Transcriptional response of mysid crustacean, Americamysis bahia, is affected by subchronic exposure to nonylphenol.

Abstract:

:Nonylphenol (NP) has been classified as an endocrine-disrupting chemical. In this study, we conducted mysid DNA microarray analysis with which has 2240 oligo DNA probes to observe differential gene expressions in mysid crustacean (Americamysis bahia) exposed to 1, 3, 10 and 30 μg/l of NP for 14 days. As a result, we found 31, 27, 39 and 68 genes were differentially expressed in the respective concentrations. Among these genes, the expressions of five particular genes were regulated in a similar manner at all concentrations of the NP exposure. So, we focused on one gene encoding cuticle protein, and another encoding cuticular protein analogous to peritrophins 1-H precursor. These genes were down-regulated by NP exposure in a dose-dependent manner, and it suggested that they were related in a reduction of the number of molting in mysids. Thus, they might become useful molecular biomarker candidates to evaluate molting inhibition in mysids.

journal_name

Ecotoxicol Environ Saf

authors

Uchida M,Hirano M,Ishibashi H,Kobayashi J,Kagami Y,Koyanagi A,Kusano T,Koga M,Arizono K

doi

10.1016/j.ecoenv.2016.07.037

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

360-5

eissn

0147-6513

issn

1090-2414

pii

S0147-6513(16)30296-2

journal_volume

133

pub_type

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