Effect of vascular endothelial growth factor and epidermal growth factor on iatrogenic apoptosis in human endothelial cells.


:To study the effect of growth factors on iatrogenic apoptosis, we examined the influence of vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) on staurosporine-induced apoptosis in primary cultures of human umbilical vein endothelial cells (HUVEC). Apoptosis was evaluated by a cell viability test, the TUNEL-POD assay and the activation of the pro-apoptotic caspase-3. Staurosporine (10-100nM) caused the activation of caspase-3. This effect was manifest after 2hr of incubation and reached its maximum after 5hr. Severe loss of viability followed within 18hr. VEGF or EGF (10-100ng/mL) added together with staurosporine decreased the activation of caspase-3. The loss of viability was 24hr delayed. The action of growth factors was observed at 1% serum concentration but also at concentration optimal for HUVEC survival (10%, v/v). Furthermore, the inhibition of PI-3 kinase (PI-3K) by wortmannin or LY294002 as well as the inhibition of MEK by PD098059 or U0126 prevented the protective effect of VEGF and EGF. Western blotting analysis showed that after 3hr of incubation with staurosporine the level of the anti-apoptotic protein Mcl-1 decreased and this effect was reverted by VEGF. It is concluded that VEGF and EGF antagonize the pro-apoptotic action of staurosporine by the combined signalling of PI-3K and ERKs pathways.


Biochem Pharmacol


Biochemical pharmacology


Vinci MC,Visentin B,Cusinato F,Nardelli GB,Trevisi L,Luciani S




Has Abstract


2004-01-15 00:00:00














  • Cerium-induced strain-dependent increase in Cyp2a-4/5 (cytochrome P4502a-4/5) expression in the liver and kidneys of inbred mice.

    abstract::The murine Cyp2a-4 and Cyp2a-5 genes encode P450 isoforms catalysing testosterone 15 alpha-hydroxylase and coumarin 7-hydroxylase (COH) activities, respectively. Two days after the administration of a hepatotoxic dose of cerium chloride (2 mg/kg i.v.), COH activity was increased 3.2-fold in the liver of DBA/2 mice. Th...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Salonpää P,Iscan M,Pasanen M,Arvela P,Pelkonen O,Raunio H

    更新日期:1992-10-06 00:00:00

  • The role of cytochrome P450 and cytochrome P450 reductase in the reductive bioactivation of the novel benzotriazine di-N-oxide hypoxic cytotoxin 3-amino-1,2,4-benzotriazine-1,4-dioxide (SR 4233, WIN 59075) by mouse liver.

    abstract::SR 4233 or WIN 59075 (3-amino-1,2,4-benzotriazine-1,4-dioxide) is a novel and highly selective hypoxic cell cytotoxin requiring reductive bioactivation for its impressive antitumour effects. Expression of appropriate reductases will contribute to therapeutic selectivity. Here we provide more detailed information on th...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Walton MI,Wolf CR,Workman P

    更新日期:1992-07-22 00:00:00

  • Exploiting the balance between life and death: targeted cancer therapy and "oncogenic shock".

    abstract::Rational approaches to targeted cancer therapy have begun to predominate the pipelines of oncology drug development. Our rapidly increasing understanding of the "wiring" of tumor cells and the vulnerabilities of such cells that can potentially be exploited through targeted treatments has opened up enormous opportuniti...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Sharma SV,Settleman J

    更新日期:2010-09-01 00:00:00

  • Cross-talk between minimally primed HL-60 cells and resting HUVEC reveals a crucial role for adhesion over extracellularly released oxidants.

    abstract::This study demonstrates that a long-lasting co-culture of neutrophil surrogates (HL-60 cells), minimally primed by platelet activating factor (PAF), and resting endothelial cells (EC) results in the elaboration of an hyper-adhesive endothelial surface, as measured by the increase in the expression of endothelial adhes...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Allegra M,D'Acquisto F,Tesoriere L,Livrea MA,Perretti M

    更新日期:2011-02-01 00:00:00

  • Correlation between ribosomal RNA production and RNA-directed fluoropyrimidine cytotoxicity.

    abstract::The relationship between cytotoxicity and fluoropyrimidine effects on the production of mature cytoplasmic 28S and 18S ribosomal RNA was studied in S-180 cells for the fluoropyrimidines: 5-fluorouracil (FUra), 5-fluorouridine (FUrd), 5-fluorodeoxyuridine (FdUrd), and 5'-deoxy-5-fluorouridine (5'-dFUrd). After a 6-hr d...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Takimoto CH,Tan YY,Cadman EC,Armstrong RD

    更新日期:1987-10-01 00:00:00

  • Parkin is transcriptionally regulated by the aryl hydrocarbon receptor: Impact on α-synuclein protein levels.

    abstract::Parkin (PRKN) is a ubiquitin E3 ligase that catalyzes the ubiquitination of several proteins. Mutations in the human Parkin gene, PRKN, leads to degeneration of dopaminergic (DA) neurons, resulting in autosomal recessive early-onset parkinsonism and the loss of PRKN function is linked to sporadic Parkinson's disease (...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: González-Barbosa E,García-Aguilar R,Vega L,Cabañas-Cortés MA,Gonzalez FJ,Segovia J,Morales-Lázaro SL,Cisneros B,Elizondo G

    更新日期:2019-10-01 00:00:00

  • SARM: From immune regulator to cell executioner.

    abstract::SARM is the fifth and most conserved member of the Toll/Il-1 Receptor (TIR) adaptor family. However, unlike the other TIR adaptors, MyD88, Mal, TRIF and TRAM, SARM does not participate in transducing signals downstream of TLRs. By contrast SARM inhibits TLR signalling by interacting with the adaptors TRIF and MyD88. I...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Carty M,Bowie AG

    更新日期:2019-03-01 00:00:00

  • The measurement of polyglutamate metabolites of the thymidylate synthase inhibitor, ICI D1694, in mouse and human cultured cells.

    abstract::A method is described for the measurement of the polyglutamates of the quinazoline thymidylate synthase inhibitor, N-(5-[N-(3,4-dihydro-2-methyl-4-oxoquinazolin- 6-ylmethyl)-N-methylamino]-2-theonyl)-L-glutamic acid (ICI D1694). This involved incubation of cells with [5-3H]ICI D1694, extraction of the polyglutamates a...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Gibson W,Bisset GM,Marsham PR,Kelland LR,Judson IR,Jackman AL

    更新日期:1993-02-24 00:00:00

  • Zinc protoporphyrin suppresses cancer cell viability through a heme oxygenase-1-independent mechanism: the involvement of the Wnt/β-catenin signaling pathway.

    abstract::Zinc protoporphyrin (ZnPP), a known inhibitor of heme oxygenase-1 (HO-1), has been reported to have anticancer activity in both in vitro and in vivo model systems. While the mechanisms of ZnPP's anticancer activity remain to be elucidated, it is generally believed that ZnPP suppresses tumor growth through inhibition o...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Wang S,Avery JE,Hannafon BN,Lind SE,Ding WQ

    更新日期:2013-06-01 00:00:00

  • Acetylcholinesterase prophylaxis against organophosphate poisoning. Quantitative correlation between protection and blood-enzyme level in mice.

    abstract::Fetal bovine serum acetylcholinesterase (FBS-AChE, EC was titrated, both in vitro and in vivo, with a highly toxic anti-ChE organophosphate, 7-(methylethoxyphosphinyloxy)-1-methyl-quinolinium iodie (MEPQ). Approximately 1:1 stoichiometry was obtained for the sequestration of MEPQ by FBS-AChE in mice. A quanti...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Raveh L,Ashani Y,Levy D,De La Hoz D,Wolfe AD,Doctor BP

    更新日期:1989-02-01 00:00:00

  • Opioid-like compound exerts anti-fibrotic activity via decreased hepatic stellate cell activation and inflammation.

    abstract::Hepatic fibrosis is characterized by excess type I collagen deposition and exacerbated inflammatory response. Naltrexone, an opioid receptor antagonist used for treating alcohol abuse, attenuates hepatocellular injury in fibrotic animal models, which can be accompanied by deleterious side effects. Additionally, opioid...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Day SA,Lakner AM,Moore CC,Yen MH,Clemens MG,Wu ES,Schrum LW

    更新日期:2011-04-15 00:00:00

  • Anti-fibrotic and anti-inflammatory properties of melatonin on human gingival fibroblasts in vitro.

    abstract::Melatonin (MEL) has been proposed as a therapeutic agent for the oral cavity, due to its antioxidant and anti-inflammatory effects since periodontal diseases are aggravated by free radicals, and by disproportionate immunological response to plaque microorganism. In addition, MEL promotes bone formation. This study aim...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Gómez-Florit M,Ramis JM,Monjo M

    更新日期:2013-12-15 00:00:00

  • TRPV4: new therapeutic target for inflammatory bowel diseases.

    abstract::The transient receptor potential vanilloid-4 (TRPV4) belongs to a family of ion channels and can be activated by warm temperature, hypotonicity, cell swelling or lipid mediators of the arachidonic cascade. The metabolites or events responsible for TRPV4 activation are associated with inflammation, arguing in favor of ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Vergnolle N

    更新日期:2014-05-15 00:00:00

  • Impaired receptor-mediated endocytosis by the asialoglycoprotein receptor in ethanol-fed mice: implications for studying the role of this receptor in alcoholic apoptosis.

    abstract::During receptor-mediated endocytosis (RME), extracellular molecules are internalized after being recognized and bound to specific cell surface receptors. In previous studies of the asialoglycoprotein receptor (ASGPR) in rats, we showed that ethanol impairs RME at multiple ASGPR sites. Ethanol administration has been s...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Dalton SR,Wiegert RL,Baldwin CR,Kassel KM,Casey CA

    更新日期:2003-02-15 00:00:00

  • GABAA receptor modulation by piperine and a non-TRPV1 activating derivative.

    abstract::The action of piperine (the pungent component of pepper) and its derivative SCT-66 ((2E,4E)-5-(1,3-benzodioxol-5-yl))-N,N-diisobutyl-2,4-pentadienamide) on different gamma-aminobutyric acid (GABA) type A (GABA(A)) receptors, transient-receptor-potential-vanilloid-1 (TRPV1) receptors and behavioural effects were invest...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Khom S,Strommer B,Schöffmann A,Hintersteiner J,Baburin I,Erker T,Schwarz T,Schwarzer C,Zaugg J,Hamburger M,Hering S

    更新日期:2013-06-15 00:00:00

  • Inactivation of bee venom phospholipase A2 by a sesquiterpene furanoic acid marine natural product.

    abstract::A sesquiterpene furanoic acid (SFA) marine natural product isolated from soft corals of the genus Sinularia (Bowden et al., Aust J Chem 36: 371-376, 1983) was found to inactivate bee venom phospholipase A2 (bvPLA2, EC in vitro. In this study, we characterized the kinetics of inactivation of bvPLA2 by this com...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Grace KJ,Zavortink D,Jacobs RS

    更新日期:1994-04-20 00:00:00

  • Analysis of C-terminally substituted tachykinin-like peptide agonists by means of aequorin-based luminescent assays for human and insect neurokinin receptors.

    abstract::Aequorin-based assays for stable fly, Stomoxys calcitrans, (STKR) and human (neurokinin receptor 1 (NK1), neurokinin receptor 2 (NK2)) neurokinin-like receptors were employed to investigate the impact of a C-terminal amino acid exchange in synthetic vertebrate ('FXGLMa') and invertebrate ('FX1GX2Ra') tachykinin-like p...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Torfs H,Detheux M,Oonk HB,Akerman KE,Poels J,Van Loy T,De Loof A,Vassart G,Parmentier M,Vanden Broeck J

    更新日期:2002-05-01 00:00:00

  • Malvidin-3-O-β glucoside, major grape anthocyanin, inhibits human macrophage-derived inflammatory mediators and decreases clinical scores in arthritic rats.

    abstract::Polyphenolic anthocyanins are major colorful compounds in red fruits, known to prevent cardiovascular and other diseases. Grape polyphenols are a mixture of various molecules and their exact contribution to above bioactivities remains to be clarified. In the present study, we first analyzed the effect of purified grap...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Decendit A,Mamani-Matsuda M,Aumont V,Waffo-Teguo P,Moynet D,Boniface K,Richard E,Krisa S,Rambert J,Mérillon JM,Mossalayi MD

    更新日期:2013-11-15 00:00:00

  • TGF-beta in cancer and as a therapeutic target.

    abstract::Cancer develops through a series of genetic changes leading to malignant transformation. Numerous gene and pathways involved in stages of progression to frank malignancy have been elucidated. These genetic changes result in aberrations in fundamental cellular processes controlling proliferation, apoptosis, differentia...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Pinkas J,Teicher BA

    更新日期:2006-08-28 00:00:00

  • The interaction of oral hypoglycaemic drugs with insulin on steroid metabolism in hepatocytes isolated from control and diabetic male rats.

    abstract::The effects of the oral hypoglycaemic drugs, phenformin and tolbutamide, and insulin, alone and in combination, on steroid metabolism in hepatocytes isolated from control and streptozotocin-diabetic male rats has been studied. Both phenformin and tolbutamide mimic the action of insulin in stimulating hepatic steroid m...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Hussin AH,Skett P

    更新日期:1990-11-15 00:00:00

  • Iron complexes of deferiprone and dietary plant catechols as cytoprotective superoxide radical scavengers(1).

    abstract::Superoxide radicals have been implicated in the pathogenesis of aging, cataract, ischemia-reperfusion, cancer and inflammatory diseases. In the present work, we found that deferiprone (L1), an iron-chelating drug, and dietary dihydroxycinnamic acids (catechols) were much more effective at protecting isolated rat hepat...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Moridani MY,O'Brien PJ

    更新日期:2001-12-15 00:00:00

  • The interactions of apamin and tetraethylammonium are differentially affected by single mutations in the pore mouth of small conductance calcium-activated potassium (SK) channels.

    abstract::Valine residues in the pore region of SK2 (V366) and SK3 (V520) were replaced by either an alanine or a phenylalanine to evaluate the impact on the interactions with the allosteric blocker apamin. Unlike TEA which showed high sensitivity to phenylalanine mutated channels, the binding affinity of apamin to the phenylal...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Dilly S,Philippart F,Lamy C,Poncin S,Snyders D,Seutin V,Liégeois JF

    更新日期:2013-02-15 00:00:00

  • Flow linear dichroism supports an accordion model for the salt-induced condensation of chromatin.

    abstract::The salt-induced condensation of chromatin has been studied with flow-linear dichroism technique using an intercalative dye (methylene blue) to selectively monitor the linker orientation. At low ionic strength both linkers and chromatosomes (with their flat faces) are oriented preferentially parallel to the chromatin ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Kubista M,Nielsen PE,Nordén B

    更新日期:1988-05-01 00:00:00

  • Pharmacological and biochemical determinants of the antitumour activity of the indoloquinone EO9.

    abstract::EO9 is a novel bioreductive drug which has recently undergone extensive clinical evaluation. Its mechanism of action remains to be clearly defined. Antitumour activity of EO9 has been determined in 2 human colon cancer xenografts (HT-29 and BE) and 2 murine colon adenocarcinomas (MAC 16 and 26) after intratumoural inj...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Cummings J,Spanswick VJ,Gardiner J,Ritchie A,Smyth JF

    更新日期:1998-02-01 00:00:00

  • Gs alpha availability to cholera toxin-catalysed ADP-ribosylation is decreased in membranes of retinoic acid-treated leukemic cell lines HL-60 and THP-1. A posttranslational effect.

    abstract::Retinoic acid (RA) induces HL-60 and THP-1 leukemic cell lines to differentiate into granulocyte-like and monocyte-like cells. Limited data are available concerning the effects of RA on components of the cyclic AMP pathway in human myeloid leukemic cells. We showed previously a decrease in adenylate cyclase activity i...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: de Cremoux P,Zimber A,Calvo F,Lanotte M,Mercken L,Abita JP

    更新日期:1991-11-06 00:00:00

  • Tetradecanoyl phorbol-13-acetate counteracts the responsiveness of cultured thyroid cells to thyrotropin.

    abstract::We have studied the effects of TPA on the metabolism of porcine thyroid cells cultured for 1-4 days in the absence (control cells) and in the presence of 0.1 mU/ml TSH (TSH cells). The phospholipid turnover, evaluated after a 2 hr incorporation of 32P-phosphate into phospholipids, is markedly modified by the presence ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Haye B,Aublin JL,Champion S,Lambert B,Jacquemin C

    更新日期:1985-11-01 00:00:00

  • Diabetes-induced down-regulation of beta1-adrenoceptor mRNA expression in rat heart.

    abstract::The present study addressed the question of whether the number of myocardial beta-adrenoceptors in rats with 4- to 6-week streptozotocin-induced diabetes is regulated in a transcriptional or translational manner. Radioligand binding experiments with [3H]CGP 12177 {4-(3-t-butylamino-2-hydroxypropoxy)-[5,7-3H]benzimidaz...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Matsuda N,Hattori Y,Gando S,Akaishi Y,Kemmotsu O,Kanno M

    更新日期:1999-09-01 00:00:00

  • Effects of membrane depolarization and changes in intra- and extracellular calcium concentration on phosphoinositide hydrolysis in bovine tracheal smooth muscle.

    abstract::Agonist-stimulated phosphoinositide metabolism plays a central role in pharmacomechanical coupling in airways smooth muscle (ASM). In many other tissues and cells, most noteably excitable cells, membrane depolarization or an increase in intracellular Ca2+ ([Ca2+]i) generated by inositol 1,4,5-trisphosphate (Ins(1,4,5)...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Chilvers ER,Lynch BJ,Offer GJ,Challiss RA

    更新日期:1994-06-15 00:00:00

  • Interaction of streptomycin and streptomycylamine derivatives with negatively charged lipid layers. Correlation between binding, conformation of complexes and inhibition of lysosomal phospholipase activities.

    abstract::Aminoglycoside antibiotics induce a lysosomal phospholipidosis in kidney proximal tubules after conventional therapy in animals and man. We have previously demonstrated that these drugs bind to negatively charged phospholipid bilayers at acid pH and inhibit the activity of lysosomal acid phospholipases in vitro and in...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Brasseur R,Carlier MB,Laurent G,Claes PJ,Vanderhaeghe HJ,Tulkens PM,Ruysschaert JM

    更新日期:1985-04-01 00:00:00

  • Role of sodium in intracellular calcium elevation and leukotriene B4 formation by receptor-mediated activation of human neutrophils.

    abstract::The role of Na(+) and Na(+) exchangers in intracellular Ca(2+) elevation and leukotriene B(4) (LTBs) formation was investigated in granulocyte macrophage colony-stimulating factor (GM-CSF)-primed, fMLP-stimulated human neutrophils. Isotonic substitution of extracellular Na(+) with N-methyl-D-glucamine(+) (NMDG(+)) res...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Tedeschi A,Ciceri P,Zarini S,Lorini M,Di Donato M,Nicosia S,Miadonna A,Sala A

    更新日期:2004-01-15 00:00:00