Abstract:
:Airway hyper-reactivity to inhaled adenosine, mediated via mast cell activation, is a cardinal feature of asthma. Animal models have been developed in several species to mimic this phenomenon, but only in the rat has a mast cell involvement been clearly defined. In this study, a model of ovalbumin-induced adenosine hyper-reactivity was developed in BALB/c mice to determine whether mast cells are involved in this phenomenon. Sensitised mice were challenged one, two or three times, on a daily basis, and airway responses to the stable adenosine analogue NECA (5'-N-ethylcarboxamido adenosine) determined 4 and 24 h after each challenge. Airway hyper-reactivity was observed in ovalbumin-challenged mice 4 h after a single challenge and to a minor extent 24 h after a single challenge and 4 h after two challenges. Cromolyn (20 mg ml(-1)), given by aerosol an hour before the NECA provocation, fully inhibited the airway hyper-reactivity observed 4 h after a single allergen challenge, suggesting a role for mast cells in this response. The airway space cellular inflammation was not affected by cromolyn. As observed in human asthma, an acute treatment with steroid (budesonide 3 mg kg(-1), given an hour before the allergen challenge) inhibited the NECA airway hyper-reactivity and significantly inhibited the airway space cellular inflammation. These data suggest that the ovalbumin-challenged BALB/c mice can be considered as a suitable model to study the adenosine-induced airway hyper-reactivity phenomenon observed in human asthma.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Wyss D,Bonneau O,Trifilieff Adoi
10.1038/sj.bjp.0706271subject
Has Abstractpub_date
2005-08-01 00:00:00pages
845-52issue
7eissn
0007-1188issn
1476-5381pii
0706271journal_volume
145pub_type
杂志文章abstract::1 The relative potencies of methotrimeprazine, (+)-methotrimeprazine, (+/-)-10-(3-dimethylamino-2-methylpropyl)-2-valeroyl phenothiazine hydrochloride (M & B 18,706) and (+)-M & B 18,706 in reducing the pressor action of noradrenaline in the spinal cat, reducing intercollicular decerebrate rigidity, and muscle spindle...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1974.tb09610.x
更新日期:1974-03-01 00:00:00
abstract::1. Neurogenic responses to transmural electrical stimulation were examined in endothelium-denuded extrameningeal (vertebral and carotid) and intrameningeal (spinal, basilar and middle cerebral) arteries isolated from dogs. 2. In the extrameningeal arteries, transmural electrical stimulation produced a phasic contracti...
journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1995.tb17207.x
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journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1983.tb08807.x
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abstract::1. The effect of central injection of selective kinin B1 and B2 receptor antagonists on the febrile response induced by endotoxin (E. coli lipopolysaccharide, LPS) in rats was investigated. 2. Intracerebroventricular (i.c.v.) injection of a selective B2 receptor antagonist (Hoe-140, 8 nmol) reduced the early (0-2 h), ...
journal_title:British journal of pharmacology
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doi:10.1038/sj.bjp.0701110
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journal_title:British journal of pharmacology
pub_type: 杂志文章,评审
doi:10.1038/bjp.2008.147
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journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1980.tb10696.x
更新日期:1980-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjp.0704517
更新日期:2002-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1476-5381.1994.tb13110.x
更新日期:1994-06-01 00:00:00
abstract::1. A wide panel of compounds acting on beta-adrenoceptors active either in mammalian heart or in rodent digestive tract and adipose tissues, were investigated for their effects on Chinese hamster ovary cells transfected with the human or murine beta 3-adrenoceptor gene. 2. The beta 3-agonists, bucindolol, CGP 12177A a...
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pub_type: 杂志文章
doi:10.1111/j.1476-5381.1994.tb17116.x
更新日期:1994-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1996.tb15460.x
更新日期:1996-06-01 00:00:00
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doi:10.1111/j.1476-5381.1970.tb09924.x
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更新日期:2014-03-01 00:00:00
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更新日期:1994-04-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2012-03-01 00:00:00
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更新日期:2013-07-01 00:00:00
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更新日期:1983-01-01 00:00:00
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更新日期:1993-09-01 00:00:00