Activation time course of activator protein-1 and effect of proline dithiocarbamate during ischemia-reperfusion in rat skeletal muscle.

Abstract:

:Activator protein 1 (AP-1) is thought to play an important role in the expression of genes expressed in response to ischemia-reperfusion injury. In this report, the activation of AP-1 in rat skeletal muscle during reperfusion after a 4-hour ischemic period was studied. AP-1 activation displayed a biphasic pattern, showing peak activities at 1 hour after perfusion and from 4 hours to 12 hours after perfusion. Inhibition of AP-1 activation was investigated using a potent nuclear factor kappa B inhibitor, proline dithiocarbamate (Pro-DTC). AP-1 binding activity at 1 hour of reperfusion was significantly reduced (29.0 +/- 10.1% SEM; p < 0.05) after intravenous administration of Pro-DTC (n = 7 animals in each group). Further elucidation of the role of AP-1 is warranted in hopes of developing strategies to reduce the deleterious effects of ischemia-reperfusion injury.

journal_name

Ann Plast Surg

authors

Lefler SR,Lille ST,Huemer GM,Tucker R,Murray T,Schoeller T,Mulligan DC

doi

10.1097/00000637-200212000-00016

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

654-9

issue

6

eissn

0148-7043

issn

1536-3708

pii

00000637-200212000-00016

journal_volume

49

pub_type

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