The α1 subunit of nicotinic acetylcholine receptors in the inner ear: transcriptional regulation by ATOH1 and co-expression with the γ subunit in hair cells.

abstract：:There is much evidence to suggest that G protein coupled receptors exist as oligomers but the relevance to their function is unclear. We have, therefore, examined the binding of the radiolabelled agonist [(3)H]NPA to membranes of CHO cells expressing the D(2) dopamine receptor in dissociation rate experiments. When [(...

journal_title：Journal of neurochemistry

authors： Kara E,Lin H,Strange PG

更新日期：2010-03-01 00:00:00

abstract：:Preincubation of murine neuroblastoma cells (clone N1E-115) with terbium chloride resulted in a significant potentiation of carbachol-mediated increase in cyclic GMP formation. This effect was accompanied by a shift of the peak response from 30 s to 120 s and a 6-fold decrease in carbachol concentration producing half...

journal_title：Journal of neurochemistry

authors： El-Fakahany EE,Pfenning M,Richelson E

更新日期：1984-03-01 00:00:00

abstract：:GN11 and GT1-7 are immortalized gonadotropin-releasing hormone-positive murine cell lines exhibiting the features of immature olfactory neurons and differentiated hypothalamic neurons, respectively. Using electron microscopy and biochemical assays (RT-PCR and immunoblotting) we determined the presence of numerous cave...

journal_title：Journal of neurochemistry

authors： D'Orlando C,Guzzi F,Gravati M,Biella G,Toselli M,Meneveri R,Barisani D,Parenti M

更新日期：2008-03-01 00:00:00

abstract：:We investigated changes in the extracellular levels of acetylcholine (ACh) following local application of serotonergic agents to the dorsal hippocampus of freely moving rats by means of perfusion using a microdialysis technique. Perfusion of serotonin (5-HT; 10 microM, for 30 min at a rate of 3 microliters/min), disso...

journal_title：Journal of neurochemistry

authors： Izumi J,Washizuka M,Miura N,Hiraga Y,Ikeda Y

更新日期：1994-05-01 00:00:00

abstract：:Dibutyryl cyclic AMP (dBcAMP) is known to induce maturation and differentiation in astrocytes. As myo-inositol is an important osmoregulator in astrocytes, we examined the effects of maturation and biochemical differentiation on the kinetic properties of myo-inositol transport. Treatment of astrocytes with dBcAMP sign...

journal_title：Journal of neurochemistry

authors： Isaacks RE,Bender AS,Reuben JS,Kim CY,Shi YF,Norenberg MD

更新日期：1999-07-01 00:00:00

abstract：:This article is a critique of the paper by Khairnar et al. in the current issue of the Journal of Neurochemistry which describes the use of a sophisticated magnetic resonance imaging technique, diffusion kurtosis imaging, to investigate microstructural changes in the brain of TNWT-61 mice that are over-expressing α-sy...

journal_title：Journal of neurochemistry

authors： Williams S

更新日期：2016-03-01 00:00:00

abstract：:1-[2-(4-Aminophenyl)ethyl]-4-(3-trifluoromethylphenyl)piperazine (PAPP) inhibits [3H]5-hydroxytryptamine (5-HT, serotonin) binding to 5-HT1A and 5-HT1B sites in rat brain with apparent equilibrium dissociation constants (KD) of 2.9 and 328 nM, respectively. [3H]PAPP was synthesized, its binding to central serotonin re...

journal_title：Journal of neurochemistry

authors： Ransom RW,Asarch KB,Shih JC

更新日期：1986-01-01 00:00:00

abstract：:Neurons of the dorsal root ganglia (DRG) express a diversity of voltage-gated sodium channels. From rat DRG we have cloned and functionally expressed a tetrodotoxin-sensitive sodium channel alpha subunit, NaCh6/Scn8a/rPN4, and a splice variant, rPN4a. Primary structure analysis shows NaCh6/Scn8a/rPN4 to be highly homo...

journal_title：Journal of neurochemistry

authors： Dietrich PS,McGivern JG,Delgado SG,Koch BD,Eglen RM,Hunter JC,Sangameswaran L

更新日期：1998-06-01 00:00:00

abstract：:Brain-derived neurotrophic factor (BDNF) which is primarily associated with neuronal survivability, differentiation and synaptic plasticity has been reported to mediate neurodegeneration in hypoxia through its p75 Neurotrophin receptors (p75NTR). The molecular events promoting BDNF-mediated pro-death signalling in hyp...

journal_title：Journal of neurochemistry

authors： Sharma D,Barhwal KK,Biswal SN,Srivastava AK,Bhardwaj P,Kumar A,Chaurasia OP,Hota SK

更新日期：2019-01-01 00:00:00

abstract：:Hypoxic preconditioning (HP) 24 h before hypoxic-ischemic (HI) injury confers significant neuroprotection in neonatal rat brain. Recent studies have shown that the mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase (PI3K) intracellular signaling pathways play a role in the induction of tolerance...

journal_title：Journal of neurochemistry

authors： Jones NM,Bergeron M

更新日期：2004-04-01 00:00:00

abstract：:Local cerebral glucose utilization (LCGU) was measured, using the quantitative autoradiographic [14C]2-deoxy-D-glucose method, in 56 brain regions of 3-month-old, awake Fischer-344 rats, after intraperitoneal administration of sulpiride (SULP) 100 mg/kg. SULP, an "atypical" neuroleptic, is a selective antagonist of D2...

journal_title：Journal of neurochemistry

authors： Pizzolato G,Soncrant TT,Larson DM,Rapoport SI

更新日期：1987-08-01 00:00:00

abstract：:Hypoxia-inducible factor prolyl 4-hydroxylases (HIF-PHDs) are important targets against oxidative stress. We hypothesized that inhibition HIF-PHD by adaptaquin reduces hypoxic-ischemic brain injury in a neonatal mouse model. The pups were treated intraperitoneally immediately with adaptaquin after hypoxia-ischemia (HI...

journal_title：Journal of neurochemistry

authors： Li K,Li T,Wang Y,Xu Y,Zhang S,Culmsee C,Wang X,Zhu C

更新日期：2019-09-01 00:00:00

abstract：:Regulated intramembrane proteolysis (RIP) controls the communication between cells and the extracellular environment. RIP is essential in the nervous system, but also in other tissues. In the RIP process, a membrane protein typically undergoes two consecutive cleavages. The first one results in the shedding of its ect...

journal_title：Journal of neurochemistry

authors： Lichtenthaler SF,Haass C,Steiner H

更新日期：2011-06-01 00:00:00

abstract：:Three different transcripts of the homeodomain gene termed pituitary homeobox (Ptx) 2 (Pitx2/Brx/Rieg/Solurshin/Arp) were cloned from different species encoding proteins belonging to the paired-like family of homeodomain proteins. Ptx2a (324 amino acids), Ptx2b (271 amino acids), and Ptx2c (318 amino acids) share the ...

journal_title：Journal of neurochemistry

authors： Smidt MP,Cox JJ,van Schaick HS,Coolen M,Schepers J,van der Kleij AM,Burbach JP

更新日期：2000-11-01 00:00:00

abstract：:The antisense strategy was used to unravel the functional contribution of the mRNAs encoding dopamine (DA) receptors to the multiple transduction mechanisms operated by DA in rat pituitary cells. An antisense oligonucleotide was designed to recognize seven nucleotides upstream and 11 nucleotides downstream from the in...

journal_title：Journal of neurochemistry

authors： Valerio A,Alberici A,Tinti C,Spano P,Memo M

更新日期：1994-04-01 00:00:00

abstract：:Parkinson's disease is a common neurodegenerative disorder with a mainly sporadic aetiology, although a number of monogenic familiar forms are known. Most of the motor symptoms are due to selective depletion of dopaminergic, neuromelanin-containing neurones of the substantia nigra pars compacta. Neuromelanin is the da...

journal_title：Journal of neurochemistry

authors： Fasano M,Bergamasco B,Lopiano L

更新日期：2006-02-01 00:00:00

abstract：:The glycogen content of primary cultured astrocytes was approximately doubled by incubation with 1 mM L-glutamate or L-aspartate. Other amino acids and excitatory neurotransmitters were without effect. The increase in glycogen level was not blocked by the glutamate receptor antagonist kynurenic acid but was completely...

journal_title：Journal of neurochemistry

authors： Swanson RA,Yu AC,Chan PH,Sharp FR

更新日期：1990-02-01 00:00:00

abstract：:We have examined the ability of KW-6002, an adenosine A2a antagonist, to modulate the dyskinetic effects of L-DOPA in 6-hydroxydopamine-lesioned rats. In animals rendered dyskinetic by a previous course of L-DOPA treatment, KW-6002 did not elicit any abnormal involuntary movements on its own, but failed to reduce the ...

journal_title：Journal of neurochemistry

authors： Lundblad M,Vaudano E,Cenci MA

更新日期：2003-03-01 00:00:00

abstract：:Tryptophan hydroxylase (TPH) is the initial and rate-limiting enzyme in serotonin biosynthesis. The enzyme activity is dependent on molecular oxygen, a tetrahydropterin cosubstrate, and ferrous iron. The present study demonstrates that TPH is inhibited by a novel compound, p-ethynylphenylalanine (pEPA), produced by th...

journal_title：Journal of neurochemistry

authors： Stokes AH,Xu Y,Daunais JA,Tamir H,Gershon MD,Butkerait P,Kayser B,Altman J,Beck W,Vrana KE

更新日期：2000-05-01 00:00:00

abstract：:To better understand metabotropic/ionotropic integration in neurons we have examined the regulation of M1 muscarinic acetylcholine (mACh) receptor signalling in mature (> 14 days in vitro), synaptically-active hippocampal neurons in culture. Using a protocol where neurons are exposed to an EC(50) concentration of the ...

journal_title：Journal of neurochemistry

authors： Willets JM,Nelson CP,Nahorski SR,Challiss RA

更新日期：2007-12-01 00:00:00

abstract：:The aim of the present study was to gain a deeper insight into the cell signaling pathways involved in the neuroprotection/neurorescue activity of the major green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG). EGCG (1 micro m) caused an immediate (30 min) down-regulation (approximately 40%) of Bad protein level...

journal_title：Journal of neurochemistry

authors： Kalfon L,Youdim MB,Mandel SA

更新日期：2007-02-01 00:00:00

abstract：:We have used thapsigargin (TG), a specific, irreversible inhibitor of endoplasmic reticulum (ER) Ca(2+)-ATPases, and caffeine, an agonist of the ryanodine receptor, to study the effect of emptying of ER calcium stores on protein synthesis in neuronal cells. TG at 1 microM caused a permanent inhibition of protein synth...

journal_title：Journal of neurochemistry

authors： Paschen W,Doutheil J,Gissel C,Treiman M

更新日期：1996-10-01 00:00:00

abstract：:Increasing evidence suggests that the dopaminergic degeneration which characterizes Parkinson's disease starts in the striatal dopamine terminals and progresses retrogradely to the body of dopamine cells in the substantia nigra. The role of striatal astrocytes in the striatal initiation of the dopaminergic degeneratio...

journal_title：Journal of neurochemistry

authors： Morales I,Sanchez A,Rodriguez-Sabate C,Rodriguez M

更新日期：2016-10-01 00:00:00

abstract：:To examine whether the concentration gradient of glutamine (Gln) drives concentrative Na(+)-independent uptake of neutral amino acids (NAA) in mouse cerebral astrocytes, uptake was compared in "Gln-depleted" and "Gln-replete" cultures. Uptake (30 min in Na(+)-free buffer) of histidine, kynurenine, leucine, tyrosine, a...

journal_title：Journal of neurochemistry

authors： Brookes N

更新日期：1992-07-01 00:00:00

abstract：:Spiroxatrine has been reported to be a 5-HT1A serotonin receptor antagonist. Therefore [3H]spiroxatrine was synthesized and its 5-HT1A receptor binding properties in homogenates of rat hippocampal membranes were characterized with the expectation that it would be the first 5-HT1A antagonist radioligand. [3H]8-Hydroxyd...

journal_title：Journal of neurochemistry

authors： Herrick-Davis K,Titeler M

更新日期：1988-02-01 00:00:00

abstract：:The [14C]deoxyglucose [Sokoloff et al., J. Neurochem. 28, 897-916 (1977)] and [6-14C]glucose [Hawkins et al., Am. J. Physiol. 248, C170-C176 (1985)] quantitative autoradiographic methods were used to measure regional brain glucose utilization in awake rats. The spatial resolution and qualitative appearance of the auto...

journal_title：Journal of neurochemistry

authors： Collins RC,McCandless DW,Wagman IL

更新日期：1987-11-01 00:00:00

abstract：:At the premyelinating stage, the Schwann cells of peripheral nerves are able to recognize the axon, to arrange themselves along it in a nonoverlapping manner, and finally to establish a one-to-one cell-axon relationship. The mechanism that regulates these processes is not known in detail. We found the existence of a s...

journal_title：Journal of neurochemistry

authors： Nakao J,Shinoda J,Nakai Y,Murase S,Uyemura K

更新日期：1997-05-01 00:00:00

abstract：:We have found NAD to be rapidly degraded by extracellular enzymes present on intact rat brain synaptosomes. The enzyme involved had the specificity of an NADase cleaving the molecule at the nicotinamide-glycoside linkage and was inhibited by nicotinamide mononucleotide (NMN). This inhibitor did not displace specific b...

journal_title：Journal of neurochemistry

authors： Snell CR,Snell PH,Richards CD

更新日期：1984-12-01 00:00:00

abstract：:Synaptosomal-associated protein of 25 kDa (SNAP-25) is thought to play a key role in vesicle exocytosis and in the control of transmitter release. However, the precise mechanisms of action as well as the regulation of SNAP-25 remain unclear. Here we show by immunoprecipitation that activation of protein kinase C (PKC)...

journal_title：Journal of neurochemistry

authors： Genoud S,Pralong W,Riederer BM,Eder L,Catsicas S,Muller D

更新日期：1999-04-01 00:00:00

abstract：:Substrate-induced endocytic trafficking of dopamine transporter (DAT) has been observed, but little is known about the regulation of DAT oligomerization by substrate. The present study investigates the effect on substrates on DAT oligomerization and explores a potential link with the presence of DAT at the cell surfac...

journal_title：Journal of neurochemistry

authors： Chen N,Reith ME

更新日期：2008-05-01 00:00:00