Expression of cyclooxygenase-1 and -2 in human breast cancer.

Abstract:

PURPOSE:We investigated the expression of cyclooxygenase (Cox-1 and Cox-2) in mammary tissues from patients with breast cancer. METHODS:We used reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. RESULTS:The cancer cells showed very weak expression of Cox-1, but strong expression of immunoreactive Cox-2. In contrast, immunoreactive Cox-2 was very weak in all of the benign mammary tumors examined, including fibroadenoma (FA) and mastopathy (MP). Immunoreactive Cox-1 was also very weak in these benign tumors. The extent and intensity of immunoreactive Cox-2 polypeptides was significantly greater in the cancer cells than in the FA cells or MP cells. RT-PCR analysis showed enhanced expression of Cox-2, but not Cox-1 in breast cancer tissue, and faint expression of Cox-2 in benign tissue. CONCLUSIONS:These results demonstrated that human breast cancer cells generated Cox-2, indicating that Cox-2 might play an important role in the proliferation of breast cancer cells.

journal_name

Surg Today

journal_title

Surgery today

authors

Yoshimura N,Sano H,Okamoto M,Akioka K,Ushigome H,Kadotani Y,Yoshimura R,Nobori S,Higuchi A,Ohmori Y,Nakamura K

doi

10.1007/s00595-003-2606-3

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

805-11

issue

11

eissn

0941-1291

issn

1436-2813

journal_volume

33

pub_type

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