Abstract:
BACKGROUND:Cryo-electron microscopy (Cryo-EM) is widely used in the determination of the three-dimensional (3D) structures of macromolecules. Particle picking from 2D micrographs remains a challenging early step in the Cryo-EM pipeline due to the diversity of particle shapes and the extremely low signal-to-noise ratio of micrographs. Because of these issues, significant human intervention is often required to generate a high-quality set of particles for input to the downstream structure determination steps. RESULTS:Here we propose a fully automated approach (DeepCryoPicker) for single particle picking based on deep learning. It first uses automated unsupervised learning to generate particle training datasets. Then it trains a deep neural network to classify particles automatically. Results indicate that the DeepCryoPicker compares favorably with semi-automated methods such as DeepEM, DeepPicker, and RELION, with the significant advantage of not requiring human intervention. CONCLUSIONS:Our framework combing supervised deep learning classification with automated un-supervised clustering for generating training data provides an effective approach to pick particles in cryo-EM images automatically and accurately.
journal_name
BMC Bioinformaticsjournal_title
BMC bioinformaticsauthors
Al-Azzawi A,Ouadou A,Max H,Duan Y,Tanner JJ,Cheng Jdoi
10.1186/s12859-020-03809-7subject
Has Abstractpub_date
2020-11-09 00:00:00pages
509issue
1issn
1471-2105pii
10.1186/s12859-020-03809-7journal_volume
21pub_type
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