Decreased neutrophil response in a model of chronic intraperitoneal Klebsiella infection.

Abstract:

BACKGROUND:Previously, we developed an infection model by intraperitoneal (IP) injection of Klebsiella pneumoniae. The high early mortality rate prompted a study of the effect of gentamicin on the disease course. METHODS:Infection was induced in Balb/c mice by IP inoculation of 10(3) colony-forming units (cfu) of K. pneumoniae serotype 2. Mice then received either saline or gentamicin twice daily at 5 mg/kg/day. Survival and weight loss were determined daily over 14 days. Leukocyte counts (WBC) were performed on peritoneal lavage fluid (PL) and peripheral blood. Bacterial counts in blood, lung homogenate, and PL were determined by culture. RESULTS:A significant survival benefit was seen in the gentamicin group by 48 h (p < 0.05), which persisted at 14 days. Weight loss of 2 g (p < 0.05) occurred in all mice by day three, with recovery seen only in the gentamicin-treated mice. Mice that did not regain their weight by day seven or lost more than 4 g (20%) died. All animals had peritoneal bacteria recovered at all time points. The gentamicin group showed fewer bacteria at one day in lung, blood, and PL, the difference being significant for the PL. Gentamicin did not clear peritoneal bacteria, and counts remained at inoculum concentration (10(3) cfu), but elicited no significant neutrophil influx. Whereas there was a progressive increase in total lavage WBC over time, the gentamicin group showed no significant neutrophil influx in PL or lung until bacterial counts exceeded 10(5) cfu. CONCLUSIONS:Despite persistent intra-abdominal infection, gentamicin treatment significantly prolonged survival in this uniformly lethal model. Although antibiotic treatment did not suppress bacteremia, it did diminish bacterial spread from the blood to the lung. This procedure produced a clinically relevant, novel model of antibiotic-treated chronic intraperitoneal infection, which will allow future study of specific host defense mechanisms.

journal_name

Surg Infect (Larchmt)

journal_title

Surgical infections

authors

Woods DR,Peyton JC,Franklin GA,Turina M,Cheadle WG

doi

10.1089/sur.2006.060

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

367-76

issue

3

eissn

1096-2964

issn

1557-8674

journal_volume

8

pub_type

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