Neurokinin-2 receptor levels correlate with intensity, frequency, and duration of pain in chronic pancreatitis.

Abstract:

OBJECTIVE:Generation and maintenance of pain in chronic pancreatitis (CP) have been shown to be partially attributable to neuroimmune interactions, which involve neuropeptides such as substance P (SP). So far, expression of SP receptors NK-2R, NK-3R, the SP-encoding gene preprotachykinin A (PPT-A), and the SP degradation enzyme neutral endopeptidase (NEP) and their relation to pain in CP have not been determined. METHODS:Tissue samples from patients with CP (n = 25) and from healthy donors (n = 20) were analyzed for PPT-A, NK-2R, NK-3R, and NEP expression using quantitative RT-PCR. NEP protein levels were examined by immunoblot analysis and its localization was determined using immunohistochemistry. A scoring system was used to grade the extent of fibrosis on hematoxylin and eosin- and Masson-Trichrome-stained sections. Messenger RNA levels and the extent of pain were analyzed for correlations. RESULTS:In CP tissues, NK-2R and PPT-A expression was increased, whereas NK-3R and NEP mRNA levels were comparable with normal pancreas. Overexpression of NK-2R was related to the intensity, frequency, and duration of pain in CP patients. NK-1R and NEP expression was significantly related to the extent of fibrosis. CONCLUSIONS:Expression of NK-2R and PPT-A is increased in CP and is associated with pain. Failure to up-regulate NEP may contribute to the disruption of the neuropeptides loop balance in CP and thus may exacerbate the severe pain syndrome.

journal_name

Ann Surg

journal_title

Annals of surgery

authors

Michalski CW,Shi X,Reiser C,Fachinger P,Zimmermann A,Büchler MW,Di Sebastiano P,Friess H

doi

10.1097/SLA.0b013e318070d56e

subject

Has Abstract

pub_date

2007-11-01 00:00:00

pages

786-93

issue

5

eissn

0003-4932

issn

1528-1140

pii

00000658-200711000-00015

journal_volume

246

pub_type

杂志文章
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    更新日期:2009-08-01 00:00:00

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    更新日期:2011-06-01 00:00:00

  • Significant contribution of the portal vein to blood flow through the common bile duct.

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    更新日期:2012-03-01 00:00:00

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    journal_title:Annals of surgery

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    authors: Wherry DC,Rob CG,Marohn MR,Rich NM

    更新日期:1994-11-01 00:00:00

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    authors: Salloum RM,Copeland EM,Souba WW

    更新日期:1991-05-01 00:00:00

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    更新日期:2008-01-01 00:00:00

  • Administration of balanced or BCAA-enriched amino acid solution in septic rats. Effects on protein synthesis in the liver.

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    pub_type: 杂志文章

    doi:10.1097/00000658-198812000-00007

    authors: Pedersen P,Li SJ,Hasselgren PO,LaFrance R,Fischer JE

    更新日期:1988-12-01 00:00:00

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198309000-00003

    authors: Leicher FG,Magrassi P,LaRaia PJ,Derkac WM,Buckley MJ,Austen WG

    更新日期:1983-09-01 00:00:00

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198403000-00008

    authors: Arbeit JM,Lees DE,Corsey R,Brennan MF

    更新日期:1984-03-01 00:00:00

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    pub_type: 杂志文章

    doi:10.1097/00000658-198008000-00011

    authors: Leckie PA,Ferreira P,Debas HT

    更新日期:1980-08-01 00:00:00

  • An Implementation Assessment of the Virtual Acute Care for Elders Program From the Perspective of Key Stakeholders.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000004433

    authors: Balentine CJ,Morris M,Knight SJ,Turan JM,Flood KL,Gutierrez-Meza D,Macdonald CL,Bhatia S,Brown CJ

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    journal_title:Annals of surgery

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00000658-197602000-00009

    authors: Shanik GD,Ford J,Hayes AC,Baker WH,Barnes RW

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    authors: Dixon E,Vollmer CM Jr,Sahajpal A,Cattral M,Grant D,Doig C,Hemming A,Taylor B,Langer B,Greig P,Gallinger S

    更新日期:2005-03-01 00:00:00

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-197708000-00018

    authors: Perloff LJ,Rowlands DT,Barker CF

    更新日期:1977-08-01 00:00:00

  • Colon surgery for cancer in the very elderly. Cost and 3-year survival.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-198602000-00003

    authors: Hobler KE

    更新日期:1986-02-01 00:00:00

  • Cost-efficacy of cultured epidermal autografts in massive pediatric burns.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/00000658-200006000-00011

    authors: Barret JP,Wolf SE,Desai MH,Herndon DN

    更新日期:2000-06-01 00:00:00

  • HuR status is a powerful marker for prognosis and response to gemcitabine-based chemotherapy for resected pancreatic ductal adenocarcinoma patients.

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0b013e3181f1fd44

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    pub_type: 杂志文章,多中心研究

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    journal_title:Annals of surgery

    pub_type: 杂志文章,评审

    doi:10.1097/00000658-198703000-00013

    authors: Sumpio BE,Jennings TA,Merino MJ,Sullivan PD

    更新日期:1987-03-01 00:00:00

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    journal_title:Annals of surgery

    pub_type: 临床试验,杂志文章,随机对照试验

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    更新日期:2005-09-01 00:00:00

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    pub_type: 杂志文章

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    更新日期:1976-04-01 00:00:00

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    pub_type: 杂志文章

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    pub_type: 杂志文章,评审

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    pub_type: 杂志文章

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    pub_type: 杂志文章

    doi:10.1097/00000658-198006000-00006

    authors: Flint LM Jr,McCoy M,Richardson JD,Polk HC Jr

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    journal_title:Annals of surgery

    pub_type: 杂志文章

    doi:10.1097/SLA.0000000000001651

    authors: Leff DR,Yongue G,Vlaev I,Orihuela-Espina F,James D,Taylor MJ,Athanasiou T,Dolan R,Yang GZ,Darzi A

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    更新日期:2006-09-01 00:00:00