Abstract:
BACKGROUND:To build and evaluate intrinsic coagulation factor assays which can eliminate the effect of lupus anticoagulants (LAC). METHODS:Commercial silica clotting time confirmatory (SCT-C) reagent containing sufficient synthetic phospholipid and routine activated partial thromboplastin time (APTT) reagent were each used for one-stage detection of FVIII, FIX, and FXI activities, in samples with or without LAC, and the results were compared. RESULTS:For samples without LAC, consistent results of FVIII, FIX, and FXI using both SCT-C reagent and APTT reagent were obtained. For samples with LAC, the assays with SCT-C reagent not only could eliminate the effect of strong lupus anticoagulants but also needed fewer dilutions than that with routine APTT reagent. CONCLUSIONS:The intrinsic factor detections by SCT-C reagent are credible and convenient to be used for samples with LAC.
journal_name
Clin Labjournal_title
Clinical laboratoryauthors
Tang N,Yin Sdoi
10.7754/Clin.Lab.2015.151106subject
Has Abstractpub_date
2016-07-01 00:00:00pages
1363-1365issue
7eissn
1433-6510journal_volume
62pub_type
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