Abstract:
:Since Robert Koch and colleagues found agar to be an effective gelling agent over a century ago, the pure culture method using agar plates has long been a standard of microbiology. Agar is undoubtedly easy to handle and useful for culture of microorganisms, but recent discovery of the ubiquity of microorganisms that cannot be cultured on agar raises a question: is agar really the best agent? In this study, we investigated the effect of two gelling agents, agar and gellan gum, on colony formation of a diverse array of microorganisms (total 108 strains) newly isolated from freshwater sediments and a representative microorganism as a slow grower on agar medium, Gemmatimonas aurantiaca, to clarify (i) whether they can grow on both agar and gellan gum plates, and (ii) the difference in time required for colony formation between the two gelling agents. Interestingly, 22 of 108 isolates showed no ability to form any visible colonies on the agar medium but did so on the gellan gum medium, and showed low 16S rRNA gene sequence similarities to their closest species. The remaining 86 isolates grew on both agar and gellan gum, but 52 of them grew much faster on gellan gum than on agar. Moreover, gellan gum also significantly stimulated the colony formation of the representative slow-growing microorganism G. aurantiaca. Our results demonstrate that the gelling agent is a crucial factor for the growth of bacteria on plate media, and that alternatives to agar will be very important for increasing the culturability of yet-to-be cultured microorganisms.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Tamaki H,Hanada S,Sekiguchi Y,Tanaka Y,Kamagata Ydoi
10.1111/j.1462-2920.2009.01907.xsubject
Has Abstractpub_date
2009-07-01 00:00:00pages
1827-34issue
7eissn
1462-2912issn
1462-2920pii
EMI1907journal_volume
11pub_type
杂志文章abstract::Biofilm formation is crucial to the environmental survival and transmission of Vibrio cholerae, the facultative human pathogen responsible for the disease cholera. During its infectious cycle, V. cholerae experiences fluctuations in temperature within the aquatic environment and during the transition between human hos...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12799
更新日期:2015-11-01 00:00:00
abstract::Thermus thermophilus HB27 is well known for its extraordinary trait of high frequencies of natural transformation, which is considered a major mechanism of horizontal gene transfer. We show that the DNA translocator of T. thermophilus binds and transports DNA from members of all three domains. These results, together ...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2008.01801.x
更新日期:2009-04-01 00:00:00
abstract::Contaminated soils are subject to diurnal and seasonal temperature variations during on-site ex-situ bioremediation processes. We assessed how diurnal temperature variations similar to that in summer at the site from which petroleum hydrocarbon-contaminated soil was collected affect the soil microbial community and th...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12846
更新日期:2015-12-01 00:00:00
abstract::Methylotrophy is a metabolic capability possessed by microorganisms that allows them to build biomass and to obtain energy from organic substrates containing no carbon-carbon bonds (C1 compounds, such as methane, methanol, etc.). This phenomenon in microbial physiology has been a subject of study for over 100 years, e...
journal_title:Environmental microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1462-2920.2011.02464.x
更新日期:2011-10-01 00:00:00
abstract::Fragilariopsis is a dominating psychrophilic diatom genus in polar sea ice. The two species Fragilariopsis cylindrus and Fragilariopsis curta are able to grow and divide below freezing temperature of sea water and above average sea water salinity. Here we show that antifreeze proteins (AFPs), involved in cold adaptati...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2009.02149.x
更新日期:2010-04-01 00:00:00
abstract::As part of a study on the microbiology of chlorate reduction, several new dissimilatory chlorate-reducing bacteria were isolated from a broad diversity of environments. One of these, strain CKB, was selected for a more complete characterization. Strain CKB was enriched and isolated from paper mill waste with acetate a...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1046/j.1462-2920.1999.00042.x
更新日期:1999-08-01 00:00:00
abstract::The Black Sea chemocline represents the largest extant habitat of anoxygenic phototrophic bacteria and harbours a monospecific population of Chlorobium phylotype BS-1. High-sensitivity measurements of underwater irradiance and sulfide revealed that the optical properties of the overlying water column were similar acro...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2010.02178.x
更新日期:2010-05-01 00:00:00
abstract::In warm regions, agricultural fields are occupied by complex Aspergillus flavus communities composed of isolates in many vegetative compatibility groups (VCGs) with varying abilities to produce highly toxic, carcinogenic aflatoxins. Aflatoxin contamination is reduced with biocontrol products that enable atoxigenic iso...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.15122
更新日期:2020-08-01 00:00:00
abstract::Certain filamentous cyanobacteria, including Aphanizomenon ovalisporum, are potentially toxic owing to the formation of the hepatotoxin cylindrospermopsin. We previously identified a gene cluster in A. ovalisporum likely to be involved in cylindrospermopsin biosynthesis, including amidinotransferase (aoaA) and polyket...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2007.01519.x
更新日期:2008-04-01 00:00:00
abstract::The ascidian Cystodytes dellechiajei (Della Valle, 1877) (phylum Chordata, class Ascidiacea, family Polycitoridae) is a colonial tunicate that inhabits benthic rock environments in the Atlantic, Pacific and Indian Oceans, as well as the Mediterranean Sea. Its life cycle has two phases, the adult sessile colony and the...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2006.01170.x
更新日期:2007-02-01 00:00:00
abstract::Understanding the ecosystem functioning in the dark portion of the ocean is a challenge that microbial ecologists are still facing. Due to the large volume, the global deep Ocean plays a central role in the regulation of climate, possibly buffering the rise of atmospheric carbon dioxide if processes of CO2 fixation co...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13641
更新日期:2017-03-01 00:00:00
abstract::A method was developed to assess the genetic structure of Gaeumannomyces graminis var. tritici (Ggt) populations and test the hypothesis of an association between disease level in the field with changes in pathogen populations. A long-term wheat monoculture experiment, established since 1994, generated different take-...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2004.00637.x
更新日期:2004-11-01 00:00:00
abstract::Deciphering the complex interconnecting bacterial responses to the presence of aromatic compounds is required to gain an integrated understanding of how aromatic catabolic processes function in relation to their genome and environmental context. In addition to the properties of the catabolic enzymes themselves, regula...
journal_title:Environmental microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1462-2920.2003.00472.x
更新日期:2003-12-01 00:00:00
abstract::Sulphoquinovosyldiacylglycerols (SQDG) are polar sulphur-containing membrane lipids, whose presence has been related to a microbial strategy to adapt to phosphate deprivation. In this study, we have targeted the sqdB gene coding the uridine 5'-diphosphate-sulphoquinovose (UDP-SQ) synthase involved in the SQDG biosynth...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12202
更新日期:2014-03-01 00:00:00
abstract::The stratified water column of the Black Sea produces a vertical succession of redox zones, stimulating microbial activity at the interfaces. Our study of intact polar membrane lipids (IPLs) in suspended particulate matter and sediments highlights their potential as biomarkers for assessing the taxonomic composition o...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2009.01999.x
更新日期:2009-10-01 00:00:00
abstract::The ability of the product of a desired reaction to activate a bacterial transcriptional regulator was exploited to develop genetic traps that render the catalytic activity born by a DNA clone into a selectable/scorable phenotype. We established this strategy with a system to expose the activity of dehydrochlorinases ...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2006.00983.x
更新日期:2006-03-01 00:00:00
abstract::Halophilic Bacteriovorax (Bx), formerly known as the marine Bdellovibrio, are Gram-negative, predatory bacteria found in saltwater systems. To assess their genetic diversity and geographical occurrence, the small subunit rRNA (ssu-rRNA) gene sequences were analysed from 111 marine, salt lake and estuarine isolates rec...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2007.01362.x
更新日期:2007-10-01 00:00:00
abstract::Methyl-coenzyme M reductase (MCR) has been originally identified to catalyse the final step of the methanogenesis pathway. About 20 years ago anaerobic methane-oxidizing archaea (ANME) were discovered that use MCR enzymes to activate methane. ANME thrive at the thermodynamic limit of life, are slow-growing, and in mos...
journal_title:Environmental microbiology
pub_type: 杂志文章,评审
doi:10.1111/1462-2920.15057
更新日期:2020-05-04 00:00:00
abstract::The structure of bacterial communities in first-year spring and summer sea ice differs from that in source seawaters, suggesting selection during ice formation in autumn or taxon-specific mortality in the ice during winter. We tested these hypotheses by weekly sampling (January-March 2004) of first-year winter sea ice...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2010.02179.x
更新日期:2010-07-01 00:00:00
abstract::The wide distribution of diverse nitrogenase (nifH) genes affiliated with those of heterotrophic bacteria in marine and estuarine waters indicates ubiquity and an ecologically relevant role for heterotrophic N2 -fixers (diazotrophs) in aquatic nitrogen (N) cycling. However, the lack of cultivated representatives curre...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12335
更新日期:2014-10-01 00:00:00
abstract::Phage predation is one of the key forces that shape genetic diversity in bacterial genomes. Phages are also believed to act as modulators of the microbiota composition and, consequently, as agents that drive bacterial speciation in complex bacterial communities. Very little is known about the occurrence and genetic va...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13154
更新日期:2016-07-01 00:00:00
abstract::It has generally been accepted that the formation of granules in anaerobic wastewater treatment systems requires a hydraulic upflow pattern. To evaluate this hypothesis, we operated an anaerobic migrating blanket reactor (AMBR) without a hydraulic upflow pattern, using a synthetic wastewater containing acetate, propio...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2004.00597.x
更新日期:2004-04-01 00:00:00
abstract::The Bacillus cereus Group comprises organisms that are widely distributed in the environment and are of health and economic interest. We demonstrate an 'ecotypic' structure of populations in the B. cereus Group using (i) molecular data from Fluorescent Amplified Fragment Length Polymorphism patterns, ribosomal gene se...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2007.01495.x
更新日期:2008-04-01 00:00:00
abstract::Microorganisms that thrive in acidic environments are endowed with specialized molecular mechanisms to survive under this extremely harsh condition. In this work, we performed functional screening of six metagenomic libraries from planktonic and rhizosphere microbial communities of the Tinto River, an extremely acidic...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12021
更新日期:2013-04-01 00:00:00
abstract::The population genomics of Pseudomonas aeruginosa was analysed by genome sequencing of representative strains of the 15 most frequent clonal complexes in the P. aeruginosa population and of the five most common clones from the environment of which so far no isolate from a human infection has been detected. Gene annota...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12606
更新日期:2015-01-01 00:00:00
abstract::Model denitrifiers convert NO3- to N2 , but it appears that a significant fraction of natural populations are truncated, conducting only one or two steps of the pathway. To better understand the diversity of partial denitrifiers in soil and whether discrepancies arise between the presence of known N-oxide reductase ge...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13250
更新日期:2017-06-01 00:00:00
abstract::Global change models indicate that rainfall patterns are likely to shift towards more extreme events concurrent with increasing atmospheric carbon dioxide concentration ([CO2 ]). Both changes in [CO2 ] and rainfall regime are known to impact above- and belowground communities, but the interactive effects of these glob...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13802
更新日期:2017-08-01 00:00:00
abstract::The contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of D- and L-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35 degrees N to 5 degrees S). Fluorescence in situ hybridization (FISH) rev...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2008.01627.x
更新日期:2008-07-01 00:00:00
abstract::Enhanced biological phosphorus removal (EBPR) exploits the metabolism of polyphosphate-accumulating organisms (PAOs) to remove excess phosphorus (P) from wastewater treatment. Candidatus Accumulibacter phosphatis (Accumulibacter) is the most abundant and well-studied PAO in EBPR systems. In a previous study, we detect...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14576
更新日期:2019-04-01 00:00:00
abstract::Ecteinascidin 743 (ET-743, Yondelis) is a clinically approved chemotherapeutic natural product isolated from the Caribbean mangrove tunicate Ecteinascidia turbinata. Researchers have long suspected that a microorganism may be the true producer of the anticancer drug, but its genome has remained elusive due to our inab...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12908
更新日期:2015-10-01 00:00:00