Computerized sperm motility analysis in toxicity bioassays: a new approach to pore water quality assessment.

Abstract:

:The aim of this study was to test the sensitivity of computerized sperm motility analysis in the sea urchin Paracentrotus lividus as the endpoint in toxicity bioassays. The tested matrices were pore water samples collected in an agriculture-impacted Mediterranean lagoon, Lake Varano (Italy). Two standardized bioassays were also conducted as controls, the P. lividus spermiotoxicity test and the Vibrio fischeri (Microtox®) test. VCL (curvilinear velocity), VSL (straight line velocity), VAP (average path velocity), and the percentage of rapid spermatozoa recorded by the Sperm Class Analyzer® system showed high sensitivity and discrimination ability, to a degree comparable with the larval development endpoint of the spermiotoxicity test. The test evaluated in this study requires small volumes of matrices, involves minimal sample manipulation, and can easily be extended to many other bioindicator species. It may therefore be considered a promising "quick response tool" following hazardous events that may adversely affect an aquatic ecosystem.

journal_name

Ecotoxicol Environ Saf

authors

Fabbrocini A,Di Stasio M,D'Adamo R

doi

10.1016/j.ecoenv.2010.05.003

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

1588-95

issue

7

eissn

0147-6513

issn

1090-2414

pii

S0147-6513(10)00090-4

journal_volume

73

pub_type

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