Abstract:
:The higher-order structural organization and dynamics of the chromosomes play a central role in gene regulation. To explore this structure-function relationship, it is necessary to directly visualize genomic elements in living cells. Genome imaging based on the CRISPR system is a powerful approach but has limited applicability due to background signals and nonspecific aggregation of fluorophores within nuclei. To address this issue, we developed a novel visualization scheme combining tripartite fluorescent proteins with the SunTag system and demonstrated that it strongly suppressed background fluorescence and amplified locus-specific signals, allowing long-term tracking of genomic loci. We integrated the multicomponent CRISPR system into stable cell lines to allow quantitative and reliable analysis of dynamic behaviors of genomic loci. Due to the greatly elevated signal-to-background ratio, target loci with only small numbers of sequence repeats could be successfully tracked, even under a conventional fluorescence microscope. This feature enables the application of CRISPR-based imaging to loci throughout the genome and opens up new possibilities for the study of nuclear processes in living cells.
journal_name
Genome Resjournal_title
Genome researchauthors
Chaudhary N,Nho SH,Cho H,Gantumur N,Ra JS,Myung K,Kim Hdoi
10.1101/gr.260018.119subject
Has Abstractpub_date
2020-09-01 00:00:00pages
1306-1316issue
9eissn
1088-9051issn
1549-5469pii
gr.260018.119journal_volume
30pub_type
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