Prevalence of porA pseudogene deletion among Neisseria gonorrhoeae isolates referred to the UK's Gonococcal Resistance to Antimicrobials Surveillance Program.

Abstract:

:porA pseudogene-negative Neisseria gonorrhoeae isolates produce false-negative results when examined by polymerase chain reaction (PCR) with porA pseudogene targets. In the present study, 533 representative gonococcal isolates received in 2011 via the Gonococcal Resistance to Antimicrobials Surveillance Program were examined to determine the prevalence of porA-negative isolates. Less than 0.4% (2/533) of isolates were found to be reproducibly negative with the porA real-time PCR but were confirmed as N. gonorrhoeae with molecular, biochemical and immunological confirmatory tests. Sequencing revealed both isolates contained the Neisseria meningitidis porA gene. Low prevalence indicates that although these isolates do not present a major public health problem, microbiologists should remain vigilant.

journal_name

Sex Health

journal_title

Sexual health

authors

Toby M,Saunders P,Cole M,Grigorjev V,Alexander S,Ison C

doi

10.1071/SH16162

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

392-393

issue

4

eissn

1448-5028

issn

1449-8987

pii

SH16162

journal_volume

14

pub_type

杂志文章