Ostkpr1 functions in anther cuticle development and pollen wall formation in rice.

Abstract:

BACKGROUND:During pollen wall formation in flowering plants, a conserved metabolon consisting of acyl-CoA synthetase (ACOS), polyketide synthase (PKS) and tetraketide α-pyrone reductase (TKPR), is required for sporopollenin synthesis. Despite this, the precise function of each of these components in different species remains unclear. RESULTS:In this study, we characterized the function of OsTKPR1, a rice orthologue of Arabidopsis TKPR1. Loss of function of OsTKPR1 delayed tapetum degradation, reduced the levels of anther cuticular lipids, and impaired Ubisch body and pollen exine formation, resulting in complete male sterility. In addition, the phenylpropanoid pathway in mutant anthers was remarkably altered. Localization studies suggest that OsTKPR1 accumulates in the endoplasmic reticulum, while specific accumulation of OsTKPR1 mRNA in the anther tapetum and microspores is consistent with its function in anther and pollen wall development. CONCLUSIONS:Our results show that OsTKPR1 is indispensable for anther cuticle development and pollen wall formation in rice, providing new insights into the biochemical mechanisms of the conserved sporopollenin metabolon in flowering plants.

journal_name

BMC Plant Biol

journal_title

BMC plant biology

authors

Xu D,Qu S,Tucker MR,Zhang D,Liang W,Shi J

doi

10.1186/s12870-019-1711-4

subject

Has Abstract

pub_date

2019-03-18 00:00:00

pages

104

issue

1

issn

1471-2229

pii

10.1186/s12870-019-1711-4

journal_volume

19

pub_type

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