Abstract：
:Melanism in insects is important for their physical protection, immunoreactions, and sclerotization. The vetch aphid, Megoura viciae (Buckton), has relatively strong tanning in its prothorax, head, antennae, cornicles, and legs. It was hypothesized that M. viciae may sequester the high level of l-DOPA in its host Vicia faba to help in its melanization process for ecological adaptation. To confirm this hypothesis, the amount of l-DOPA in M. viciae was modified and quantified. We first generated a Trifolium repens (clover, low l-DOPA containing) host to cut off the extra l-DOPA intake by M. viciae. The rate-limiting tyrosine hydroxylase gene of M. viciae (MV-TH) was then cloned and analyzed. To further reduce the l-DOPA level in the insect, RNAi was used to downregulate the transcriptional level of MV-TH. Our results confirmed that M. viciae could indeed sequester l-DOPA in its body, and its ample storage of this amino acid could be the reason for the strong tanning of its body. M. viciae reared on T. repens could upregulate its MV-TH to enhance l-DOPA biosynthesis and thus maintain a high level of l-DOPA. The MV-TH repression by RNAi lasted for about 3 days, successfully decreasing the l-DOPA level. Aside from a slight decrease in exuvia tanning, no other obvious change in body appearance was detected in the RNAi-treated insect. Although M. viciae can obtain most of its l-DOPA directly from its original host, its internal l-DOPA synthetase is still functional, especially when extra l-DOPA is removed from the diet. This capability to enhance its shield ensures the ecological adaptation of this insect.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Wang XX,Feng ZJ,Chen ZS,Zhang ZF,Zhang Y,Liu TXdoi
10.1016/j.jinsphys.2019.03.007subject
Has Abstractpub_date
2019-04-01 00:00:00pages
136-144eissn
0022-1910issn
1879-1611pii
S0022-1910(18)30450-5journal_volume
114pub_type
杂志文章abstract：:Odorant binding proteins (OBPs) contribute to the remarkable sensitivity of the insect's olfactory system and play an important role in insect chemical communication. In this study, we identified 11 putative cDNAs encoding OBPs (namely SexiOBP1-11) from the antennal full length cDNA library of the beet armyworm Spodop...
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：2003-08-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章,评审
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更新日期：2011-04-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：1997-10-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：1997-03-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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