Abstract:
:The secondary structure of a pre-mRNA influences a number of processing steps including alternative splicing. Since most splicing regulatory proteins bind to single-stranded RNA, the sequestration of RNA into double strands could prevent their binding. Here, we analyzed the secondary structure context of experimentally determined splicing enhancer and silencer motifs in their natural pre-mRNA context. We found that these splicing motifs are significantly more single-stranded than controls. These findings were validated by transfection experiments, where the effect of enhancer or silencer motifs on exon skipping was much more pronounced in single-stranded conformation. We also found that the structural context of predicted splicing motifs is under selection, suggesting a general importance of secondary structures on splicing and adding another level of evolutionary constraints on pre-mRNAs. Our results explain the action of mutations that affect splicing and indicate that the structural context of splicing motifs is part of the mRNA splicing code.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Hiller M,Zhang Z,Backofen R,Stamm Sdoi
10.1371/journal.pgen.0030204subject
Has Abstractpub_date
2007-11-01 00:00:00pages
e204issue
11eissn
1553-7390issn
1553-7404pii
07-PLGE-RA-0460journal_volume
3pub_type
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