • Impact of inappropriate initial antibiotics in critically ill surgical patients with bacteremia.

    abstract:BACKGROUND:Bloodstream infections in critically ill patients are associated with mortality as high as 60% and a prolonged hospital stay. We evaluated the impact of inappropriate antibiotic therapy (IAAT) in a critically ill surgical cohort with bacteremia. METHODS:This retrospective study evaluated adults with intensi...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Abraham K,Dolman HS,Zimmerman LH,Faris J,Edelman DA,Baylor A,Wilson RF,Tyburski JG

    更新日期:2016-03-01 00:00:00

  • Neoadjuvant chemotherapy for pancreatic cancer and changes in the biliary microbiome.

    abstract:BACKGROUND:The use of neoadjuvant chemotherapy (NAC) for pancreatic ductal adenocarcinoma (PDAC) has increased in recent years. Limited data exists on the impact of NAC on biliary microbiome. METHODS:Patients who underwent pancreaticoduodenectomy (PD) for PDAC between 2014 and 2017 were reviewed. Patients were stratif...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Nadeem SO,Jajja MR,Maxwell DW,Pouch SM,Sarmiento JM

    更新日期:2020-10-05 00:00:00

  • Second-stage pancreatojejunostomy following pancreatoduodenectomy in high-risk patients.

    abstract::Tube pancreatostomy for complete external drainage of pancreatic juice following pancreatoduodenectomy and a subsequent second-stage pancreatojejunostomy were carried out in four high-risk patients without morbidity. This procedure avoids the fatal outcome associated with pancreatojejunal anastomotic leakage following...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Miyagawa S,Makuuchi M,Kawasaki S,Ogiwara M

    更新日期:1994-07-01 00:00:00

  • Incidence, risk factors, and outcomes of perioperative acute kidney injury in noncardiac and nonvascular surgery.

    abstract:BACKGROUND:The aim of this study was to determine the incidence rate, identify the risk factors, and describe the clinical outcome of perioperative acute kidney injury (AKI) in patients undergoing noncardiac, nonvascular surgery (NCS). METHODS:A total of 1,200 adult consecutive patients undergoing NCS were prospective...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Biteker M,Dayan A,Tekkeşin Aİ,Can MM,Taycı İ,İlhan E,Şahin G

    更新日期:2014-01-01 00:00:00

  • Autologous blood transfusion does not reduce postoperative infection rates in elective surgery.

    abstract:BACKGROUND:The influence of blood transfusions in the risk of postoperative infection remains controversial. We examined the association between autologous (AB) and homologous (HB) blood transfusions with postoperative infection in elective surgery. METHODS:The medical records of 991 Medicare patients aged > or =65 ye...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Sauaia A,Alexander W,Moore EE,Stevens BR,Rosen H,Dunn TR

    更新日期:1999-12-01 00:00:00

  • Current trends and challenges in the postoperative medical management of Crohn's disease: A systematic review.

    abstract:BACKGROUND:Crohn's disease is an aggressive chronic inflammatory disorder, and despite medical advances no cure exists. There is a great risk of requiring an operative intervention, with evidence of recurrence developing in up to 80-90% of cases. Therefore, we sought to systematically review the current status in the p...

    journal_title:American journal of surgery

    pub_type: 杂志文章,评审


    authors: Schlussel AT,Cherng NB,Alavi K

    更新日期:2017-11-01 00:00:00

  • Small bowel transit and gastric emptying after biliodigestive anastomosis using the uncut jejunal loop.

    abstract:BACKGROUND:The Roux-en-Y loop is an effective procedure for biliodigestive drainage. However, up to 15% of patients suffer from postoperative cholangitis or blind loop syndrome. A new technique to prevent motility abnormalities has been developed. METHODS:Male Lewis rats were used to compare gastric emptying and trans...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Klaus A,Hinder RA,Nguyen JH,Nelson KL

    更新日期:2003-12-01 00:00:00

  • Debt and other influences on career choices among surgical and primary care residents in a community-based hospital system.

    abstract:BACKGROUND:To evaluate debt and other factors that help formulate the career paths of future surgical and primary care physicians, a survey was undertaken. METHODS:Surgical specialty (SS) and primary care (PC) residents were surveyed regarding demographics, factors influencing choice of specialty, methods of financing...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Woodworth PA,Chang FC,Helmer SD

    更新日期:2000-12-01 00:00:00

  • Balloon versus patch angioplasty as an adjuvant treatment to surgical thrombectomy of hemodialysis grafts.

    abstract:BACKGROUND:Transluminal balloon angioplasty offers advantages to patch angioplasty. We evaluated the primary patency of thrombosed hemodialysis grafts that had undergone balloon angioplasty versus patch angioplasty as a salvage method. METHODS:We reviewed our experience with 22 consecutive intraoperative balloon angio...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Bitar G,Yang S,Badosa F

    更新日期:1997-08-01 00:00:00

  • Pediatric intestinal obstruction in Malawi: characteristics and outcomes.

    abstract:BACKGROUND:Intestinal obstruction (IO) is a common pediatric surgical emergency in sub-Saharan Africa with high morbidity and mortality, but little is known about its etiopathogenesis in Malawi. METHODS:Retrospective analysis of patients seen from February 2012 to June 2014 at Kamuzu Central Hospital in Lilongwe, Mala...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Shah M,Gallaher J,Msiska N,McLean SE,Charles AG

    更新日期:2016-04-01 00:00:00

  • Immunotherapy of cancer with "immune" RNA. A preliminary report.

    abstract::A phase I clinical trial of immunotherapy with "Immune" RNA was undertaken fifteen months ago. Twenty-six cancer patients were treated with RNA extracted from the lymphoid organs of sheep immunized with either autologous tumor cells or allogeneic tumor cells of the same histologic type. Eighteen patients had gross dis...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: Pilch YH,de Kernion JB,Skinner DG,Ramming KP,Schick PM,Fritze D,Brower P,Kern DH

    更新日期:1976-11-01 00:00:00

  • Utility of fluorine-18-fluorodeoxyglucose positron emission tomography in differentiated thyroid carcinoma with negative radioiodine scans and elevated serum thyroglobulin levels.

    abstract:BACKGROUND:This study aimed to determine the role of fluorine-18-fluorodeoxyglucose positron emission tomography (FDG-PET) in the follow-up of patients who underwent total thyroidectomy and iodine-131 ((131)I) ablation therapy for differentiated thyroid cancer and presented increased thyroglobulin levels with negative ...

    journal_title:American journal of surgery

    pub_type: 临床试验,杂志文章


    authors: Muros MA,Llamas-Elvira JM,Ramírez-Navarro A,Gómez MJ,Rodríguez-Fernández A,Muros T,López de la Torre M,Becerra A,Carreras JL

    更新日期:2000-06-01 00:00:00

  • Use of ultrasound to demonstrate gallstones in symptomatic patients with normal oral cholecystograms.

    abstract::Seven patients who had typical and persistent symptoms of gallbladder disease but repeatedly normal oral cholecystograms were seen in an 18 month period. Ultrasound examination of the gallbladder was diagnostic of cholelithiasis in all seven patients. Six patients were found to have gallstones at operation, and the se...

    journal_title:American journal of surgery

    pub_type: 杂志文章


    authors: McCluskey PL,Prinz RA,Guico R,Greenlee HB

    更新日期:1979-11-01 00:00:00

  • An Internet-based survey of factors influencing medical student selection of a general surgery career.

    abstract:BACKGROUND:This study descriptively analyzes characteristics of general surgery residency and practice and their influence on student interest in surgical careers. METHODS:Fourth-year medical students were invited to complete an Internet-based survey. A 5-point Likert scale described characteristics of general surgery...

    journal_title:American journal of surgery

    pub_type: 杂志文章,多中心研究


    authors: Cochran A,Melby S,Neumayer LA

    更新日期:2005-06-01 00:00:00