当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY期刊下所有文献 > Real-time PCR-based methods for detection of Mycobacterium avium subsp. paratuberculosis in water and milk.

Real-time PCR-based methods for detection of Mycobacterium avium subsp. paratuberculosis in water and milk.

Abstract：

:A real-time PCR assay for quantitative detection of Mycobacterium avium paratuberculosis has been developed. It targets and amplifies sequences from the IS900 insertion element which is specific for this bacterium, and includes an internal amplification control. The assay was tested against 18 isolates of M. avium paratuberculosis, 17 other mycobacterial strains, and 25 non-mycobacterial strains, and was fully selective. It is capable of detecting <3 genomic DNA copies with 99% probability or alternatively, using cells directly in the reaction, 12 cells can be detected with 99% probability. Using prior centrifugation, the assay was able to consistently and quantifiably detect 10(2) M. avium paratuberculosis cells in 20 ml artificially contaminated drinking water. With a simple detergent and enzymatic sample pretreatment before centrifugation and nucleic acid extraction, the assay was able to consistently detect 10(2) M. avium paratuberculosis in 20 ml artificially contaminated semi-skimmed milk. The assay will be a useful addition to the range of diagnostic tools available for the study of M. avium paratuberculosis.

journal_name

Int J Food Microbiol

journal_title

International journal of food microbiology

authors

Rodríguez-Lázaro D,D'Agostino M,Herrewegh A,Pla M,Cook N,Ikonomopoulos J

doi

10.1016/j.ijfoodmicro.2004.09.005

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

93-104

issue

1

eissn

0168-1605

issn

1879-3460

pii

S0168-1605(04)00534-3

journal_volume

101

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY文献大全
  • Biodiversity and probiotic potential of yeasts isolated from Fura, a West African spontaneously fermented cereal.

    abstract：:Fura is a spontaneously fermented pearl millet product consumed in West Africa. The yeast species involved in the fermentation were identified by pheno- and genotypic methods to be Candida krusei, Kluyveromyces marxianus, Candida tropicalis, Candida rugosa, Candida fabianii, Candida norvegensis and Trichosporon asahii...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2012.08.016

    authors： Pedersen LL,Owusu-Kwarteng J,Thorsen L,Jespersen L

    更新日期：2012-10-01 00:00:00

  • Adhesion of Salmonella typhimurium var. copenhagen in the intestines of pigeons.

    abstract：:The attachment of Salmonella typhimurium var. copenhagen to the intestinal epithelium of pigeons was studied. After experimental infection, the intestines of both young and adult pigeons were examined in vivo and in vitro. Investigations were carried out by electron and immunofluorescence microscopy. Attachment of the...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/0168-1605(92)90062-8

    authors： Grund S,Stolpe H

    更新日期：1992-03-01 00:00:00

  • The antimicrobial effects of cinnamon leaf oil against multi-drug resistant Salmonella Newport on organic leafy greens.

    abstract：:There is generally no kill-step when preparing salad vegetables, so there is a greater risk for foodborne illness from contaminated vegetables. Some essential oils have antimicrobial activities and could provide a natural way to reduce pathogens on fresh produce. The objective of this study was to investigate the anti...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2013.06.021

    authors： Todd J,Friedman M,Patel J,Jaroni D,Ravishankar S

    更新日期：2013-08-16 00:00:00

  • Quantification of the impact of single and multiple mild stresses on outgrowth heterogeneity of Bacillus cereus spores.

    abstract：:Outgrowth heterogeneity of bacterial spore populations complicates both prediction and efficient control of spore outgrowth. In this study, the impact of mild preservation stresses on outgrowth of Bacillus cereus ATCC 14579 spores was quantified during the first stages of outgrowth. Heterogeneity in outgrowth of heat-...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2014.02.015

    authors： van Melis CC,den Besten HM,Nierop Groot MN,Abee T

    更新日期：2014-05-02 00:00:00

  • Further data on the production of beauvericin, enniatins and fusaproliferin and toxicity to Artemia salina by Fusarium species of Gibberella fujikuroi species complex.

    abstract：:The knowledge of toxigenic profiles of fungal plant pathogens is of extreme importance for evaluating the potential toxicity of infected plant products. Ninety-six fungal isolates belonging to 28 species in the Gibberella fujikuroi complex were studied for the production of beauvericin, enniatins and fusaproliferin in...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2007.07.004

    authors： Moretti A,Mulè G,Ritieni A,Logrieco A

    更新日期：2007-09-15 00:00:00

  • Investigations on the pathogenicity of Listeria spp. by experimental infection of the chick embryo.

    abstract：:To investigate the pathogenicity of Listeria spp., chick embryos were infected by two methods. Very encouraging results were obtained with the inoculation of the chorioallantoic membrane (CAM) of 10 day old chick embryos: embryos inoculated with pathogenic strains (L. monocytogenes or L. ivanovii) died within 72 h, wh...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/0168-1605(89)90027-5

    authors： Terplan G,Steinmeyer S

    更新日期：1989-06-01 00:00:00

  • Antimicrobial effect of spices and herbs on Vibrio parahaemolyticus.

    abstract：:The antimicrobial effects of spices and herbs from 18 plant species were examined on a foodborne pathogen, Vibrio parahaemolyticus, with the use of combinations of temperatures and nutrient levels. Basil, clove, garlic, horseradish, marjoram, oregano, rosemary, and thyme exhibited antibacterial activities at incubatio...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2006.04.031

    authors： Yano Y,Satomi M,Oikawa H

    更新日期：2006-08-15 00:00:00

  • Growth and mycotoxin production by fungi in atmospheres containing 80% carbon dioxide and 20% oxygen.

    abstract：:The effect of atmosphere containing 80% CO(2) and 20% O(2) on growth of Mucor plumbeus, Fusarium oxysporum, Byssochlamys fulva, Byssochlamys nivea, Penicillium commune, Penicillium roqueforti, Aspergillus flavus, Eurotium chevalieri and Xeromyces bisporus was investigated. Production of aflatoxin by A. flavus, patulin...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2010.08.030

    authors： Taniwaki MH,Hocking AD,Pitt JI,Fleet GH

    更新日期：2010-10-15 00:00:00

  • Detection of different microenvironments and Lactobacillus sakei biotypes in Ventricina, a traditional fermented sausage from central Italy.

    abstract：:The present study evaluated the physico-chemical and microbiological features of Ventricina, considering for the first time the presence of different compartments deriving from the technology of production. In fact meat pieces (pork muscle and fat cut into cubes of about 10-20cm3), mixed with other ingredients and the...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2016.11.009

    authors： Tremonte P,Sorrentino E,Pannella G,Tipaldi L,Sturchio M,Masucci A,Maiuro L,Coppola R,Succi M

    更新日期：2017-02-02 00:00:00

  • Detection of hepatitis E virus RNA in raw sausages and liver sausages from retail in Germany using an optimized method.

    abstract：:Hepatitis E virus (HEV) is a pathogen of increasing importance, which can be zoonotically transmitted from domestic pigs, wild boar, and deer to humans. Foodborne transmission by consumption of raw and undercooked liver, meat, or sausages prepared from infected animals has been documented. The aim of this study was to...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2015.09.013

    authors： Szabo K,Trojnar E,Anheyer-Behmenburg H,Binder A,Schotte U,Ellerbroek L,Klein G,Johne R

    更新日期：2015-12-23 00:00:00

  • Occurrence of plasmids and tetracycline resistance among Campylobacter jejuni and Campylobacter coli isolated from whole market chickens and clinical samples.

    abstract：:Twenty whole market chickens, purchased from 10 different stores in the Taipei Metropolitan area, were examined for the presence of Campylobacter jejuni and Campylobacter coli. The microorganisms were recovered from 95% of the chickens. A survey of different sites on--breast, thigh and tail--showed that contamination ...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/0168-1605(94)90115-5

    authors： Lee CY,Tai CL,Lin SC,Chen YT

    更新日期：1994-12-01 00:00:00

  • Study of surface yeast flora of Roquefort cheese.

    abstract：:The change in yeast flora on the surface of two batches of Roquefort cheese was monitored over a period of 6 months. 401 isolates were determined and their technological properties were investigated. The main species isolated were: Debaryomyces hansenii and its non sporulating form Candida famata, Kluyveromyces lactis...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/0168-1605(92)90014-t

    authors： Besançon X,Smet C,Chabalier C,Rivemale M,Reverbel JP,Ratomahenina R,Galzy P

    更新日期：1992-09-01 00:00:00

  • Multidrug resistant clones of Salmonella Infantis of broiler origin in Europe.

    abstract：:The aim of the study was to investigate the potential spread of the previously described multidrug-resistant (MDR) Hungarian clone of Salmonella Infantis of broiler origin in Europe. Therefore, 76 strains isolated between 2004 and 2009 from raw meat and fecal samples of broiler origin in nine European countries - incl...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2012.04.007

    authors： Nógrády N,Király M,Davies R,Nagy B

    更新日期：2012-06-15 00:00:00

  • Genetic and serological identification of three Vibrio parahaemolyticus strains as candidates for novel provisional O serotypes.

    abstract：:Vibrio parahaemolyticus is a Gram-negative, halophilic Vibrio that naturally inhabits marine and estuarine environments worldwide and has recently been recognized as one of the most important foodborne pathogens. To date, 13 O serotypes and 71 K serotypes of V. parahaemolyticus have been identified. However, untypeabl...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2017.01.010

    authors： Guo X,Liu B,Chen M,Wang Y,Wang L,Chen H,Wang Y,Tu L,Zhang X,Feng L

    更新日期：2017-03-20 00:00:00

  • Oleic acid and ergosterol supplementation mitigates oxidative stress in wine strains of Saccharomyces cerevisiae.

    abstract：:During fermentation of high-sugar-containing medium lacking lipid nutrients, wine yeasts undergo oxidative stress and oxidative damage to cell membranes and proteins. Considering that cell membranes are important stress sensors, and that under hypoxic conditions wine yeasts modulate cell membranes composition by incor...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2010.05.020

    authors： Landolfo S,Zara G,Zara S,Budroni M,Ciani M,Mannazzu I

    更新日期：2010-07-15 00:00:00

  • Quantifying strain variability in modeling growth of Listeria monocytogenes.

    abstract：:Prediction of microbial growth kinetics can differ from the actual behavior of the target microorganisms. In the present study, the impact of strain variability on maximum specific growth rate (μmax) (h(-1)) was quantified using twenty Listeria monocytogenes strains. The μmax was determined as function of four differe...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2015.05.006

    authors： Aryani DC,den Besten HM,Hazeleger WC,Zwietering MH

    更新日期：2015-09-02 00:00:00

  • Evaluation of high pressure processing (HPP) inactivation of Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in acid and acidified juices and beverages.

    abstract：:Increasing consumer demand for high-quality foods has driven adoption by the food industry of non-thermal technologies such as high pressure processing (HPP). The technology is employed as a post-packaging treatment step for inactivation of vegetative microorganisms. In order to evaluate HPP inactivation of Escherichi...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2020.109034

    authors： Usaga J,Acosta Ó,Churey JJ,Padilla-Zakour OI,Worobo RW

    更新日期：2020-12-31 00:00:00

  • Relevance of microbial interactions to predictive microbiology.

    abstract：:Microbial interaction can be ignored in predictive microbiology under most conditions. We show that interactions are only important at high population densities, using published data on inhibition of growth of Listeria monocytogenes in broth. Our analysis using growth models from predictive microbiology indicated that...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/s0168-1605(02)00424-5

    authors： Malakar PK,Barker GC,Zwietering MH,van't Riet K

    更新日期：2003-08-01 00:00:00

  • Some examples of, and some problems with, the use of nonlinear logistic regression in predictive food microbiology.

    abstract：:A new technique, nonlinear logistic regression, is described for modelling binomially distributed data, i.e., presence/absence data where growth is either observed or not observed, for applications in predictive food microbiology. Some examples of the successful use of this technique are presented, where the controlli...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/s0168-1605(01)00643-2

    authors： Ratkowsky DA

    更新日期：2002-03-01 00:00:00

  • Costs of acute bacterial foodborne disease in Canada and the United States.

    abstract：:Bacterial foodborne disease is increasing in industrialized as well as developing countries. For Canada and the United States many millions of cases are believed to occur each year, based on extrapolations of survey data, human enteric isolations and reported foodborne disease cases. The economic impact of such a larg...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章,评审

    doi：10.1016/0168-1605(89)90099-8

    authors： Todd EC

    更新日期：1989-12-01 00:00:00

  • Viability of microencapsulated bifidobacteria in simulated gastric juice and bile solution.

    abstract：:Microencapsulated cells of Bifidobacterium longum B6 and Bifidobacterium infantis CCRC 14633 were prepared by spray drying the cell suspension containing the test organism and 10% (w/w) of the carrier material of either gelatin, soluble starch, skim milk or gum arabic. Survival of these microencapsulated and free cell...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/s0168-1605(02)00563-9

    authors： Lian WC,Hsiao HC,Chou CC

    更新日期：2003-09-15 00:00:00

  • A quantitative exposure model simulating human norovirus transmission during preparation of deli sandwiches.

    abstract：:Human noroviruses (HuNoVs) are a major cause of food borne gastroenteritis worldwide. They are often transmitted via infected and shedding food handlers manipulating foods such as deli sandwiches. The presented study aimed to simulate HuNoV transmission during the preparation of deli sandwiches in a sandwich bar. A qu...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2014.12.004

    authors： Stals A,Jacxsens L,Baert L,Van Coillie E,Uyttendaele M

    更新日期：2015-03-02 00:00:00

  • Spoilage of blood sausages morcilla de Burgos treated with high hydrostatic pressure.

    abstract：:In this study, the microbial ecology of the blood sausage morcilla de Burgos, subjected to high hydrostatic pressure treatment (HPP), was studied by culture-dependent and -independent methods. Morcilla de Burgos is the most traditional and famous blood sausage in Spain. The producers are interested in extending its sh...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2008.02.017

    authors： Diez AM,Urso R,Rantsiou K,Jaime I,Rovira J,Cocolin L

    更新日期：2008-04-30 00:00:00

  • Development of an affordable typing method for Meyerozyma guilliermondii using microsatellite markers.

    abstract：:Despite previously published methods, there is still a lack of rapid and affordable methods for genotyping the Meyerozyma guilliermondii yeast species. The development of microsatellite markers is a useful genotyping method in several yeast species. Using the Tandem Repeat Finder Software, a total of 19 microsatellite...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2015.10.008

    authors： Wrent P,Rivas EM,Peinado JM,de Silóniz MI

    更新日期：2016-01-18 00:00:00

  • Taking injuries of surviving bacteria into account for optimising heat treatments.

    abstract：:The main assets of early conventional models applied in the field of canned food industries are their simplicity and their robustness. Moreover, a certain standardisation of these models allows the intrinsic quantification of a food process like sterilisation, regardless of the nature of concerned microbial population...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1605(00)00160-4

    authors： Mafart P

    更新日期：2000-04-10 00:00:00

  • Effectiveness of lemon juice, vinegar and their mixture in the elimination of Salmonella typhimurium on carrots (Daucus carota L.).

    abstract：:Lemon juice, vinegar and the mixture of lemon juice and vinegar (1:1) were tested for their effectiveness in reducing the counts of inoculated Salmonella typhimurium (approximately 6 and 3 log cfu/g) on carrots. Treatment of carrot samples with lemon juice vinegar alone for different exposure times (0, 15, 30 and 60 m...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2004.04.010

    authors： Sengun IY,Karapinar M

    更新日期：2004-11-15 00:00:00

  • Discrimination of infectious and heat-treated norovirus by combining platinum compounds and real-time RT-PCR.

    abstract：:Human noroviruses (NoV) are major agents of foodborne outbreaks. Because of the lack of a standardized cell culture method, real-time reverse transcriptase PCR is now commonly used for the detection of NoV in foodstuffs and environmental samples. However, this approach detects the viral nucleic acids of both infectiou...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2018.01.015

    authors： Fraisse A,Niveau F,Hennechart-Collette C,Coudray-Meunier C,Martin-Latil S,Perelle S

    更新日期：2018-03-23 00:00:00

  • Inhibitory effect of four novel synthetic peptides on food spoilage yeasts.

    abstract：:The spoilage of foods caused by the growth of undesirable yeast species is a problem in the food industry. Yeast species such as Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Debaryomyces hansenii, Kluyveromyces lactis and Saccharomyces cerevisiae have been encountered in foods such as high sugar products, fruit...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2019.04.005

    authors： Shwaiki LN,Arendt EK,Lynch KM,Thery TLC

    更新日期：2019-07-02 00:00:00

  • Microbiological safety of traditional and starter-mediated processes for the manufacture of Italian dry sausage.

    abstract：:Microbiological changes occurring during the commercial manufacture of Italian dry sausages (Genoa and salametti) were studied in two urban Canadian centres over a 5 month period. A comparison was made between 6 plants which used bacterial starter cultures and 4 plants where more traditional processes (without starter...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/0168-1605(88)90072-4

    authors： Holley RA,Lammerding AM,Tittiger F

    更新日期：1988-08-01 00:00:00

  • Tracking of Listeria monocytogenes in meat establishment using Whole Genome Sequencing as a food safety management tool: A proof of concept.

    abstract：:Repeated Listeria outbreaks particularly associated with Ready-To-Eat (RTE) delicatessen meat products have been reported annually at global level. The most frequent scenario that led to foodborne outbreaks was the post-thermal treatment cross-contamination of deli meat products during slicing and modified atmosphere ...

    journal_title：International journal of food microbiology

    pub_type： 杂志文章

    doi：10.1016/j.ijfoodmicro.2017.06.015

    authors： Nastasijevic I,Milanov D,Velebit B,Djordjevic V,Swift C,Painset A,Lakicevic B

    更新日期：2017-09-18 00:00:00