Abstract:
BACKGROUND:In previous studies, the gram-positive firmicute genus Paenibacillus was found with significant abundances in nests of wild solitary bees. Paenibacillus larvae is well-known for beekeepers as a severe pathogen causing the fatal honey bee disease American foulbrood, and other members of the genus are either secondary invaders of European foulbrood or considered a threat to honey bees. We thus investigated whether Paenibacillus is a common bacterium associated with various wild bees and hence poses a latent threat to honey bees visiting the same flowers. RESULTS:We collected 202 samples from 82 individuals or nests of 13 bee species at the same location and screened each for Paenibacillus using high-throughput sequencing-based 16S metabarcoding. We then isolated the identified strain Paenibacillus MBD-MB06 from a solitary bee nest and sequenced its genome. We did find conserved toxin genes and such encoding for chitin-binding proteins, yet none specifically related to foulbrood virulence or chitinases. Phylogenomic analysis revealed a closer relationship to strains of root-associated Paenibacillus rather than strains causing foulbrood or other accompanying diseases. We found anti-microbial evidence within the genome, confirmed by experimental bioassays with strong growth inhibition of selected fungi as well as gram-positive and gram-negative bacteria. CONCLUSIONS:The isolated wild bee associate Paenibacillus MBD-MB06 is a common, but irregularly occurring part of wild bee microbiomes, present on adult body surfaces and guts and within nests especially in megachilids. It was phylogenetically and functionally distinct from harmful members causing honey bee colony diseases, although it shared few conserved proteins putatively toxic to insects that might indicate ancestral predisposition for the evolution of insect pathogens within the group. By contrast, our strain showed anti-microbial capabilities and the genome further indicates abilities for chitin-binding and biofilm-forming, suggesting it is likely a useful associate to avoid fungal penetration of the bee cuticula and a beneficial inhabitant of nests to repress fungal threats in humid and nutrient-rich environments of wild bee nests.
journal_name
Microbiomejournal_title
Microbiomeauthors
Keller A,Brandel A,Becker MC,Balles R,Abdelmohsen UR,Ankenbrand MJ,Sickel Wdoi
10.1186/s40168-018-0614-1subject
Has Abstractpub_date
2018-12-22 00:00:00pages
229issue
1issn
2049-2618pii
10.1186/s40168-018-0614-1journal_volume
6pub_type
杂志文章相关文献
Microbiome文献大全abstract:BACKGROUND:Wheat residues are a crucial determinant of the epidemiology of Septoria tritici blotch, as they support the sexual reproduction of the causal agent Zymoseptoria tritici. We aimed to characterize the effect of infection with this fungal pathogen on the microbial communities present on wheat residues and to i...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-019-0736-0
更新日期:2019-08-30 00:00:00
abstract::Following publication of the original article [1], the authors reported an error in Figure 1 and a text on page 13. In Figure 1, some areas are missing and the correct figure is presented here. On page 13, the sentence should be updated to the following (change has been indicated in bold italics). ...
journal_title:Microbiome
pub_type: 已发布勘误
doi:10.1186/s40168-019-0763-x
更新日期:2019-11-14 00:00:00
abstract:BACKGROUND:Natural gas contains methane and the gaseous alkanes ethane, propane and butane, which collectively influence atmospheric chemistry and cause global warming. Methane-oxidising bacteria, methanotrophs, are crucial in mitigating emissions of methane as they oxidise most of the methane produced in soils and the...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-018-0500-x
更新日期:2018-06-28 00:00:00
abstract:BACKGROUND:Gastrointestinal mucosal injury (mucositis), commonly affecting the oral cavity, is a clinically significant yet incompletely understood complication of cancer chemotherapy. Although antineoplastic cytotoxicity constitutes the primary injury trigger, the interaction of oral microbial commensals with mucosal ...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-019-0679-5
更新日期:2019-04-25 00:00:00
abstract:BACKGROUND:Aphids are known to live in symbiosis with specific bacteria, called endosymbionts which can be classified as obligate or accessory. Buchnera aphidicola is generally the only obligatory symbiont present in aphids, supplying essential nutrients that are missing in the plants phloem to its host. Pentalonia nig...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-015-0130-5
更新日期:2015-12-15 00:00:00
abstract:BACKGROUND:Determining the role of fomites in the transmission of SARS-CoV-2 is essential in the hospital setting and will likely be important outside of medical facilities as governments around the world make plans to ease COVID-19 public health restrictions and attempt to safely reopen economies. Expanding COVID-19 t...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-020-00960-4
更新日期:2021-01-22 00:00:00
abstract::Recent studies have conflicting data regarding the presence of intra-amniotic microbiota. Viral communities are increasingly recognized as important although overlooked components of the human microbiota. It is unknown if the developing fetus is exposed to a community of viruses (virome). Given the debate over the exi...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-018-0475-7
更新日期:2018-05-11 00:00:00
abstract:BACKGROUND:Enteric fermentation by farmed ruminant animals is a major source of methane and constitutes the second largest anthropogenic contributor to global warming. Reducing methane emissions from ruminants is needed to ensure sustainable animal production in the future. Methane yield varies naturally in sheep and i...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-016-0201-2
更新日期:2016-10-19 00:00:00
abstract:BACKGROUND:Plants have developed defense strategies for phytopathogen and herbivore protection via coordinated metabolic mechanisms. Low-molecular weight metabolites produced within plant tissues, such as salicylic acid, represent one such mechanism which likely mediates plant - microbe interactions above and below gro...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-019-0668-8
更新日期:2019-05-18 00:00:00
abstract::Human microbiome reference datasets provide epidemiological context for researchers, enabling them to uncover new insights into their own data through meta-analyses. In addition, large and comprehensive reference sets offer a means to develop or test hypotheses and can pave the way for addressing practical study desig...
journal_title:Microbiome
pub_type: 杂志文章,评审
doi:10.1186/s40168-015-0117-2
更新日期:2015-11-04 00:00:00
abstract:BACKGROUND:Wastewater treatment plants (WWTPs) are recognized as hotspots for horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs). Despite our understanding of the composition and distribution of ARGs in WWTPs, the genetic location, host, and fate of ARGs remain largely unknown. RESULTS:In this study,...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-019-0663-0
更新日期:2019-03-21 00:00:00
abstract::The human gut microbiota is increasingly recognized for its important or even decisive role in health. As it becomes clear that microbiota and host mutually affect and depend on each other in an intimate relationship, a holistic view of the gut microbiota-host association imposes itself. Ideally, a stable state of equ...
journal_title:Microbiome
pub_type: 杂志文章,评审
doi:10.1186/s40168-018-0466-8
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:Whole-genome "shotgun" (WGS) metagenomic sequencing is an increasingly widely used tool for analyzing the metagenomic content of microbiome samples. While WGS data contains gene-level information, it can be challenging to analyze the millions of microbial genes which are typically found in microbiome experim...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-019-0722-6
更新日期:2019-08-01 00:00:00
abstract::The vaginal microbiota plays a significant role in health and disease of the female reproductive tract. Next-generation sequencing techniques based upon the analysis of bacterial 16S rRNA genes permit in-depth study of vaginal microbial community structure to a level of detail not possible with standard culture-based ...
journal_title:Microbiome
pub_type: 杂志文章,评审
doi:10.1186/s40168-016-0203-0
更新日期:2016-11-01 00:00:00
abstract:BACKGROUND:Metallo-β-lactamases are bacterial enzymes that provide resistance to carbapenems, the most potent class of antibiotics. These enzymes are commonly encoded on mobile genetic elements, which, together with their broad substrate spectrum and lack of clinically useful inhibitors, make them a particularly proble...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-017-0353-8
更新日期:2017-10-12 00:00:00
abstract:BACKGROUND:Lake sediments harbor diverse microbial communities that cycle carbon and nutrients while being constantly colonized and potentially buried by organic matter sinking from the water column. The interaction of activity and burial remained largely unexplored in aquatic sediments. We aimed to relate taxonomic co...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-017-0255-9
更新日期:2017-04-08 00:00:00
abstract:BACKGROUND:Archaea are one of the least-studied members of the gut-dwelling autochthonous microbiota. Few studies have reported the dominance of methanogens in the archaeal microbiome (archaeome) of the human gut, although limited information regarding the diversity and abundance of other archaeal phylotypes is availab...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-020-00894-x
更新日期:2020-08-04 00:00:00
abstract:BACKGROUND:Fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridium difficile infection and shows promise for treating other medical conditions associated with intestinal dysbioses. However, we lack a sufficient understanding of which microbial populations successfully colonize the rec...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-017-0270-x
更新日期:2017-05-04 00:00:00
abstract:BACKGROUND:A majority of indoor residential microbes originate from humans, pets, and outdoor air and are not adapted to the built environment (BE). Consequently, a large portion of the microbes identified by DNA-based methods are either dead or metabolically inactive. Although many exceptions have been noted, the ribo...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-018-0453-0
更新日期:2018-04-16 00:00:00
abstract:BACKGROUND:Our understanding of gut microbiota has been limited primarily to findings from human and laboratory animals, but what shapes the gut microbiota in nature remains largely unknown. To fill this gap, we conducted a comprehensive study of gut microbiota of a well-studied North American red squirrel (Tamiasciuru...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-017-0382-3
更新日期:2017-12-21 00:00:00
abstract:BACKGROUND:This proof-of-principle study examines whether postnatal, low-dose exposure to environmental chemicals modifies the composition of gut microbiome. Three chemicals that are widely used in personal care products-diethyl phthalate (DEP), methylparaben (MPB), triclosan (TCS)-and their mixture (MIX) were administ...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-016-0173-2
更新日期:2016-06-14 00:00:00
abstract:BACKGROUND:Bacterial predation is an important selective force in microbial community structure and dynamics. However, only a limited number of predatory bacteria have been reported, and their predatory strategies and evolutionary adaptations remain elusive. We recently isolated a novel group of bacterial predators, Br...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-020-00902-0
更新日期:2020-08-31 00:00:00
abstract:BACKGROUND:Sub-therapeutic antibiotics are widely used as growth promoters in the poultry industry; however, the resulting antibiotic resistance threatens public health. A plant-derived growth promoter, Macleaya cordata extract (MCE), with effective ingredients of benzylisoquinoline alkaloids, is a potential alternativ...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-018-0590-5
更新日期:2018-11-27 00:00:00
abstract:BACKGROUND:Deep-sea hydrothermal vents are hotspots for productivity and biodiversity. Thermal pyrolysis and circulation produce fluids rich in hydrocarbons and reduced compounds that stimulate microbial activity in surrounding sediments. Several studies have characterized the diversity of Guaymas Basin (Gulf of Califo...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-017-0322-2
更新日期:2017-08-23 00:00:00
abstract:BACKGROUND:The correct establishment of the human gut microbiota represents a crucial development that commences at birth. Different hypotheses propose that the infant gut microbiota is derived from, among other sources, the mother's fecal/vaginal microbiota and human milk. RESULTS:The composition of bifidobacterial c...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-017-0282-6
更新日期:2017-06-26 00:00:00
abstract::Human genome-wide association studies (GWASs) have recurrently estimated lower heritability estimates than familial studies. Many explanations have been suggested to explain these lower estimates, including that a substantial proportion of genetic variation and gene-by-environment interactions are unmeasured in typica...
journal_title:Microbiome
pub_type: 信件
doi:10.1186/s40168-020-00839-4
更新日期:2020-06-08 00:00:00
abstract:BACKGROUND:Bacterial vaginosis (BV), the most common vaginal condition of reproductive-aged women, is associated with a highly diverse and heterogeneous microbiota. Here we present a proof-of-principle analysis to uncover the function of the microbiota using meta-RNA-seq to uncover genes and pathways that potentially d...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/2049-2618-1-12
更新日期:2013-04-12 00:00:00
abstract:BACKGROUND:Microorganisms have long been associated with oxic and anoxic degradation of hydrocarbons in oil reservoirs and oil production facilities. While we can readily determine the abundance of microorganisms in the reservoir and study their activity in the laboratory, it has been challenging to resolve what microb...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-017-0392-1
更新日期:2018-01-05 00:00:00
abstract:BACKGROUND:The nasopharynx is a reservoir for pathogens associated with respiratory illnesses, such as asthma. Next-generation sequencing (NGS) has been used to characterize the nasopharyngeal microbiome during health and disease. Most studies so far have surveyed the nasopharynx as a whole; however, less is known abou...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-016-0170-5
更新日期:2016-06-16 00:00:00
abstract:BACKGROUND:Most studies describing the human gut microbiome in healthy and diseased states have emphasized the bacterial component, but the fungal microbiome (i.e., the mycobiome) is beginning to gain recognition as a fundamental part of our microbiome. To date, human gut mycobiome studies have primarily been disease c...
journal_title:Microbiome
pub_type: 杂志文章
doi:10.1186/s40168-017-0373-4
更新日期:2017-11-25 00:00:00