Abstract：
BACKGROUND AND OBJECTIVES:Occult hepatitis B virus (HBV) infection might allow the release of viremic units into the blood supply network if blood is tested only for hepatitis B surface antigen (HBsAg). The aim of our study was to evaluate the actual prevalence, viral load and genotype of occult HBV infections among first-time blood donors in north-western Italy and to suggest a way to minimize risks of transmission of this infection. DESIGN AND METHODS:We assayed 6313 consecutive blood donors for antibodies to HBV core antigen (anti-HBc) in addition to mandatory screening. HBsAg-negative/anti-HBc-positive donors were assayed for antibodies to HBsAg (anti-HBs) and for HBV-DNA using COBAS Ampliscreen HBV (Roche) on individual donations. All HBV-DNA-positive samples underwent confirmatory testing with additional polymerase chain reaction-based assays. RESULTS:The prevalence of anti-HBc positive subjects was 4.85%. Fourteen out of 288 blood donors (4.86%) were confirmed to have circulating HBV-DNA at a low level (range 8-108 IU/mL). All viremic donors were also anti-HBs-positive. INTERPRETATION AND CONCLUSIONS:We estimate that in north-western Italy up to 2298 units per million donated units from first-time donors may contain HBV-DNA. The risk of an HBV-DNA positive unit from an occult carrier being released into the blood supply is more than 100 times higher than the estimated residual risk related to the window phase of HBV infection in our country. The potential infectivity of these units is debated, but their use cannot be considered safe at least in immunocompromised patients.
journal_name
Haematologicajournal_title
Haematologicaauthors
Manzini P,Girotto M,Borsotti R,Giachino O,Guaschino R,Lanteri M,Testa D,Ghiazza P,Vacchini M,Danielle F,Pizzi A,Valpreda C,Castagno F,Curti F,Magistroni P,Abate ML,Smedile A,Rizzetto Mdoi
10.3324/haematol.11224subject
Has Abstractpub_date
2007-12-01 00:00:00pages
1664-70issue
12eissn
0390-6078issn
1592-8721pii
92/12/1664journal_volume
92pub_type
杂志文章相关文献
HAEMATOLOGICA文献大全abstract：:Sixteen patients with stage III multiple myeloma (MM) and a median age of 51 years were treated with autografting followed by reduced intensity conditioning allotransplantation (RICT). Nine patients are alive in remission at a median of 30 months after their transplants, one patient is alive in relapse and 6 patients ...
journal_title：Haematologica
pub_type： 临床试验,信件
doi：
更新日期：2004-12-01 00:00:00
abstract：BACKGROUND:Acute myeloid leukemia is a clonal hematopoietic malignant disease; about 45-50% of cases do not have detectable chromosomal abnormalities. Here, we identified hidden genomic alterations and novel disease-related regions in normal karyotype acute myeloid leukemia/myelodysplastic syndrome samples. DESIGN AND...
journal_title：Haematologica
pub_type： 杂志文章
doi：10.3324/haematol.13024
更新日期：2009-02-01 00:00:00
abstract：:The present study reports a method for the easy, rapid and cost effective detection of heterozygous large deletions. As a model gene all exons of the antithrombin gene were amplified in a one tube multiplex polymerase chain reaction (PCR) and the products separated according to their size by reverse-phase ion-pair hig...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:The limited value of qualitative reverse transcription polymerase chain-reaction (RT-PCR) for monitoring chronic myeloid leukemia (CML) patients has prompted the development of quantitative assays. We have developed a quantitative real-time PCR (QC-PCR) method in the LightCycler, based on the ...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Hereditary hyperferritinemia-cataract syndrome (HHCS) is an autosomal dominant disorder characterized by bilateral cataracts and increased serum and tissue L-ferritin, in the absence of iron overload. The deregulation of ferritin production is caused by heterogeneous mutations in the iron regul...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:A case of stiff-man syndrome (SMS), a rare and dramatic CNS disease characterized by continuous muscle activity and painful spasms resembling a chronic form of tetanus, occurring in a patient with Hodgkin's disease (HD) is reported. The patient developed the clinical features of SMS at the same time as the HD relapse....
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:The detection of recombinant human erythropoietin (r-HuEPO) abuse by athletes remains problematic. The main aim of this study was to demonstrate that the five indirect markers of altered erythropoiesis identified in our earlier work were reliable evidence of current or recently discontinued r-...
journal_title：Haematologica
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：
更新日期：2001-02-01 00:00:00
abstract：:Inherited bone marrow failure syndromes are experiments of nature characterized by impaired hematopoiesis with cancer and leukemia predisposition. The mutations associated with inherited bone marrow failure syndromes affect fundamental cellular pathways, such as DNA repair, telomere maintenance, or proteostasis. How t...
journal_title：Haematologica
pub_type： 杂志文章,评审
doi：10.3324/haematol.2018.196105
更新日期：2019-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:The strongest risk factor identified for inhibitor development in people with severe hemophilia A is the type of factor VIII gene mutation. The objective of this study was to evaluate the mutation type dependent concordance rate of inhibitor formation in siblings. DESIGN AND METHODS:The gene ...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：:The bone marrow microenvironment regulates normal and malignant hematopoiesis, but the underlying molecular mechanisms remain poorly defined. Using a chimeric mice model, we demonstrate that Twist1 deletion in the bone marrow microenvironment results in alteration of multiple niche cells as well as downregulated expre...
journal_title：Haematologica
pub_type： 杂志文章
doi：10.3324/haematol.2018.190652
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:With the development and refinement of guidance modalities for percutaneous biopsies, many investigators have reported studies supporting the role of guided core needle biopsy in the diagnosis of mediastinal lymphoma. The aims of this report are to evaluate the efficacy of findings at core need...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:High levels of erythrocyte destruction in sickle cell anemia (SCA) result in chronic hyperbilirubinemia, with cholelithiasis occurring in a subset of patients. We investigated whether susceptibility to cholelithiasis in SCA was associated with the promoter polymorphism of the 5?-diphosphate-gl...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：2005-02-01 00:00:00
abstract：BACKGROUND:Vgamma9Vdelta2 T lymphocytes are regarded as promising mediators of cancer immunotherapy due to their capacity to eliminate multiple experimental tumors, particularly within those of hematopoietic origin. However, Vgamma9Vdelta2 T-cell based lymphoma clinical trials have suffered from the lack of biomarkers ...
journal_title：Haematologica
pub_type： 杂志文章
doi：10.3324/haematol.2009.020602
更新日期：2010-08-01 00:00:00
abstract：:Mucosa-associated lymphoid tissue (MALT) lymphoma originates from a background of diverse chronic inflammatory disorders at various anatomic sites. The genetics underlying its development, particularly in those associated with autoimmune disorders, is poorly characterized. By whole exome sequencing of 21 cases of MALT...
journal_title：Haematologica
pub_type： 杂志文章
doi：10.3324/haematol.2018.191601
更新日期：2018-08-01 00:00:00
abstract：BACKGROUND:Usefulness of iron chelation therapy in myelodysplastic patients is still under debate but many authors suggest its possible role in improving survival of low-risk myelodysplastic patients. Several reports have described an unexpected effect of iron chelators, such as an improvement in hemoglobin levels, in ...
journal_title：Haematologica
pub_type： 杂志文章
doi：10.3324/haematol.2009.016824
更新日期：2010-08-01 00:00:00
abstract：BACKGROUND:The functional activity of the organic cation transporter 1 (OCT-1) protein (OCT-1 activity) is an excellent predictor of molecular response and progression-free survival in patients with newly diagnosed chronic phase chronic myeloid leukemia treated with imatinib as front-line therapy. DESIGN AND METHODS:I...
journal_title：Haematologica
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.3324/haematol.2011.056457
更新日期：2012-06-01 00:00:00
abstract：:Previous studies identified the Ser/Thr protein kinase, AKT, as a therapeutic target in thrombo-inflammatory diseases. Here we report that specific inhibition of AKT with ARQ 092, an orally-available AKT inhibitor currently in phase Ib clinical trials as an anti-cancer drug, attenuates the adhesive function of neutrop...
journal_title：Haematologica
pub_type： 杂志文章
doi：10.3324/haematol.2016.151159
更新日期：2017-02-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:The role of hematopoietic chimerism after allogeneic stem cell transplantation (SCT) for acute leukemia remains controversial. We studied the relationship between hematopoietic chimerism and several prognostic variables on the outcome of SCT in patients with acute leukemia. DESIGN AND METHODS...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：:Immune dysregulation is a mechanism contributing to ineffective hematopoiesis in a subset of myelodysplastic syndrome patients. We report the first US multicenter non-randomized, phase II trial examining the efficacy of rabbit(r)-anti-thymocyte globulin using 2.5 mg/kg/day administered daily for 4 doses. The primary e...
journal_title：Haematologica
pub_type： 杂志文章,多中心研究
doi：10.3324/haematol.2012.083345
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:We analyzed the factors that affected the number and quality of peripheral blood stem cells (PBSC) collected for transplant in order to establish a minimum threshold for rapid hematopoietic recovery. DESIGN AND METHODS:From January 1995 to November 1996, a consecutive series of 67 patients, wi...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:The regulation of platelets by oxidants is critical for vascular health and may explain thrombotic complications in diseases such as diabetes and dementia, but remains poorly understood. Here, we describe a novel technique combining electron paramagnetic resonance spectroscopy and turbidimetry, which has been utilized...
journal_title：Haematologica
pub_type： 杂志文章
doi：10.3324/haematol.2018.208819
更新日期：2019-09-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Altered adhesive interaction between bone marrow (BM) stroma and progenitors in chronic myeloid leukemia (CML) may be in part caused by abnormal expression of cell adhesion molecules (CAMs) on malignant progenitor cells. Treatment of CML with interferon-a (IFN-a) re-establishes normal hemopoies...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:Granulocyte colony-stimulating factor (G-CSF) can be administered after a peripheral blood stem cell transplantation with the aim of accelerating neutrophil recovery. In a randomized, single-blind study we studied a new administration schedule of G-CSF in this context. ...
journal_title：Haematologica
pub_type： 临床试验,信件,随机对照试验
doi：
更新日期：2004-09-01 00:00:00
abstract：BACKGROUND:Cutaneous T-cell lymphomas (CTCL) represent about the 80% of skin lymphomas and comprise a heterogeneous group of diseases with respect to clinical presentation, outcome, histologic and immunophenotypic features. In the recent years, data have been accumulated indicating that clinical and biological differen...
journal_title：Haematologica
pub_type： 杂志文章,评审
doi：
更新日期：2004-11-01 00:00:00
abstract：:A new method, combining seminested polymerase chain reaction (PCR) with heteroduplex analysis, was utilized to detect follicular lymphoma (FL) cells in peripheral blood. The method, based on the detection of IgH rearrangements in DNA, detected the presence of monoclonal B cells in FL patients with a high frequency. ...
journal_title：Haematologica
pub_type： 信件
doi：
更新日期：2005-02-01 00:00:00
abstract：:The long-term repopulating capacity of mobilized peripheral blood (MPB) CD34+ cells can be abrogated during ex vivo expansion due to cell-cycle related changes. In this study, we traced cell cycle kinetic and quiescent status of CD34+ cells during ex vivo expansion with exogenous cytokines. The addition of interleukin...
journal_title：Haematologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Interleukin-10 failed to modify either the percentage of bcl-2+ cells or the number of bcl-2 molecules, or to reduce 2-chlorodeoxyadenosine- and fludarabine-induced apoptosis. The cytokine at 0.1 ng/mL induced an increase of cell survival both in the absence or in the presence of 2-chlorodeoxyadenosine, while no diffe...
journal_title：Haematologica
pub_type： 信件
doi：
更新日期：1998-11-01 00:00:00
abstract：:Renewing interest in cancer immunotherapy reflects the excellent results that have been obtained in animal models and the promising results in early clinical trails with dendritic cell (DC) based approaches. The central role that DCs play in the initiation of an immune response raises the possibility of using them to ...
journal_title：Haematologica
pub_type： 杂志文章,评审
doi：
更新日期：2000-02-01 00:00:00
abstract：:The sialic-acid-binding immunoglobulin-like lectin SIGLEC-G is a negative regulator of B-cell receptor-mediated calcium signaling. Its deficiency leads to reduced turnover and increased proliferation and survival of murine B-1a cells. Siglecg(-/-) mice show a premature expansion of polyclonal CD5(+) B cells in the spl...
journal_title：Haematologica
pub_type： 杂志文章
doi：10.3324/haematol.2013.100230
更新日期：2014-08-01 00:00:00
abstract：:We report a family with type I and type III protein S (PS) deficiency, which showed to be phenotypic variants of the same genetic disease. Direct sequencing analysis of the PROS1 gene was performed to establish the genotype. The ratio of protein C antigen and total PS antigen levels (protein C/S ratio) was used to cla...
journal_title：Haematologica
pub_type： 信件
doi：
更新日期：2006-08-01 00:00:00
