Effects of osmotic pressure and brain-derived neurotrophic factor on the survival of postnatal hypothalamic oxytocinergic and vasopressinergic neurons in dissociated cell culture.

Abstract:

:Neurons from hypothalamic paraventricular nuclei (PVN) and supraoptic nuclei (SON) from postnatal day 6-8 rats were enzymatically dissociated and separately maintained in monolayer cultures for 14 days. The osmotic pressure of the culture medium, based on Neurobasal medium (Life Technologies), was varied (255, 300 and 330 mOsm/l) by adjustment using mannitol. The survival of oxytocin (OT), vasopressin (VP) and oxytocin-vasopressin (OT/VP) coexpressing neurons were studied under these varied conditions, and the identification of the cell phenotypes in the cultures was carried out by using double-label immunofluorescence. Under control osmolar conditions (300 mOsm/l) equivalent numbers of OT and VP neurons were found in the SON (P = 0.8398) and PVN (P = 0.4721) cultures. The OT neurons' survival did not change in 255 or 330 mOsm media in the SON cultures, but the VP neurons in the SON cultures were significantly increased in 255 mOsm/l medium as compared to control (300 mOsm/l) medium (P = 0.0088). No significant changes were found in VP neuron survival in SON cultures between the 300-330 mOsm/l media (P = 0.2372). Similar data were obtained for the VP neurons in PVN-derived cultures, but the OT neurons in these cultures survived significantly better at 300 mOs/l than at 255 mOsm/l (P<0.0001), but were not significantly different at 330 mOsm/l (P = 0.1208). In general, the VP neurons were more vulnerable than OT neurons to increases of culture medium osmolarity with respect to their survival. The number of OT/VP coexpressing neurons was greater in SON-derived cell cultures as compared to PVN-derived cell cultures, and their numbers were higher in the lower osmolarity media. The effects of adding brain-derived neurotrophic factor (BDNF) to the culture medium on survival were determined. BDNF significantly increased the numbers of all three types of neurons in both PVN and SON cell cultures (P = 0.0001-0.0060). The phenotypically identified cells, cultured in the 300 mOsm/l medium, responded by depolarization or hyperpolarization when transferred to hypertonic or hypotonic perfusion salines, respectively.

journal_name

J Neuroendocrinol

authors

Kusano K,House SB,Gainer H

doi

10.1046/j.1365-2826.1999.00296.x

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

145-52

issue

2

eissn

0953-8194

issn

1365-2826

journal_volume

11

pub_type

杂志文章
  • Introduction of a high-energy diet acutely up-regulates hypothalamic cocaine and amphetamine-regulated transcript, Mc4R and brown adipose tissue uncoupling protein-1 gene expression in male Sprague-Dawley rats.

    abstract::Obesity is an escalating problem in Western societies. Susceptibility to weight gain within an obesogenic environment is variable. It remains unclear how the range of weight gain responses are generated. It is possible that an individual's immediate and/or sustained appetite for apparently palatable foods, or metaboli...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2005.01269.x

    authors: Archer ZA,Rayner DV,Duncan JS,Bell LM,Mercer JG

    更新日期:2005-01-01 00:00:00

  • Effects of central administration of resistin on renal sympathetic nerve activity in rats fed a high-fat diet: a comparison with leptin.

    abstract::Similar to leptin, resistin acts centrally to increase renal sympathetic nerve activity (RSNA). In high-fat fed animals, the sympatho-excitatory effects of leptin are retained, in contrast to the reduced actions of leptin on dietary intake. In the present study, we investigated whether the sympatho-excitatory actions ...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/jne.12495

    authors: Habeeballah H,Alsuhaymi N,Stebbing MJ,Badoer E

    更新日期:2017-08-01 00:00:00

  • A tale of two rhythms: the emerging roles of oxytocin in rhythmic prolactin release.

    abstract::Hormone secretion often occurs in a pulsatile manner. In this review, we discuss two rhythms of in vivo prolactin release in female rats and the ongoing research that we and others have performed aiming to understand the mechanisms underlying them. The peptide hormone oxytocin appears to play an important role in both...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2826.2010.02012.x

    authors: Bertram R,Helena CV,Gonzalez-Iglesias AE,Tabak J,Freeman ME

    更新日期:2010-07-01 00:00:00

  • Are hormonal responses to exercise in young men with Down's syndrome related to reduced endurance performance?

    abstract::The aim of the present study was to analyse whether hormonal responses could explain an exercise limitation in Down's syndrome (DS). Fourteen young men with DS (mean age 22.5 +/- 0.7 years) and 15 controls (CONT, mean age 22.5 +/- 0.3 years) participated in the study. During a treadmill submaximal incremental test, bl...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2008.01695.x

    authors: Bricout VA,Guinot M,Faure P,Flore P,Eberhard Y,Garnier P,Juvin AF

    更新日期:2008-05-01 00:00:00

  • Expression of vesicular glutamate transporter-2.1 in medaka terminal nerve gonadotrophin-releasing hormone neurones.

    abstract::There are three paralogous genes for gonadotrophin-releasing hormone (GnRH) peptides of vertebrates in general. GnRH1, the protein product of gnrh1 gene, is the hypophysiotrophic neuropeptide, and is a critical regulator of gonadotrophin secretion, whereas GnRH2 and GnRH3 are regarded to have neuromodulatory functions...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2011.02142.x

    authors: Akazome Y,Kanda S,Oka Y

    更新日期:2011-07-01 00:00:00

  • Diverse roles of G-protein coupled receptors in the regulation of neurohypophyseal hormone secretion.

    abstract::The magnocellular neurones in the supraoptic nucleus project to the neural lobe and release vasopressin and oxytocin into the peripheral circulation, where they act on the kidney to promote fluid retention or stimulate smooth muscles in the vasculature, uterus and mammary glands to support blood pressure, promote part...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2826.2011.02268.x

    authors: Sladek CD,Song Z

    更新日期:2012-04-01 00:00:00

  • Glucose availability modulates the timing of the luteinizing hormone surge in the ewe.

    abstract::To determine if glucose availability modulates the timing of the positive feedback action of oestrogen on gonadotropin secretion, we monitored the estradiol-induced luteinizing hormone (LH) surge in sheep (n = 5/group) made transiently hypoglycemic by insulin. Experiment 1 determined an effective insulin treatment, on...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2826.1998.00264.x

    authors: Medina CL,Nagatani S,Darling TA,Bucholtz DC,Tsukamura H,Maeda K,Foster DL

    更新日期:1998-10-01 00:00:00

  • Central and peripheral immunoreactivity of melanin-concentrating hormone in hypothalamic obese and lactating rats.

    abstract::Melanin-concentrating hormone (MCH) is believed to be an important orexigenic peptide mainly localized in the lateral hypothalamic area. Its involvement in the hyperphagia induced by hypothalamic lesions and lactation remains unclear. In this study, we investigated MCH immunoreactivity in the hypothalamus using immuno...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2004.01124.x

    authors: Sun G,Tian Z,Murata T,Narita K,Honda K,Higuchi T

    更新日期:2004-01-01 00:00:00

  • Insulin-like growth factor I in the anterior pituitary of the clawed frog Xenopus laevis: immunocytochemical and autoradiographic indication for a paracrine action and corelease with prolactin.

    abstract::Insulin-like growth factor I (IGF-I) and its receptor are present in human and rat anterior pituitary. However, few data exist on the potential presence of IGF-I or its receptor in the non-mammalian pituitary and the cellular sites of IGF-I production have not been identified in any species. Thus, we investigated the ...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2826.2000.00464.x

    authors: David I,Bosshard R,Kloas W,Reinecke M

    更新日期:2000-05-01 00:00:00

  • Emotion and mood adaptations in the peripartum female:complementary contributions of GABA and oxytocin.

    abstract::Peripartum hormones and sensory cues from young modify the maternal brain in ways that can render females either at risk for, or resilient to, elevated anxiety and depression. The neurochemical systems underlying these aspects of maternal emotional and mood states include the inhibitory neurotransmitter GABA and the n...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章,评审

    doi:10.1111/jne.12188

    authors: Lonstein JS,Maguire J,Meinlschmidt G,Neumann ID

    更新日期:2014-10-01 00:00:00

  • Gonadotrophin-releasing hormone (GnRH) release in marmosets II: pulsatile release of GnRH and pituitary gonadotrophin in adult females.

    abstract::Unlike other mammals, including rodents, Old World primates and humans, common marmosets and probably all other New World primates synthesise and release chorionic gonadotrophin (CG), and not luteinising hormone (LH) from pituitary gonadotrophs. However, little is known about the physiological dynamics of gonadotrophi...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2007.01535.x

    authors: Tannenbaum PL,Schultz-Darken NJ,Woller MJ,Abbott DH

    更新日期:2007-05-01 00:00:00

  • Region-specific changes in transient receptor potential vanilloid channel expression in the vasopressin magnocellular system in hepatic cirrhosis-induced hyponatraemia.

    abstract::The present study aimed to measure the expression of transient receptor potential (TRP) channels in the magnocellular neurones of the paraventricular (PVN) and supraoptic nucleus (SON) in an animal model of hepatic cirrhosis associated with inappropriate vasopressin (AVP) release. In these studies, we used chronic bil...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2011.02273.x

    authors: Nedungadi TP,Carreño FR,Walch JD,Bathina CS,Cunningham JT

    更新日期:2012-04-01 00:00:00

  • Region-specific expression and hormonal regulation of the first exon variants of rat prolactin receptor mRNA in rat brain and anterior pituitary gland.

    abstract::Recent studies have revealed the occurrence of five first exon variants of the rat prolactin receptor mRNA, suggesting that multiple promoters direct prolactin receptor transcription in response to different regulatory factors. In the present study, regional expression of these first exon variants, as well as two prol...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2007.01565.x

    authors: Nogami H,Hoshino R,Ogasawara K,Miyamoto S,Hisano S

    更新日期:2007-08-01 00:00:00

  • Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells.

    abstract::Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF-...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.1994.tb00601.x

    authors: Bernardini S,Cianfarani S,Spagnoli A,Annicchiarico-Petruzzelli M,Melino G,Massoud R,Boscherini B,Finazzi-Agró A,Rosenfeld RG,Federici G

    更新日期:1994-08-01 00:00:00

  • Neonatal programming by neuroimmune challenge: effects on responses and tolerance to septic doses of lipopolysaccharide in adult male and female rats.

    abstract::A mild immune challenge experienced during the neonatal period leads to attenuated febrile responses to a similar challenge experienced later in life. However, the immune response to an endotoxin differs depending upon the severity of the challenge and it is not clear whether a neonatal immune challenge will also affe...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2010.01967.x

    authors: Spencer SJ,Field E,Pittman QJ

    更新日期:2010-04-01 00:00:00

  • Nurturing nature: social experiences and the brain.

    abstract::Summary How does 'nurture' change the brain? Recent evidence suggests that maternal care may shape the infant brain by turning genes 'on' or 'off' during development. Some of the genes affected are important for maternal and social behaviour leading to long-term changes in the nurturing behaviour of offspring. These s...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2009.01901.x

    authors: Champagne FA

    更新日期:2009-10-01 00:00:00

  • Pituitary network connectivity as a mechanism for the luteinising hormone surge.

    abstract::Ovulation in vertebrates is caused by a surge of luteinising hormone (LH) from the pituitary. The LH surge is initiated by rising oestradiol concentration, although the precise mechanism of oestradiol action in humans and primates is not yet understood. Recent advances in labelling and three-dimensional imaging have r...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2010.02084.x

    authors: Lyles D,Tien JH,McCobb DP,Zeeman ML

    更新日期:2010-12-01 00:00:00

  • The median eminence detects and responds to circulating prolactin in the male mouse.

    abstract::In addition to its established lactational roles, prolactin acts on multiple target tissues and its circulating levels are responsive to a range of physiological stimuli. The present study used immunohistochemistry to demonstrate that systemic administration of prolactin activates target cells in the arcuate nucleus a...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/jne.12733

    authors: Kirk SE,Grattan DR,Bunn SJ

    更新日期:2019-06-01 00:00:00

  • In vivo Intracellular Recording of Neurons in the Supraoptic Nucleus of the Rat Hypothalamus.

    abstract::Abstract Intracellular recordings were made from cells in the hypothalamic supraoptic nucleus in the urethane-anaesthetized male rat using the ventral surgical approach. Impalements lasted from 5 min to 1 h and recorded cells had an input resistance of 55 to 170 megohms. Spikes of over 50 mV were recorded from 14 cell...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.1991.tb00291.x

    authors: Dyball RE,Tasker JG,Wuarin JP,Dudek FE

    更新日期:1991-08-01 00:00:00

  • Immunological localization and ontogenetic development of inhibin alpha subunit in rat brain.

    abstract::This study examined the immunolocalization and ontogeny of the inhibin-specific alpha subunit in the brain of male rats. Immunohistochemistry using antiserum directed against the mature region of porcine inhibin alpha (1-19, Tyr20) revealed positive reactions in process-bearing cells resembling astroglia in several re...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2826.1999.00310.x

    authors: Fujimura H,Ohsawa K,Funaba M,Murata T,Murata E,Takahashi M,Abe M,Torii K

    更新日期:1999-03-01 00:00:00

  • A role for nonapeptides and dopamine in nest-building behaviour.

    abstract::During nest building in zebra finches (Taeniopygia guttata), several regions in the social behaviour network and the dopaminergic reward system, which are two neural circuits involved in social behaviour, appear to be active in male and female nest-building finches. Because the nonapeptides, mesotocin and vasotocin an...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/jne.12250

    authors: Hall ZJ,Healy SD,Meddle SL

    更新日期:2015-02-01 00:00:00

  • Long-term antidepressant treatment reduces behavioural deficits in transgenic mice with impaired glucocorticoid receptor function.

    abstract::Impaired cognitive function and enhanced activity of the hypothalamic-pituitary-adrenocortical system are among the cardinal symptoms of major depression in humans that resolve after successful antidepressant treatment. We used a transgenic mouse model expressing antisense RNA complementary to that of glucocorticoid r...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.1995.tb00724.x

    authors: Montkowski A,Barden N,Wotjak C,Stec I,Ganster J,Meaney M,Engelmann M,Reul JM,Landgraf R,Holsboer F

    更新日期:1995-11-01 00:00:00

  • Intraneuronal convergence of tactile and hormonal stimuli associated with female reproduction in rats.

    abstract::Stimulation of the vagina and cervix, by mating or manual probing, elicits many behavioral and endocrine changes associated with female reproduction in rats. We and others have identified neurons in the medial preoptic area, medial division of the bed nucleus of the stria terminalis, posterodorsal portion of the media...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.1994.tb00574.x

    authors: Tetel MJ,Celentano DC,Blaustein JD

    更新日期:1994-04-01 00:00:00

  • Activation patterns of vasopressinergic and oxytocinergic brain regions following social play exposure in juvenile male and female rats.

    abstract::Social play is a highly rewarding and motivated behavior predominately displayed by juveniles and expressed by nearly all mammalian species. Prior work suggested that the vasopressin (AVP) and oxytocin (OT) systems can regulate the expression of social play in sex-specific ways. Here we investigated whether there are ...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/jne.12582

    authors: Reppucci CJ,Gergely CK,Veenema AH

    更新日期:2018-02-09 00:00:00

  • Region- and sex-specific modulation of anxiety behaviours in the rat.

    abstract::Recent studies in both animals and humans indicate that gonadal hormones have profound control over emotional states, and certainly contribute to the increased occurrence of psychiatric illness in women. Reports, as reviewed here, suggest that two important regions of the limbic system, the central nucleus of the amyg...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2826.2007.01552.x

    authors: Toufexis D

    更新日期:2007-06-01 00:00:00

  • The role of oxytocin release in the mediobasal hypothalamus of the sheep in relation to female sexual receptivity.

    abstract::In vivo microdialysis and retrodialysis were used to investigate the role of oxytocin (OXY) release in the mediobasal hypothalamus (MBH) of the ewe in the control of sexual receptivity. Initial experiments showed that OXY release was significantly increased in ovariectomized animals treated with progesterone and oestr...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.1993.tb00359.x

    authors: Kendrick KM,Fabre-Nys C,Blache D,Goode JA,Broad KD

    更新日期:1993-02-01 00:00:00

  • NDP-MSH reduces oxidative damage induced by palmitic acid in primary astrocytes.

    abstract::Recent findings relate obesity to inflammation in key hypothalamic areas for body weight control. Hypothalamic inflammation has also been related to oxidative stress. Palmitic acid (PA) is the most abundant free fatty acid found in food, and in vitro studies indicate that it triggers a pro-inflammatory response in the...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/jne.12673

    authors: Ramírez D,Saba J,Turati J,Carniglia L,Imsen M,Mohn C,Scimonelli T,Durand D,Caruso C,Lasaga M

    更新日期:2019-02-01 00:00:00

  • A genomic analysis of subclinical hypothyroidism in hippocampus and neocortex of the developing rat brain.

    abstract::Hypothyroidism during pregnancy and the early postnatal period has severe neurological consequences for the developing offspring. The impact of milder degrees of perturbation of the thyroid axis as encompassed in conditions of subclinical hypothyroidism and hypothyroxinemia, however, has not been established. The pres...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.2008.01793.x

    authors: Royland JE,Parker JS,Gilbert ME

    更新日期:2008-12-01 00:00:00

  • Vasopressin antisense oligonucleotide induces temporary diabetes insipidus in rats.

    abstract::The purpose of this study was to downregulate the transcriptional message of arginine vasopressin (AVP) by antisense treatment. A complete phosphorothioate antisense oligodesoxynucleotide corresponding to the beginning of the coding region of rat AVP mRNA was constructed and injected into the lateral ventricle of rats...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2826.1994.tb00561.x

    authors: Skutella T,Probst JC,Engelmann M,Wotjak CT,Landgraf R,Jirikowski GF

    更新日期:1994-04-01 00:00:00

  • Immunocytochemical detection of progesterone receptor in the female rabbit forebrain: distribution and regulation by oestradiol and progesterone.

    abstract::There is no information on the neuroanatomical distribution of the progesterone receptor (PR) in the rabbit. Therefore, we mapped the distribution of PR-immunoreactive cells in the forebrain of ovariectomized female rabbits. Vehicle-injected ovariectomized rabbits showed PR-immunoreactive cells only in the infundibula...

    journal_title:Journal of neuroendocrinology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2826.2003.01070.x

    authors: Caba M,Rovirosa MJ,Beyer C,González-Mariscal G

    更新日期:2003-09-01 00:00:00