Abstract：
:A simple spectroscopic filtering technique is presented that may aid the assignment of (13)C and (15)N resonances of methyl-containing amino-acids in solid-state magic-angle spinning (MAS) NMR. A filtering block that selects methyl resonances is introduced in two-dimensional (2D) (13)C-homonuclear and (15)N-(13)C heteronuclear correlation experiments. The 2D (13)C-(13)C correlation spectra are recorded with the methyl filter implemented prior to a (13)C-(13)C mixing step. It is shown that these methyl-filtered (13)C-homonuclear correlation spectra are instrumental in the assignment of C(delta) resonances of leucines by suppression of C(gamma)-C(delta) cross peaks. Further, a methyl filter is implemented prior to a (15)N-(13)C transferred-echo double resonance (TEDOR) exchange scheme to obtain 2D (15)N-(13)C heteronuclear correlation spectra. These experiments provide correlations between methyl groups and backbone amides. Some of the observed sequential (15)N-(13)C correlations form the basis for initial sequence-specific assignments of backbone signals of the outer-membrane protein G.
journal_name
J Biomol NMRjournal_title
Journal of biomolecular NMRauthors
Jehle S,Hiller M,Rehbein K,Diehl A,Oschkinat H,van Rossum BJdoi
10.1007/s10858-006-9078-xsubject
Has Abstractpub_date
2006-11-01 00:00:00pages
169-77issue
3eissn
0925-2738issn
1573-5001journal_volume
36pub_type
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journal_title：Journal of biomolecular NMR
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