Metabolic capability and in situ activity of microorganisms in an oil reservoir.

Abstract:

BACKGROUND:Microorganisms have long been associated with oxic and anoxic degradation of hydrocarbons in oil reservoirs and oil production facilities. While we can readily determine the abundance of microorganisms in the reservoir and study their activity in the laboratory, it has been challenging to resolve what microbes are actively participating in crude oil degradation in situ and to gain insight into what metabolic pathways they deploy. RESULTS:Here, we describe the metabolic potential and in situ activity of microbial communities obtained from the Jiangsu Oil Reservoir (China) by an integrated metagenomics and metatranscriptomics approach. Almost complete genome sequences obtained by differential binning highlight the distinct capability of different community members to degrade hydrocarbons under oxic or anoxic condition. Transcriptomic data delineate active members of the community and give insights that Acinetobacter species completely oxidize alkanes into carbon dioxide with the involvement of oxygen, and Archaeoglobus species mainly ferment alkanes to generate acetate which could be consumed by Methanosaeta species. Furthermore, nutritional requirements based on amino acid and vitamin auxotrophies suggest a complex network of interactions and dependencies among active community members that go beyond classical syntrophic exchanges; this network defines community composition and microbial ecology in oil reservoirs undergoing secondary recovery. CONCLUSION:Our data expand current knowledge of the metabolic potential and role in hydrocarbon metabolism of individual members of thermophilic microbial communities from an oil reservoir. The study also reveals potential metabolic exchanges based on vitamin and amino acid auxotrophies indicating the presence of complex network of interactions between microbial taxa within the community.

journal_name

Microbiome

journal_title

Microbiome

authors

Liu YF,Galzerani DD,Mbadinga SM,Zaramela LS,Gu JD,Mu BZ,Zengler K

doi

10.1186/s40168-017-0392-1

subject

Has Abstract

pub_date

2018-01-05 00:00:00

pages

5

issue

1

issn

2049-2618

pii

10.1186/s40168-017-0392-1

journal_volume

6

pub_type

杂志文章
  • Picodroplet partitioned whole genome amplification of low biomass samples preserves genomic diversity for metagenomic analysis.

    abstract:BACKGROUND:Whole genome amplification (WGA) is a challenging, key step in metagenomic studies of samples containing minute amounts of DNA, such as samples from low biomass environments. It is well known that multiple displacement amplification (MDA), the most commonly used WGA method for microbial samples, skews the ge...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-016-0197-7

    authors: Hammond M,Homa F,Andersson-Svahn H,Ettema TJ,Joensson HN

    更新日期:2016-10-06 00:00:00

  • Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators.

    abstract:BACKGROUND:Periodontitis is an infectious and inflammatory disease of polymicrobial etiology that can lead to the destruction of bones and tissues that support the teeth. The management of chronic periodontitis (CP) relies heavily on elimination or at least control of known pathogenic consortia associated with the dise...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/2049-2618-2-32

    authors: Galimanas V,Hall MW,Singh N,Lynch MD,Goldberg M,Tenenbaum H,Cvitkovitch DG,Neufeld JD,Senadheera DB

    更新日期:2014-08-26 00:00:00

  • Environmental toxicants in breast milk of Norwegian mothers and gut bacteria composition and metabolites in their infants at 1 month.

    abstract:BACKGROUND:Early disruption of the microbial community may influence life-long health. Environmental toxicants can contaminate breast milk and the developing infant gut microbiome is directly exposed. We investigated whether environmental toxicants in breastmilk affect the composition and function of the infant gut mic...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-019-0645-2

    authors: Iszatt N,Janssen S,Lenters V,Dahl C,Stigum H,Knight R,Mandal S,Peddada S,González A,Midtvedt T,Eggesbø M

    更新日期:2019-02-27 00:00:00

  • Gut-dependent microbial translocation induces inflammation and cardiovascular events after ST-elevation myocardial infarction.

    abstract:BACKGROUND:Post-infarction cardiovascular remodeling and heart failure are the leading cause of myocardial infarction (MI)-driven death during the past decades. Experimental observations have involved intestinal microbiota in the susceptibility to MI in mice; however, in humans, identifying whether translocation of gut...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-018-0441-4

    authors: Zhou X,Li J,Guo J,Geng B,Ji W,Zhao Q,Li J,Liu X,Liu J,Guo Z,Cai W,Ma Y,Ren D,Miao J,Chen S,Zhang Z,Chen J,Zhong J,Liu W,Zou M,Li Y,Cai J

    更新日期:2018-04-03 00:00:00

  • Genomic characterization of the uncultured Bacteroidales family S24-7 inhabiting the guts of homeothermic animals.

    abstract:BACKGROUND:Our view of host-associated microbiota remains incomplete due to the presence of as yet uncultured constituents. The Bacteroidales family S24-7 is a prominent example of one of these groups. Marker gene surveys indicate that members of this family are highly localized to the gastrointestinal tracts of homeot...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-016-0181-2

    authors: Ormerod KL,Wood DL,Lachner N,Gellatly SL,Daly JN,Parsons JD,Dal'Molin CG,Palfreyman RW,Nielsen LK,Cooper MA,Morrison M,Hansbro PM,Hugenholtz P

    更新日期:2016-07-07 00:00:00

  • Humans as holobionts: implications for prevention and therapy.

    abstract::The human gut microbiota is increasingly recognized for its important or even decisive role in health. As it becomes clear that microbiota and host mutually affect and depend on each other in an intimate relationship, a holistic view of the gut microbiota-host association imposes itself. Ideally, a stable state of equ...

    journal_title:Microbiome

    pub_type: 杂志文章,评审

    doi:10.1186/s40168-018-0466-8

    authors: van de Guchte M,Blottière HM,Doré J

    更新日期:2018-05-01 00:00:00

  • Relative and contextual contribution of different sources to the composition and abundance of indoor air bacteria in residences.

    abstract:BACKGROUND:The study of the microbial communities in the built environment is of critical importance as humans spend the majority of their time indoors. While the microorganisms in living spaces, especially those in the air, can impact health and well-being, little is known of their identity and the processes that dete...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-015-0128-z

    authors: Miletto M,Lindow SE

    更新日期:2015-12-10 00:00:00

  • Microbial differences between dental plaque and historic dental calculus are related to oral biofilm maturation stage.

    abstract:BACKGROUND:Dental calculus, calcified oral plaque biofilm, contains microbial and host biomolecules that can be used to study historic microbiome communities and host responses. Dental calculus does not typically accumulate as much today as historically, and clinical oral microbiome research studies focus primarily on ...

    journal_title:Microbiome

    pub_type: 历史文章,杂志文章

    doi:10.1186/s40168-019-0717-3

    authors: Velsko IM,Fellows Yates JA,Aron F,Hagan RW,Frantz LAF,Loe L,Martinez JBR,Chaves E,Gosden C,Larson G,Warinner C

    更新日期:2019-07-06 00:00:00

  • Tracking microbial colonization in fecal microbiota transplantation experiments via genome-resolved metagenomics.

    abstract:BACKGROUND:Fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridium difficile infection and shows promise for treating other medical conditions associated with intestinal dysbioses. However, we lack a sufficient understanding of which microbial populations successfully colonize the rec...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-017-0270-x

    authors: Lee STM,Kahn SA,Delmont TO,Shaiber A,Esen ÖC,Hubert NA,Morrison HG,Antonopoulos DA,Rubin DT,Eren AM

    更新日期:2017-05-04 00:00:00

  • Seasonal, spatial, and maternal effects on gut microbiome in wild red squirrels.

    abstract:BACKGROUND:Our understanding of gut microbiota has been limited primarily to findings from human and laboratory animals, but what shapes the gut microbiota in nature remains largely unknown. To fill this gap, we conducted a comprehensive study of gut microbiota of a well-studied North American red squirrel (Tamiasciuru...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-017-0382-3

    authors: Ren T,Boutin S,Humphries MM,Dantzer B,Gorrell JC,Coltman DW,McAdam AG,Wu M

    更新日期:2017-12-21 00:00:00

  • MetaEuk-sensitive, high-throughput gene discovery, and annotation for large-scale eukaryotic metagenomics.

    abstract:BACKGROUND:Metagenomics is revolutionizing the study of microorganisms and their involvement in biological, biomedical, and geochemical processes, allowing us to investigate by direct sequencing a tremendous diversity of organisms without the need for prior cultivation. Unicellular eukaryotes play essential roles in mo...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-020-00808-x

    authors: Levy Karin E,Mirdita M,Söding J

    更新日期:2020-04-03 00:00:00

  • Specific gut microbiome members are associated with distinct immune markers in pediatric allogeneic hematopoietic stem cell transplantation.

    abstract:BACKGROUND:Increasing evidence reveals the importance of the microbiome in health and disease and inseparable host-microbial dependencies. Host-microbe interactions are highly relevant in patients receiving allogeneic hematopoietic stem cell transplantation (HSCT), i.e., a replacement of the cellular components of the ...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-019-0745-z

    authors: Ingham AC,Kielsen K,Cilieborg MS,Lund O,Holmes S,Aarestrup FM,Müller KG,Pamp SJ

    更新日期:2019-09-13 00:00:00

  • Feasibility of using alternative swabs and storage solutions for paired SARS-CoV-2 detection and microbiome analysis in the hospital environment.

    abstract:BACKGROUND:Determining the role of fomites in the transmission of SARS-CoV-2 is essential in the hospital setting and will likely be important outside of medical facilities as governments around the world make plans to ease COVID-19 public health restrictions and attempt to safely reopen economies. Expanding COVID-19 t...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-020-00960-4

    authors: Minich JJ,Ali F,Marotz C,Belda-Ferre P,Chiang L,Shaffer JP,Carpenter CS,McDonald D,Gilbert J,Allard SM,Allen EE,Knight R,Sweeney DA,Swafford AD

    更新日期:2021-01-22 00:00:00

  • Wild bees and their nests host Paenibacillus bacteria with functional potential of avail.

    abstract:BACKGROUND:In previous studies, the gram-positive firmicute genus Paenibacillus was found with significant abundances in nests of wild solitary bees. Paenibacillus larvae is well-known for beekeepers as a severe pathogen causing the fatal honey bee disease American foulbrood, and other members of the genus are either s...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-018-0614-1

    authors: Keller A,Brandel A,Becker MC,Balles R,Abdelmohsen UR,Ankenbrand MJ,Sickel W

    更新日期:2018-12-22 00:00:00

  • Comparative meta-RNA-seq of the vaginal microbiota and differential expression by Lactobacillus iners in health and dysbiosis.

    abstract:BACKGROUND:Bacterial vaginosis (BV), the most common vaginal condition of reproductive-aged women, is associated with a highly diverse and heterogeneous microbiota. Here we present a proof-of-principle analysis to uncover the function of the microbiota using meta-RNA-seq to uncover genes and pathways that potentially d...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/2049-2618-1-12

    authors: Macklaim JM,Fernandes AD,Di Bella JM,Hammond JA,Reid G,Gloor GB

    更新日期:2013-04-12 00:00:00

  • Mobile antibiotic resistome in wastewater treatment plants revealed by Nanopore metagenomic sequencing.

    abstract:BACKGROUND:Wastewater treatment plants (WWTPs) are recognized as hotspots for horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs). Despite our understanding of the composition and distribution of ARGs in WWTPs, the genetic location, host, and fate of ARGs remain largely unknown. RESULTS:In this study,...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-019-0663-0

    authors: Che Y,Xia Y,Liu L,Li AD,Yang Y,Zhang T

    更新日期:2019-03-21 00:00:00

  • Correction to: Microbiome definition re-visited: old concepts and new challenges.

    abstract::An amendment to this paper has been published and can be accessed via the original article. ...

    journal_title:Microbiome

    pub_type: 已发布勘误

    doi:10.1186/s40168-020-00905-x

    authors: Berg G,Rybakova D,Fischer D,Cernava T,Vergès MC,Charles T,Chen X,Cocolin L,Eversole K,Corral GH,Kazou M,Kinkel L,Lange L,Lima N,Loy A,Macklin JA,Maguin E,Mauchline T,McClure R,Mitter B,Ryan M,Sarand I,Smidt H

    更新日期:2020-08-20 00:00:00

  • Gut microbiota-derived propionate mediates the neuroprotective effect of osteocalcin in a mouse model of Parkinson's disease.

    abstract:BACKGROUND:Parkinson's disease (PD) is a neurodegenerative disorder with no absolute cure. The evidence of the involvement of gut microbiota in PD pathogenesis suggests the need to identify certain molecule(s) derived from the gut microbiota, which has the potential to manage PD. Osteocalcin (OCN), an osteoblast-secret...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-020-00988-6

    authors: Hou YF,Shan C,Zhuang SY,Zhuang QQ,Ghosh A,Zhu KC,Kong XK,Wang SM,Gong YL,Yang YY,Tao B,Sun LH,Zhao HY,Guo XZ,Wang WQ,Ning G,Gu YY,Li ST,Liu JM

    更新日期:2021-01-31 00:00:00

  • Impact of investigational microbiota therapeutic RBX2660 on the gut microbiome and resistome revealed by a placebo-controlled clinical trial.

    abstract:BACKGROUND:Intestinal microbiota restoration can be achieved by complementing a subject's perturbed microbiota with that of a healthy donor. Recurrent Clostridioides difficile infection (rCDI) is one key application of such treatment. Another emerging application of interest is reducing antibiotic-resistant genes (ARGs...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-020-00907-9

    authors: Kwak S,Choi J,Hink T,Reske KA,Blount K,Jones C,Bost MH,Sun X,Burnham CD,Dubberke ER,Dantas G,CDC Prevention Epicenter Program.

    更新日期:2020-08-31 00:00:00

  • Facultative methanotrophs are abundant at terrestrial natural gas seeps.

    abstract:BACKGROUND:Natural gas contains methane and the gaseous alkanes ethane, propane and butane, which collectively influence atmospheric chemistry and cause global warming. Methane-oxidising bacteria, methanotrophs, are crucial in mitigating emissions of methane as they oxidise most of the methane produced in soils and the...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-018-0500-x

    authors: Farhan Ul Haque M,Crombie AT,Ensminger SA,Baciu C,Murrell JC

    更新日期:2018-06-28 00:00:00

  • A comparison of methods used to unveil the genetic and metabolic pool in the built environment.

    abstract:BACKGROUND:A majority of indoor residential microbes originate from humans, pets, and outdoor air and are not adapted to the built environment (BE). Consequently, a large portion of the microbes identified by DNA-based methods are either dead or metabolically inactive. Although many exceptions have been noted, the ribo...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-018-0453-0

    authors: Gomez-Silvan C,Leung MHY,Grue KA,Kaur R,Tong X,Lee PKH,Andersen GL

    更新日期:2018-04-16 00:00:00

  • Differential dynamics of microbial community networks help identify microorganisms interacting with residue-borne pathogens: the case of Zymoseptoria tritici in wheat.

    abstract:BACKGROUND:Wheat residues are a crucial determinant of the epidemiology of Septoria tritici blotch, as they support the sexual reproduction of the causal agent Zymoseptoria tritici. We aimed to characterize the effect of infection with this fungal pathogen on the microbial communities present on wheat residues and to i...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-019-0736-0

    authors: Kerdraon L,Barret M,Laval V,Suffert F

    更新日期:2019-08-30 00:00:00

  • Clustering co-abundant genes identifies components of the gut microbiome that are reproducibly associated with colorectal cancer and inflammatory bowel disease.

    abstract:BACKGROUND:Whole-genome "shotgun" (WGS) metagenomic sequencing is an increasingly widely used tool for analyzing the metagenomic content of microbiome samples. While WGS data contains gene-level information, it can be challenging to analyze the millions of microbial genes which are typically found in microbiome experim...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-019-0722-6

    authors: Minot SS,Willis AD

    更新日期:2019-08-01 00:00:00

  • Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens.

    abstract:BACKGROUND:There is a paucity of data regarding the microbial constituents of tobacco products and their impacts on public health. Moreover, there has been no comparative characterization performed on the bacterial microbiota associated with the addition of menthol, an additive that has been used by tobacco manufacture...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-017-0235-0

    authors: Chopyk J,Chattopadhyay S,Kulkarni P,Claye E,Babik KR,Reid MC,Smyth EM,Hittle LE,Paulson JN,Cruz-Cano R,Pop M,Buehler SS,Clark PI,Sapkota AR,Mongodin EF

    更新日期:2017-02-15 00:00:00

  • Systematic assessment of secondary bile acid metabolism in gut microbes reveals distinct metabolic capabilities in inflammatory bowel disease.

    abstract:BACKGROUND:The human gut microbiome performs important functions in human health and disease. A classic example for host-gut microbial co-metabolism is host biosynthesis of primary bile acids and their subsequent deconjugation and transformation by the gut microbiome. To understand these system-level host-microbe inter...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-019-0689-3

    authors: Heinken A,Ravcheev DA,Baldini F,Heirendt L,Fleming RMT,Thiele I

    更新日期:2019-05-15 00:00:00

  • The gut mycobiome of the Human Microbiome Project healthy cohort.

    abstract:BACKGROUND:Most studies describing the human gut microbiome in healthy and diseased states have emphasized the bacterial component, but the fungal microbiome (i.e., the mycobiome) is beginning to gain recognition as a fundamental part of our microbiome. To date, human gut mycobiome studies have primarily been disease c...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-017-0373-4

    authors: Nash AK,Auchtung TA,Wong MC,Smith DP,Gesell JR,Ross MC,Stewart CJ,Metcalf GA,Muzny DM,Gibbs RA,Ajami NJ,Petrosino JF

    更新日期:2017-11-25 00:00:00

  • An integrated meta-omics approach reveals substrates involved in synergistic interactions in a bisphenol A (BPA)-degrading microbial community.

    abstract:BACKGROUND:Understanding microbial interactions in engineering bioprocesses is important to enhance and optimize performance outcomes and requires dissection of the multi-layer complexities of microbial communities. However, unraveling microbial interactions as well as substrates involved in complex microbial communiti...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-019-0634-5

    authors: Yu K,Yi S,Li B,Guo F,Peng X,Wang Z,Wu Y,Alvarez-Cohen L,Zhang T

    更新日期:2019-02-06 00:00:00

  • Fast and simple protein-alignment-guided assembly of orthologous gene families from microbiome sequencing reads.

    abstract:BACKGROUND:Microbiome sequencing projects typically collect tens of millions of short reads per sample. Depending on the goals of the project, the short reads can either be subjected to direct sequence analysis or be assembled into longer contigs. The assembly of whole genomes from metagenomic sequencing reads is a ver...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-017-0233-2

    authors: Huson DH,Tappu R,Bazinet AL,Xie C,Cummings MP,Nieselt K,Williams R

    更新日期:2017-01-25 00:00:00

  • Fine metagenomic profile of the Mediterranean stratified and mixed water columns revealed by assembly and recruitment.

    abstract:BACKGROUND:The photic zone of aquatic habitats is subjected to strong physicochemical gradients. To analyze the fine-scale variations in the marine microbiome, we collected seven samples from a single offshore location in the Mediterranean at 15 m depth intervals during a period of strong stratification, as well as two...

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-018-0513-5

    authors: Haro-Moreno JM,López-Pérez M,de la Torre JR,Picazo A,Camacho A,Rodriguez-Valera F

    更新日期:2018-07-10 00:00:00

  • Bacteriophage-prokaryote dynamics and interaction within anaerobic digestion processes across time and space.

    abstract:BACKGROUND:Bacteriophage-prokaryote dynamics and interaction are believed to be important in governing microbiome composition and ecosystem functions, yet our limited knowledge of the spatial and temporal variation in phage and prokaryotic community compositions precludes accurate assessment of their roles and impacts....

    journal_title:Microbiome

    pub_type: 杂志文章

    doi:10.1186/s40168-017-0272-8

    authors: Zhang J,Gao Q,Zhang Q,Wang T,Yue H,Wu L,Shi J,Qin Z,Zhou J,Zuo J,Yang Y

    更新日期:2017-05-31 00:00:00