Abstract：
:RNA interference (RNAi) is a powerful tool for artificially manipulating gene expression in diverse organisms. In the honey bee, Apis mellifera, both long double stranded RNA (dsRNA) and small interference RNA (siRNA) have been successfully used to reduce targeted gene expression and induce specific phenotypes. However, whether dsRNA and siRNA have different effects and efficiencies in gene silencing has never been investigated in honey bees. Thus, we tested the effect of dsRNA and siRNA on the tyramine receptor 1 (tyr1), which encodes a receptor of neurotransmitter tyramine, in honey bee brains at mRNA and protein levels over time. We found that both dsRNA and siRNA achieved successful gene knockdown. The siRNA mixes affected tyr1 gene expression faster than dsRNA, and the duration of the knockdown between dsRNA and siRNA varied. We also found that the turnover rate of TYR1 protein was relatively fast, which is consistent with its role as a neurotransmitter receptor. Our study reveals the different efficiencies of dsRNA and siRNA in honey bee brains. We show that consideration of the gene regions targeted by RNAi, prior screening for RNAi molecules and combing siRNAs are important strategies to enhance RNAi efficiency.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Guo X,Wang Y,Sinakevitch I,Lei H,Smith BHdoi
10.1016/j.jinsphys.2018.10.005subject
Has Abstractpub_date
2018-01-01 00:00:00pages
47-52eissn
0022-1910issn
1879-1611pii
S0022-1910(18)30186-0journal_volume
111pub_type
杂志文章abstract：:We investigated the response of Helicoverpa armigera larvae towards ingestion of Cicer arietinum Kunitz proteinase inhibitor (CaKPI), which caused antagonistic effects on developing H. armigera larvae. CaKPI-degrading proteinases were not detectable in either control or sensitized larvae. There were negligible increas...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2005.07.005
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abstract：:The in vitro secretion of ecdysteroids from the prothoracic glands of larvae of Gryllus bimaculatus was analysed by HPLC-RIA. The primary product was identified as 3-dehydroecdysone (65-93%), with lesser amounts of ecdysone (7-35%). Production and release of ecdysteroids from the prothoracic glands are calcium-depende...
journal_title：Journal of insect physiology
pub_type： 杂志文章
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doi：10.1016/s0022-1910(02)00157-9
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2011.01.010
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abstract：:Balanced nutrition is fundamental to health and immunity. For herbivorous insects, nutrient-compositional shifts in host plants due to elevated atmospheric CO2 concentrations and temperature may compromise this balance. Therefore, understanding their immune responses to such shifts is vital if we are to predict the ou...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2015.12.002
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journal_title：Journal of insect physiology
pub_type： 杂志文章,评审
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：2009-12-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章,评审
doi：10.1016/j.jinsphys.2016.06.001
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.09.001
更新日期：2017-10-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(03)00153-7
更新日期：2003-12-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.01.003
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(01)00072-5
更新日期：2001-09-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(98)00019-5
更新日期：1998-05-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.07.008
更新日期：2017-08-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(01)00165-2
更新日期：2002-02-01 00:00:00
abstract：:Wings and legs of the gregarious desert locust, Schistocerca gregaria have been shown to be release sites of phenylacetonitrile (PAN), the major adult male-produced pheromone. However, there is limited information on the distribution of PAN within the locust. Here we show, using gas chromatography-mass spectrometry (G...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2012.03.012
更新日期：2012-08-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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journal_title：Journal of insect physiology
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2017.12.006
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journal_title：Journal of insect physiology
pub_type： 杂志文章,评审
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更新日期：2019-04-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2007.11.004
更新日期：2008-02-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2015.09.002
更新日期：2015-11-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.07.005
更新日期：2010-12-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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更新日期：2020-10-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.jinsphys.2004.01.002
更新日期：2004-05-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/s0022-1910(99)00224-3
更新日期：2000-07-01 00:00:00
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journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2010.11.009
更新日期：2011-01-01 00:00:00
abstract：:Haemocytes play crucial roles in immune responses and survival in insects. Specific cell markers have proven effective in clarifying the function and haematopoiesis of haemocytes. The silkworm Bombyx mori is a good model for studying insect haemocytes; however, little is known about haemocyte-specific markers or their...
journal_title：Journal of insect physiology
pub_type： 杂志文章
doi：10.1016/j.jinsphys.2014.10.007
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