Abstract:
:Phosphorylated Spo0A is a master regulator of stationary phase development in the model bacterium Bacillus subtilis, controlling the formation of spores, biofilms, and cells competent for transformation. We have monitored the rate of transcription of the spo0A gene during growth in sporulation medium using promoter fusions to firefly luciferase. This rate increases sharply during transient diauxie-like pauses in growth rate and then declines as growth resumes. In contrast, the rate of transcription of an rRNA gene decreases and increases in parallel with the growth rate, as expected for stable RNA synthesis. The growth pause-dependent bursts of spo0A transcription, which reflect the activity of the spo0A vegetative promoter, are largely independent of all known regulators of spo0A transcription. Evidence is offered in support of a "passive regulation" model in which RNA polymerase stops transcribing rRNA genes during growth pauses, thus becoming available for the transcription of spo0A. We show that the bursts are followed by the production of phosphorylated Spo0A, and we propose that they represent initial responses to stress that bring the average cell closer to the thresholds for transition to bimodally expressed developmental responses. Measurement of the numbers of cells expressing a competence marker before and after the bursts supports this hypothesis. In the absence of ppGpp, the increase in spo0A transcription that accompanies the entrance to stationary phase is delayed and sporulation is markedly diminished. In spite of this, our data contradicts the hypothesis that sporulation is initiated when a ppGpp-induced depression of the GTP pool relieves repression by CodY. We suggest that, while the programmed induction of sporulation that occurs in stationary phase is apparently provoked by increased flux through the phosphorelay, bet-hedging stochastic transitions to at least competence are induced by bursts in transcription.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Mirouze N,Prepiak P,Dubnau Ddoi
10.1371/journal.pgen.1002048subject
Has Abstractpub_date
2011-04-01 00:00:00pages
e1002048issue
4eissn
1553-7390issn
1553-7404journal_volume
7pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::Nephrolithiasis is a common nephrologic disorder with complex etiology. To identify the genetic factor(s) for nephrolithiasis, we conducted a three-stage genome-wide association study (GWAS) using a total of 5,892 nephrolithiasis cases and 17,809 controls of Japanese origin. Here we found three novel loci for nephroli...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002541
更新日期:2012-01-01 00:00:00
abstract::During neural circuit formation, most axons are guided to complex environments, coming into contact with multiple potential synaptic partners. However, it is critical that they recognize specific neurons with which to form synapses. Here, we utilize the split GFP-based marker Neuroligin-1 GFP Reconstitution Across Syn...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007312
更新日期:2018-05-09 00:00:00
abstract::Insecticide resistance in malaria vectors threatens to reverse recent gains in malaria control. Deciphering patterns of gene flow and resistance evolution in malaria vectors is crucial to improving control strategies and preventing malaria resurgence. A genome-wide survey of Anopheles funestus genetic diversity Africa...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008822
更新日期:2020-06-04 00:00:00
abstract::Considerable evidence now supports the idea that the moderate telomere lengthening produced by recombinational telomere elongation (RTE) in a Kluyveromyces lactis telomerase deletion mutant occurs through a roll-and-spread mechanism. However, it is unclear whether this mechanism can account for other forms of RTE that...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003017
更新日期:2012-01-01 00:00:00
abstract::Genome-wide transcription start site (TSS) profiles of the enterobacteria Escherichia coli and Klebsiella pneumoniae were experimentally determined through modified 5' RACE followed by deep sequencing of intact primary mRNA. This identified 3,746 and 3,143 TSSs for E. coli and K. pneumoniae, respectively. Experimental...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002867
更新日期:2012-01-01 00:00:00
abstract::Double strand breaks (DSBs) and interstrand crosslinks (ICLs) are toxic DNA lesions that can be repaired through multiple pathways, some of which involve shared proteins. One of these proteins, DNA Polymerase θ (Pol θ), coordinates a mutagenic DSB repair pathway named microhomology-mediated end joining (MMEJ) and is a...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006813
更新日期:2017-05-25 00:00:00
abstract::The mammalian Y chromosome plays a critical role in spermatogenesis. However, the exact functions of each gene in the Y chromosome have not been completely elucidated, partly owing to difficulties in gene targeting analysis of the Y chromosome. Zfy was first proposed to be a sex determination factor, but its function ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006578
更新日期:2017-01-23 00:00:00
abstract::Demographic models built from genetic data play important roles in illuminating prehistorical events and serving as null models in genome scans for selection. We introduce an inference method based on the joint frequency spectrum of genetic variants within and between populations. For candidate models we numerically c...
journal_title:PLoS genetics
pub_type: 历史文章,杂志文章
doi:10.1371/journal.pgen.1000695
更新日期:2009-10-01 00:00:00
abstract::The deregulation of metabolism is a hallmark of aging. As such, changes in the expression of metabolic genes and the profiles of amino acid levels are features associated with aging animals. We previously reported that the levels of most amino acids decline with age in Caenorhabditis elegans (C. elegans). Glycine, in ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007633
更新日期:2019-03-07 00:00:00
abstract::Bacteria can arrest their own growth and proliferation upon nutrient depletion and under various stressful conditions to ensure their survival. However, the molecular mechanisms responsible for suppressing growth and arresting the cell cycle under such conditions remain incompletely understood. Here, we identify post-...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005342
更新日期:2015-07-02 00:00:00
abstract::Genome-wide association studies (GWAS) have identified over 40 loci that affect risk of coronary artery disease (CAD) and the causal mechanisms at the majority of loci are unknown. Recent studies have suggested that many causal GWAS variants influence disease through altered transcriptional regulation in disease-relev...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005061
更新日期:2015-04-02 00:00:00
abstract::The interplay of microbiota and the human host is physiologically crucial in health and diseases. The beneficial effects of lactic acid bacteria (LAB), permanently colonizing the human intestine or transiently obtained from food, have been extensively reported. However, the molecular understanding of how LAB modulate ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008145
更新日期:2019-05-23 00:00:00
abstract::Polymyxin is the last line of defense against severe infections caused by carbapenem-resistant gram-negative pathogens. The emergence of transferable MCR-1/2 polymyxin resistance greatly challenges the renewed interest in colistin (polymyxin E) for clinical treatments. Recent studies have suggested that Moraxella spec...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007389
更新日期:2018-05-14 00:00:00
abstract::In all sexually reproducing organisms, cells of the germ line must transition from mitosis to meiosis. In mice, retinoic acid (RA), the extrinsic signal for meiotic initiation, activates transcription of Stra8, which is required for meiotic DNA replication and the subsequent processes of meiotic prophase. Here we repo...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004541
更新日期:2014-08-07 00:00:00
abstract::MicroRNAs (miRNAs) post-transcriptionally regulate the expression of thousands of distinct mRNAs. While some regulatory interactions help to maintain basal cellular functions, others are likely relevant in more specific settings, such as response to stress. Here we describe such a role for the mir-290-295 cluster, the...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002054
更新日期:2011-05-01 00:00:00
abstract::Altered daily patterns of hormone action are suspected to contribute to metabolic disease. It is poorly understood how the adrenal glucocorticoid hormones contribute to the coordination of daily global patterns of transcription and metabolism. Here, we examined diurnal metabolite and transcriptome patterns in a zebraf...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006512
更新日期:2016-12-12 00:00:00
abstract::Down syndrome (DS), commonly caused by an extra copy of chromosome 21 (chr21), occurs in approximately one out of 700 live births. Precisely how an extra chr21 causes over 80 clinically defined phenotypes is not yet clear. Reduced representation bisulfite sequencing (RRBS) analysis at single base resolution revealed D...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003515
更新日期:2013-06-01 00:00:00
abstract::Genome-wide association studies (GWAS) have become increasingly common due to advances in technology and have permitted the identification of differences in single nucleotide polymorphism (SNP) alleles that are associated with diseases. However, while typical GWAS analysis techniques treat markers individually, comple...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002101
更新日期:2011-06-01 00:00:00
abstract::Recent and classical work has revealed biologically and medically significant subtypes in complex diseases and traits. However, relevant subtypes are often unknown, unmeasured, or actively debated, making automated statistical approaches to subtype definition valuable. We propose reverse GWAS (RGWAS) to identify and v...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008009
更新日期:2019-04-05 00:00:00
abstract::Rare individuals with inactivating mutations in the Huntington's disease gene (HTT) exhibit variable abnormalities that imply essential HTT roles during organ development. Here we report phenotypes produced when increasingly severe hypomorphic mutations in the murine HTT orthologue Htt, (HdhneoQ20, HdhneoQ50, HdhneoQ1...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007765
更新日期:2019-03-21 00:00:00
abstract::While there has been much recent focus on the ecological causes of adaptive diversification, we know less about the genetic nature of the trade-offs in resource use that create and maintain stable, diversified ecotypes. Here we show how a regulatory genetic change can contribute to sympatric diversification caused by ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0030015
更新日期:2007-01-19 00:00:00
abstract::For many genes, proper gene expression requires coordinated and dynamic interactions between multiple regulatory elements, each of which can either promote or silence transcription. In Drosophila, the complexity of the regulatory landscape is further complicated by the tight physical pairing of homologous chromosomes,...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008152
更新日期:2019-05-10 00:00:00
abstract::Filamentous fungi produce diverse secondary metabolites (SMs) essential to their ecology and adaptation. Although each SM is typically produced by only a handful of species, global SM production is governed by widely conserved transcriptional regulators in conjunction with other cellular processes, such as development...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005096
更新日期:2015-03-18 00:00:00
abstract::Fragile X syndrome (FXS) is a form of inherited mental retardation in humans that results from expansion of a CGG repeat in the Fmr1 gene. Recent studies suggest a role of astrocytes in neuronal development. However, the mechanisms involved in the regulation process of astrocytes from FXS remain unclear. In this study...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003172
更新日期:2012-01-01 00:00:00
abstract::Dishevelled (Dvl) proteins are important signaling components of both the canonical beta-catenin/Wnt pathway, which controls cell proliferation and patterning, and the planar cell polarity (PCP) pathway, which coordinates cell polarity within a sheet of cells and also directs convergent extension cell (CE) movements t...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000259
更新日期:2008-11-01 00:00:00
abstract::High-altitude hypoxia (reduced inspired oxygen tension due to decreased barometric pressure) exerts severe physiological stress on the human body. Two high-altitude regions where humans have lived for millennia are the Andean Altiplano and the Tibetan Plateau. Populations living in these regions exhibit unique circula...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001116
更新日期:2010-09-09 00:00:00
abstract::The major transition to eusociality required the evolution of a switch to canalize development into either a reproductive or a helper, the nature of which is currently unknown. Following predictions from the 'theory of facilitated variation', we identify sex differentiation pathways as promising candidates because of ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005952
更新日期:2016-03-31 00:00:00
abstract::Mp is an irradiation-induced mouse mutation associated with microphthalmia, micropinna and hind limb syndactyly. We show that Mp is caused by a 660 kb balanced inversion on chromosome 18 producing reciprocal 3-prime gene fusion events involving Fbn2 and Isoc1. The Isoc1-Fbn2 fusion gene (Isoc1(Mp)) mRNA has a frameshi...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003998
更新日期:2013-01-01 00:00:00
abstract::Magnetosomes of magnetotactic bacteria contain well-ordered nanocrystals for magnetic navigation and have recently emerged as the most sophisticated model system to study the formation of membrane bounded organelles in prokaryotes. Magnetosome biosynthesis is thought to begin with the formation of a dedicated compartm...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006101
更新日期:2016-06-10 00:00:00
abstract::The liver possesses a remarkable regenerative capacity based partly on the ability of hepatocytes to re-enter the cell cycle and divide to replace damaged cells. This capability is substantially reduced upon chronic damage, but it is not clear if this is a cause or consequence of liver disease. Here, we investigate wh...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1009084
更新日期:2020-11-04 00:00:00