Abstract:
:A machine that employs a novel reagent delivery technique for biomolecular synthesis has been developed. This machine separates the addressing of individual synthesis sites from the actual process of reagent delivery by using masks placed over the sites. Because of this separation, this machine is both cost-effective and scalable, and thus the time required to synthesize 384 or 1536 unique biomolecules is very nearly the same. Importantly, the mask design allows scaling of the number of synthesis sites without the addition of new valving. Physical and biological comparisons between DNA made on a commercially available synthesizer and this unit show that it produces DNA of similar quality.
journal_name
Genome Resjournal_title
Genome researchauthors
Livesay EA,Liu YH,Luebke KJ,Irick J,Belosludtsev Y,Rayner S,Balog R,Johnston SAdoi
10.1101/gr.359002subject
Has Abstractpub_date
2002-12-01 00:00:00pages
1950-60issue
12eissn
1088-9051issn
1549-5469journal_volume
12pub_type
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