Abstract:
:Apoptosis at the site of rupture has been proposed to play a role in premature rupture of the fetal membranes, a condition associated with increased risk of neonatal sepsis and preterm birth. We investigated the ability of peroxisome proliferator-activated receptor (PPAR)-gamma ligands 15-deoxy-delta12,14PGJ2 (15d-PGJ2), delta12PGJ2, ciglitizone and rosiglitazone to induce apoptosis in the amnion-like WISH cell line. 15d-PGJ2 (10 microM) induced morphological characteristics of apoptosis within 2 h, with biochemical indices (caspase activation and substrate cleavage) following shortly after; maximum cell death (approximately 60%) was observed by 16 h, with an EC50) of approximately 7 microM 15d-PGJ2. Delta12-PGJ2 also induced apoptosis but was less potent and acted at a much slower rate. While ciglitizone also induced apoptosis, rosiglitazone had no effect on cell viability. The mechanism of induction of apoptosis by 15d-PGJ2 and delta12PGJ2, which may be independent of PPAR-gamma activation, requires further elucidation.
journal_name
Prostaglandins Other Lipid Mediatjournal_title
Prostaglandins & other lipid mediatorsauthors
Keelan J,Helliwell R,Nijmeijer B,Berry E,Sato T,Marvin K,Mitchell M,Gilmour Rdoi
10.1016/s0090-6980(01)00164-2subject
Has Abstractpub_date
2001-12-01 00:00:00pages
265-82issue
4eissn
1098-8823pii
S0090-6980(01)00164-2journal_volume
66pub_type
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