Abstract：
:1. The endothelial modulation of the relaxant responses to the nitric oxide (NO) donor sodium nitroprusside (SNP) and the KATP channel opener levcromakalim (LEM) and the interactions between these agents were analysed in isolated rat aorta. 2. LEM-induced relaxation was unchanged by endothelium removal or by the presence of L-NAME (10-4 M) or ODQ (10-6 M). In contrast, in KCl- (25 mM), but not in noradrenaline- (NA, 10-6 M) contracted arteries, SNP-induced relaxation was augmented by endothelium removal but not by L-NAME, indomethacin, glibenclamide nor charybdotoxin plus apamin. 3. The isobolographic analysis of the interactions between exogenously activated KATP channels and cyclic GMP using mixtures of SNP and LEM revealed that there were no interactions between both drugs at the proportions at which both drugs were active. However, the points for the SNP : LEM mixtures in proportions 10:1 and 1:10,000 (i.e. at concentrations at which LEM and SNP were inactive, respectively) fell significantly above the line of additivity indicating that there were negative interactions between both drugs at these selected proportions (about 5- and 2 fold inhibition, respectively). The former interaction was sensitive to glibenclamide, whereas the latter was insensitive ODQ. The magnitude of the 10:1 SNP:LEM interaction was smaller in endothelium-intact arteries and was absent in arteries stimulated by NA. 4. In conclusion, the relaxations induced by LEM and SNP were additive. However, the presence of endothelium and low concentrations of LEM inhibited SNP-induced relaxation. Both inhibitory effects were not additive and were only observed in KCl- and not in NA-contracted aortae.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Pérez-Vizcaíno F,Cogolludo AL,Zaragozá-Arnáez F,Fajardo S,Ibarra M,López-López JG,Tamargo Jdoi
10.1038/sj.bjp.0702924subject
Has Abstractpub_date
1999-12-01 00:00:00pages
1419-26issue
7eissn
0007-1188issn
1476-5381journal_volume
128pub_type
杂志文章abstract：:1. Isolated preparations of rat anococcygeus muscle were incubated with [3H]-noradrenaline and the efflux of radioactivity induced by stimulation of intramural sympathetic nerves was used as a measure of release of transmitter noradrenaline. Isometric contractile responses were also measured. 2. Angiotensin I (0.03 mi...
journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1988.tb11657.x
更新日期：1988-10-01 00:00:00
abstract：:1 The effects of pretreatment with aspirin, and of prostacyclin (PGI(2)) infusions, on responses to myocardial ischaemia and infarction produced by ligation of a coronary artery were investigated in conscious rats.2 Surgical preparation, under halothane anaesthesia, consisted of implanting exteriorized aortic and jugu...
journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1983.tb09359.x
更新日期：1983-01-01 00:00:00
abstract：:We investigated the rate of penetration into and the intra-relationship between the serum, cerebrospinal fluid (CSF) and regional brain extracellular fluid (bECF) compartments following systemic administration of lamotrigine in rat. The serum pharmacokinetics were biphasic with an initial distribution phase, (half-lif...
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doi：10.1038/sj.bjp.0703337
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1976.tb07696.x
更新日期：1976-09-01 00:00:00
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journal_title：British journal of pharmacology
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doi：10.1038/sj.bjp.0702361
更新日期：1999-02-01 00:00:00
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journal_title：British journal of pharmacology
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doi：10.1038/sj.bjp.0702132
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1992.tb14345.x
更新日期：1992-06-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
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更新日期：1972-11-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
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journal_title：British journal of pharmacology
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更新日期：1985-04-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1038/sj.bjp.0705049
更新日期：2003-02-01 00:00:00
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journal_title：British journal of pharmacology
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更新日期：2000-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1476-5381.1996.tb15749.x
更新日期：1996-11-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1979.tb17333.x
更新日期：1979-01-01 00:00:00
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doi：10.1111/j.1476-5381.1984.tb10814.x
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更新日期：2001-08-01 00:00:00
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更新日期：2007-10-01 00:00:00
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doi：10.1111/j.1476-5381.1977.tb07536.x
更新日期：1977-08-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章,评审
doi：10.1111/bph.12353
更新日期：2014-02-01 00:00:00
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更新日期：1998-10-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/bph.13121
更新日期：2015-06-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1974.tb09651.x
更新日期：1974-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：1974-12-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1981.tb10428.x
更新日期：1981-06-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1972.tb07236.x
更新日期：1972-01-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1994.tb17074.x
更新日期：1994-11-01 00:00:00
abstract：:1. Under normal experimental conditions, the rabbit isolated distal saphenous artery appears to contain a homogeneous population of postjunctional alpha 1-adrenoceptors. Prazosin competitively antagonized responses to noradrenaline (NA) with a pA2 value of 8.6, while a relatively high concentration of rauwolscine (1 m...
journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1991.tb09815.x
更新日期：1991-06-01 00:00:00
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journal_title：British journal of pharmacology
pub_type： 杂志文章
doi：10.1111/j.1476-5381.1986.tb10186.x
更新日期：1986-03-01 00:00:00
