Abstract:
:Isolation of adipose-derived stem cells (ASCs) typically involves 8+ hours of intense effort, requiring specialized equipment and reagents. Here, we present an improved technique for isolating viable populations of mesenchymal stem cells from lipoaspirate saline fractions within 30 minutes. Importantly, the cells exhibit remarkable similarities to those obtained using the traditional isolation protocols, in terms of their multipotent differentiation potential and immunophenotype. Reducing the acquisition time of ASCs is critical for advancing regenerative medicine therapeutics, and our approach provides rapid and simple techniques for enhanced isolation and expansion of patient-derived mesenchymal stem cells.
journal_name
Organogenesisjournal_title
Organogenesisauthors
Francis MP,Sachs PC,Elmore LW,Holt SEdoi
10.4161/org.6.1.10019subject
Has Abstractpub_date
2010-01-01 00:00:00pages
11-4issue
1eissn
1547-6278issn
1555-8592journal_volume
6pub_type
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