Abstract:
:The human leukocyte antigen (HLA) genes are cell-surface proteins, essential for immune cell interaction. HLA-G is known for their high immunosuppressive effect and its potential as predictive marker in breast cancer. However, nothing is known about the HLA-J and its immunosuppressive, prognostic and predictive features, as it is assumed to be a "pseudogene" by in silico sequence interpretation. HLA-J, ESR1, ERBB2, KRT5 and KRT20 mRNA expression were analysed in 29 fresh frozen breast cancer biopsies and their corresponding resectates obtained from patients treated with neoadjuvant chemotherapy (NACT). mRNA was analysed with gene specific TaqMan-based Primer/Probe sets and normalized to Calmodulin 2. All breast cancer samples did express HLA-J and frequently increased HLA-J mRNA levels after NACT. HLA-J mRNA was significantly associated with overexpression of the ESR1 mRNA status (Spearman ρ 0,5679; p = 0.0090) and KRT5 mRNA (Spearman ρ 0,6121; p = 0.0041) in breast cancer core biopsies and dominated in luminal B subtype. Kaplan Meier analysis revealed that an increase of HLA-J mRNA expression after NACT had worse progression free survival (p = 0,0096), indicating a counterreaction of tumor tissues presumably to prevent elimination by enhanced immune infiltration induced by NACT. This counterreaction is associated with worse prognosis. To our knowledge this is the first study identifying HLA-J as a new predictive marker in breast cancer being involved in immune evasion mechanisms. :Humane Leukozyten-Antigene (HLA) sind Proteine auf der Zelloberfläche, die essenziell für die Immunzellinteraktion sind. HLA-G ist für seine hohe immunosuppressive Wirkung sowie als potenzieller prädikativer Marker für Brustkrebs bekannt. Dagegen ist kaum etwas über HLA-J und seine immunosuppressiven, prognostischen und prädiktiven Eigenschaften bekannt, da es basierend auf In-silico-Sequenzanalysen als „Pseudogen“ interpretiert wurde. Die Expression von HLA-J, ESR1, ERBB2, KRT5 und KRT20 mRNA wurde in 29 frisch gefrorenen Brustkrebsbiopsien analysiert und mit den klinisch-pathologischen Daten von Patientinnen, welche mit neoadjuvanter Chemotherapie behandelt wurden, verglichen. Die mRNA-Expression wurde mit genspezifischen TaqMan-basierten Primer/Probe-Sets analysiert und auf Calmodulin 2 normalisiert. Alle Gewebeproben von Patientinnen mit Brustkrebs exprimierten HLA-J, und der HLA-J-mRNA-Spiegel war nach NACT oft erhöht. In den Brustkrebsstanzbiopsien war die HLA-J-mRNA-Expression signifikant mit der Überexpression von ESR1-mRNA (Spearmans ρ 0,5679; p = 0,0090) und KRT5-mRNA (Spearmans ρ 0,6121; p = 0,0041) assoziiert und dominierte im Luminal-B-Subtyp. Die Kaplan-Meier-Analyse zeigte, dass ein Anstieg der HLA-J-mRNA-Expression nach NACT mit einem schlechteren progressionsfreien Überleben einhergeht (p = 0,0096), womöglich als Gegenreaktion des Tumorgewebes, um eine Eliminierung durch tumorinfiltrierende Lymphozyten, welche durch eine NACT induziert wurden, zu verhindern. Diese Gegenreaktion ist mit einer schlechteren Prognose assoziiert. Soweit uns bekannt, handelt es sich hierbei um die erste Studie, die HLA-J als neuen prädiktiven Marker im Brustkrebs identifiziert hat und möglicherweise zur Immunevasion beiträgt.
journal_name
Geburtshilfe Frauenheilkdjournal_title
Geburtshilfe und Frauenheilkundeauthors
Würfel FM,Wirtz RM,Winterhalter C,Taffurelli M,Santini D,Mandrioli A,Veltrup E,Rübner M,Fasching PA,Würfel W,Zamagni Cdoi
10.1055/a-1128-6664subject
Has Abstractpub_date
2020-11-01 00:00:00pages
1123-1133issue
11eissn
0016-5751issn
1438-8804pii
11286664journal_volume
80pub_type
杂志文章abstract::Blood vessels are visible in an average of 80% of patients on whom colposcopy is performed. This percentage share increases if there are pathological changes. The following vascular types occur as characteristic criteria in cervical intraepithelial neoplasia (CIN) and in carcinoma, their classification being based on ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1036721
更新日期:1983-10-01 00:00:00
abstract::Because of the high sensitivity and specificity of the method, radio-immunologic determination of the Beta Subunit HCG in the serum is superior to other methods of monitoring the therapeutic results of the treatment of chorionic carcinoma. A ratio of 225 to 1 of HCG concentrations in the serum versus the cerebral spin...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
abstract::In healthy female volunteers the percutaneous absorption of progesterone from an ointment is studied comparing the absorption in the breast skin and that of other regions (thigh, abdomen). Before and certain times after the application progesterone serum levels are measured. A significant increase of mean serum levels...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1035875
更新日期:1987-08-01 00:00:00
abstract::In the following up of the diagnosis of the female sterility we practised 123 hysterosalpingographies (HSG) from 1972--1978. Presupposition was the proof of the ovulation by base temperature. The HSG is the beginning of the diagnosis to recognize the intracanalicular changes of the uterus and to avoid senseless treatm...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1037290
更新日期:1981-07-01 00:00:00
abstract::Following differentiated endocrinologic-gynecologic diagnosis auricular acupuncture was used in 15 women with oligoamenorrhea and 12 women with luteal body insufficiency who had come for hormone consultation because of sterility. In both groups the subsequent incidence of pregnancy was comparable to that achieved by d...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1035717
更新日期:1988-03-01 00:00:00
abstract::Without Rh-IgG prophylaxis the incidence of rhesus sensitisation increases in D-negative women with the number of subsequent pregnancies. However, our case report of a d-negative multipara demonstrates that foetal erythroblastosis does not necessarily occur in a pregnancy with initially high anti-D titres. ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1035824
更新日期:1987-04-01 00:00:00
abstract::Seven mentally retarded women were sterilized in the Department of Obstetrics and Gynaecology of Medical School Hannover between 1.6.74 and 1.6.81. The decision to operate proceeded only after careful consideration by the director of the clinic. Each case was documented with proof that improvement of the mental situat...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1036918
更新日期:1982-07-01 00:00:00
abstract::Several authors have reported on reliable postcoital contraception, using an estrogen/gestagen combination. These studies in more than a hundred women showed, that the unprotected intercourse in less than 50% took place around ovulation. Pregnancy rates from 0.7 to 2.6% were observed. From October 1987 to July 88, 50 ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1036294
更新日期:1989-12-01 00:00:00
abstract::Heterologous insemination is being established in increasing numbers in Anglo-Saxon, middle and north European countries, although some medical, legal and theological-ethical aspects are still not settled. Our experiences with this type of sterilisation in regard to marriage stability and psycho-physical development o...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::In a prospective, randomised study we compared the clinical properties of the established Redon drain with a new type of drain called "slit drain". Both types of drains were examined regarding the amount of drained fluid, the time elapsing until removal of the drain, the frequency of occlusion of the lumen as well as ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1055/s-2007-1026165
更新日期:1991-05-01 00:00:00
abstract::Breast Care Centers that were accredited according to the German Cancer Society criteria were offered to participate in a standardized patient survey in 2010, which was conducted by the Institute for Medical Sociology, Health Services Research and Rehabilitation Science, Faculty of Human Science and Faculty of Medicin...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0031-1280475
更新日期:2012-02-01 00:00:00
abstract::The influence of electric acupuncture on the sphincter of the femal urethra was checked on 15 patients with stressincontinence without gross anatomic variation recognized by means of simultaneous cystourethrometry. After 30 minutes of electric stimulation through acupuncture-needles, which were placed into the skin of...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 临床试验,杂志文章
doi:
更新日期:1975-09-01 00:00:00
abstract::A retrospective study was carried out to compare the results of in vitro fertilisation (IVF) in 20936 patients with different sperm parameters who underwent IVF in Germany between 1990 and 1993. The study was designed to evaluate prognostic factors for IVF outcome, such as sperm parameters and pre-treatment diagnosis....
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2007-1022280
更新日期:1996-03-01 00:00:00
abstract::As a part of the Central European Study a long-term study of the pubertal development of 687 Hungarian girls was undertaken and the findings compared with those of a cross-sectional study of a group of girls from Münster. In addition to assessment of the breast and pubic hair according to Tanner's classification, the ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1026624
更新日期:1988-11-01 00:00:00
abstract::The HPL-concentrations of 103 plasma samples from normal and 58 samples from risk pregnancies were analyzed with two radioimmunological assay kits from Pharmacia/Freiburg (I) and Ames/Frankfurt (II). Intra- and inter-assay-variation of both methods are similar. The absolute values of HPL-concentrations analyzed by the...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:
更新日期:1978-05-01 00:00:00
abstract::Postoperative changes in various acute-phase-proteins (alpha 1-antitrypsin, c-reactive protein), alpha 2-macroglobulin, haptoglobin, transferrin, c3-c4-complement and myoglobin as well as four endocrine parameters (TBG, SHBG, prolactin and cortisol) were studied in forty healthy women divided into two groups. Each gro...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1026628
更新日期:1988-11-01 00:00:00
abstract::Prognosis of carcinoma of the breast depends on a number of factors. Besides tumour staging, these parameters include the number of axillary lymph nodes with metastatic growths, the status of the hormonal receptors, and the histological type of the tumour, especially the tumour grading and the rate of growth correlate...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1036588
更新日期:1983-06-01 00:00:00
abstract::At the Department of Gynaecology of the RWTH-Aachen, 110 patients with palpatory and/or mammographic suspect findings were sonographically examined. By using a high resolution 10 MHz-probe with a freely mobile transducer, a new method of differential diagnostic criteria was established, pertaining to the edge of the t...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2007-1023631
更新日期:1993-01-01 00:00:00
abstract::Three cases of a vanishing twin are reported. We observed twin pregnancies in three women, who had their ultrasound examination before the 10th week of pregnancy. Positive heartbeat was registered in both embryos. When a further sonographic examination took place at the 11th week, intrauterine death of one of the embr...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1026406
更新日期:1990-12-01 00:00:00
abstract::Within two years 341 amenorrheic patients treated in our endocrinological outpatient department were listed up retrospectively in WHO-classification groups. In 30 patients a primary amenorrhea and in 311 patients a secondary amenorrhea could be registered. In 28.3% a hypothalamic pituitary disorder must be diagnosed, ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1036049
更新日期:1987-11-01 00:00:00
abstract::Marriage according to religious denomination is no longer important because of the philosophical changes in recent times. Reproductive behaviour is no longer dependant on religion for the same reasons. Faith in the naturalness and ordainment by God of the roles of the sexes is no longer prevalent. From 1960--1976 the ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::Introduction Known characteristics of patients with PCOS include infertility, menstrual disorders, hirsutism and also often insulin resistance. These symptoms increase with increasing body weight. In the LIPCOS study (Lifestyle Intervention for Patients with Polycystic Ovary Syndrome [PCOS]) long-term changes of the P...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0042-119199
更新日期:2017-01-01 00:00:00
abstract::Therapeutic results in advanced cervical cancer have not been showing any progress for more than 30 years. Only by means of systemic therapy does it seem possible, that further treatment results may be obtained similar to those in squamous cell carcinoma of the head and neck. Therefore, between 1985 and 1990 we tried ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2007-1026177
更新日期:1991-06-01 00:00:00
abstract::Recently a number of authors have published their results of conservative treatment in tubal gestations. Numerous operative techniques have been described. This review tries to summarize the different operative approaches under special consideration of microsurgical techniques in regard to the authors experience. As a...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1036118
更新日期:1985-10-01 00:00:00
abstract:INTRODUCTION:Data from the World Health Organization (WHO) demonstrates an increasing prevalence of obesity in Western countries. This study investigates the influence of obesity on the mode of delivery and the occurrence of hypoglycemia in newborns. MATERIALS AND METHODS:A retrospective analysis of all deliveries at ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-0043-108763
更新日期:2017-05-01 00:00:00
abstract::We report on a 24 + 2 weeks pregnant woman with Marfan's syndrome, who acutely developed a dissecting aortic aneurysm with aortic valve insufficiency. Emergency surgery was performed by using hypothermic extracorporeal circulation, whilst the aortic valve and ascending aorta were replaced by a synthetic graft. Foetal ...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2007-1023182
更新日期:1992-09-01 00:00:00
abstract::We report a case of non-ruptured tubal pregnancy at 38 weeks of pregnancy with the survival of mother and baby. Following salpingotomy a 1860 gr and 43 cm normal healthy male infant was delivered. The fallopian tube with placental tissue was removed by salpingectomy. Fetal development post partum and recovery of the m...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1035995
更新日期:1988-05-01 00:00:00
abstract::The article reports on a clinically unusual case of a patient with carcinoma of the breast. The "initial sign" of the disease was relapsed hypermenorrhoic permanent bleeding with severe bleeding anaemia caused by a metastatic growth in the uterus. Surgery or cytostatic treatment did not seem possible at the outset, be...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2008-1036468
更新日期:1985-05-01 00:00:00
abstract::The results of the course of birth in 71 intrauterine cases of foetal death are presented. There were spontaneous deliveries in 82% of the cases and in 4% a vacuum extraction was performed. The relatively high rate (14%) of Caesarean sections was in most cases, due to maternal indication. The introduction of prostagla...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2007-1022962
更新日期:1992-02-01 00:00:00
abstract::The presence of vulvar condylomata acuminata shows evidence HPV infection of the vulva. 49 patients with such lesions came to our hospital for laser treatment. At that time, none of the patients were aware of their cervical disease. Colposcopy and biopsy of the cervix revealed in eight (16%) of the 49 patients CIN I a...
journal_title:Geburtshilfe und Frauenheilkunde
pub_type: 杂志文章
doi:10.1055/s-2007-1023791
更新日期:1992-08-01 00:00:00