Abstract:
:porA pseudogene-negative Neisseria gonorrhoeae isolates produce false-negative results when examined by polymerase chain reaction (PCR) with porA pseudogene targets. In the present study, 533 representative gonococcal isolates received in 2011 via the Gonococcal Resistance to Antimicrobials Surveillance Program were examined to determine the prevalence of porA-negative isolates. Less than 0.4% (2/533) of isolates were found to be reproducibly negative with the porA real-time PCR but were confirmed as N. gonorrhoeae with molecular, biochemical and immunological confirmatory tests. Sequencing revealed both isolates contained the Neisseria meningitidis porA gene. Low prevalence indicates that although these isolates do not present a major public health problem, microbiologists should remain vigilant.
journal_name
Sex Healthjournal_title
Sexual healthauthors
Toby M,Saunders P,Cole M,Grigorjev V,Alexander S,Ison Cdoi
10.1071/SH16162subject
Has Abstractpub_date
2017-08-01 00:00:00pages
392-393issue
4eissn
1448-5028issn
1449-8987pii
SH16162journal_volume
14pub_type
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