Sampling volume in root studies: the pitfalls of under-sampling exposed using accumulation curves.

Abstract:

:Root systems are important for global models of below-ground carbon and nutrient cycling. Notoriously difficult sampling methods and the fractal distribution of root diameters in the soil make data being used in these models especially susceptible to error resulting from under-sampling. We applied the concept of species accumulation curves to root data to quantify the extent of under-sampling inherent to minirhizotron and soil coring sampling for both root uptake and carbon content studies. Based on differences in sample size alone, minirhizotron sampling missed approximately one third of the root diameters observed by soil core sampling. Sample volumes needed to encounter 90% of root diameters averaged 2481 cm(3) for uptake studies and 5878 cm(3) for root carbon content studies. These results show that small sample volumes encounter a non-representative sample of the overall root pool, and provide future guidelines for determining optimal sample volumes in root studies.

journal_name

Ecol Lett

journal_title

Ecology letters

authors

Taylor BN,Beidler KV,Cooper ER,Strand AE,Pritchard SG

doi

10.1111/ele.12119

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

862-9

issue

7

eissn

1461-023X

issn

1461-0248

journal_volume

16

pub_type

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