Abstract:
:LINE-1 elements comprise approximately 17% of human DNA and their mobility continues to impact genome evolution. However, little is known about the types of non-transformed cells that can support LINE-1 retrotransposition. Here, we show that human embryonic stem cells express endogenous LINE-1 elements and can accommodate LINE-1 retrotransposition in vitro. The resultant retrotransposition events can occur into genes and can result in the concomitant deletion of genomic DNA at the target site. Thus, these data suggest that LINE-1 retrotransposition events may occur during early stages of human development.
journal_name
Hum Mol Genetjournal_title
Human molecular geneticsauthors
Garcia-Perez JL,Marchetto MC,Muotri AR,Coufal NG,Gage FH,O'Shea KS,Moran JVdoi
10.1093/hmg/ddm105subject
Has Abstractpub_date
2007-07-01 00:00:00pages
1569-77issue
13eissn
0964-6906issn
1460-2083pii
ddm105journal_volume
16pub_type
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