MRCQuant- an accurate LC-MS relative isotopic quantification algorithm on TOF instruments.

Abstract:

BACKGROUND:Relative isotope abundance quantification, which can be used for peptide identification and differential peptide quantification, plays an important role in liquid chromatography-mass spectrometry (LC-MS)-based proteomics. However, several major issues exist in the relative isotopic quantification of peptides on time-of-flight (TOF) instruments: LC peak boundary detection, thermal noise suppression, interference removal and mass drift correction. We propose to use the Maximum Ratio Combining (MRC) method to extract MS signal templates for interference detection/removal and LC peak boundary detection. In our method, MRCQuant, MS templates are extracted directly from experimental values, and the mass drift in each LC-MS run is automatically captured and compensated. We compared the quantification accuracy of MRCQuant to that of another representative LC-MS quantification algorithm (msInspect) using datasets downloaded from a public data repository. RESULTS:MRCQuant showed significant improvement in the number of accurately quantified peptides. CONCLUSIONS:MRCQuant effectively addresses major issues in the relative quantification of LC-MS-based proteomics data, and it provides improved performance in the quantification of low abundance peptides.

journal_name

BMC Bioinformatics

journal_title

BMC bioinformatics

authors

Haskins WE,Petritis K,Zhang J

doi

10.1186/1471-2105-12-74

subject

Has Abstract

pub_date

2011-03-15 00:00:00

pages

74

issn

1471-2105

pii

1471-2105-12-74

journal_volume

12

pub_type

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