Abstract:
:We describe a new Type II congenital disorder of glycosylation (CDG-II) caused by mutations in the conserved oligomeric Golgi (COG) complex gene, COG8. The patient has severe psychomotor retardation, seizures, failure to thrive and intolerance to wheat and dairy products. Analysis of serum transferrin and total serum N-glycans showed normal addition of one sialic acid, but severe deficiency in subsequent sialylation of mostly normal N-glycans. Patient fibroblasts were deficient in sialylation of both N- and O-glycans, and also showed slower brefeldin A (BFA)-induced disruption of the Golgi matrix, reminiscent of COG7-deficient cells. Patient fibroblasts completely lacked COG8 protein and had reduced levels and/or mislocalization of several other COG proteins. The patient had two COG8 mutations which severely truncated the protein and destabilized the COG complex. The first, IVS3 + 1G > A, altered the conserved splicing site of intron 3, and the second deleted two nucleotides (1687-1688 del TT) in exon 5, truncating the last 47 amino acids. Lentiviral-mediated complementation with normal COG8 corrected mislocalization of other COG proteins, normalized sialylation and restored normal BFA-induced Golgi disruption. We propose to call this new disorder CDG-IIh or CDG-II/COG8.
journal_name
Hum Mol Genetjournal_title
Human molecular geneticsauthors
Kranz C,Ng BG,Sun L,Sharma V,Eklund EA,Miura Y,Ungar D,Lupashin V,Winkel RD,Cipollo JF,Costello CE,Loh E,Hong W,Freeze HHdoi
10.1093/hmg/ddm028subject
Has Abstractpub_date
2007-04-01 00:00:00pages
731-41issue
7eissn
0964-6906issn
1460-2083pii
ddm028journal_volume
16pub_type
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