Low levels of pyrophosphate in transgenic potato plants expressing E. coli pyrophosphatase lead to decreased vitality under oxygen deficiency.

Abstract:

BACKGROUND AND AIMS:The aim of this study was to investigate the importance of pyrophosphate (PPi) for plant metabolism and survival under low oxygen stress. Responses of roots of wild-type potato plants were compared with roots of transgenic plants containing decreased amounts of PPi as a result of the constitutive expression of Escherichia coli pyrophosphatase in the cytosol. METHODS:For the experiments, roots of young wild-type and transgenic potato plants growing in nutrient solution were flushed for 4 d with nitrogen, and subsequently metabolite contents as well as enzyme activities of the glycolytic pathway were determined. KEY RESULTS AND CONCLUSIONS:In roots of transgenic plants containing 40% less PPi, UDPglucose accumulated while the concentrations of hexose-6-phosphate, other glycolytic intermediates and ATP were decreased, leading to a growth retardation in aerated conditions. Apart from metabolic alterations, the activity of sucrose synthase was increased to a lower extent in the transgenic line than in wild type during hypoxia. These data suggest that sucrose cleavage was inhibited due to PPi deficiency already under aerated conditions, which has severe consequences for plant vitality under low oxygen. This is indicated by a reduction in the glycolytic activity, lower ATP levels and an impaired ability to resume growth after 4 d of hypoxia. Interestingly, the phosphorylation of fructose-6-phosphate via PPi-dependent phosphofructokinase was not altered in roots of transgenic plants. Nevertheless, our data provide some evidence for the importance of PPi to maintain plant growth and metabolism under oxygen deprivation.

journal_name

Ann Bot

journal_title

Annals of botany

authors

Mustroph A,Albrecht G,Hajirezaei M,Grimm B,Biemelt S

doi

10.1093/aob/mci223

keywords:

subject

Has Abstract

pub_date

2005-09-01 00:00:00

pages

717-26

issue

4

eissn

0305-7364

issn

1095-8290

pii

mci223

journal_volume

96

pub_type

杂志文章
  • Interactive effects of salinity and inundation on native Spartina foliosa, invasive S. densiflora and their hybrid from San Francisco Estuary, California.

    abstract:BACKGROUND AND AIMS:Sea level rise (SLR) associated with climate change is intensifying permanent submersion and salinity in salt marshes. In this scenario, hybridization between native and invasive species may result in hybrids having greater tolerance of abiotic stress factors than their parents. Thus, understanding ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcz170

    authors: Gallego-Tévar B,Grewell BJ,Futrell CJ,Drenovsky RE,Castillo JM

    更新日期:2020-02-03 00:00:00

  • Phylogeography of the tree Hymenaea stigonocarpa (Fabaceae: Caesalpinioideae) and the influence of quaternary climate changes in the Brazilian cerrado.

    abstract:BACKGROUND AND AIMS:Hymenaea stigonocarpa (Fabaceae: Caesalpinioideae) is an endemic tree from the Brazilian cerrado (savanna vegetation), a biome classified as a hotspot for conservation priority. This study investigates the phylogeographic structure of H. stigonocarpa, in order to understand the processes that have l...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcm221

    authors: Ramos AC,Lemos-Filho JP,Ribeiro RA,Santos FR,Lovato MB

    更新日期:2007-12-01 00:00:00

  • Physical constraints on temperature difference in some thermogenic aroid inflorescences.

    abstract:BACKGROUNDS AND AIMS:Thermogenesis in reproductive organs is known from several plant families, including the Araceae. A study was made of the relationship between temperature increase and spadix size in the subfamily Aroideae in order to determine whether the quantitative variation of heat production among species and...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mci157

    authors: Gibernau M,Barabé D,Moisson M,Trombe A

    更新日期:2005-07-01 00:00:00

  • Pushed to the limit: consequences of climate change for the Araucariaceae: a relictual rain forest family.

    abstract:BACKGROUND AND AIMS:Under predicted climate change scenarios, increased temperatures are likely to predispose trees to leaf and other tissue damage, resulting in plant death and contraction of already narrow distribution ranges in many relictual species. The effects of predicted upward temperatures may be further exace...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcr135

    authors: Offord CA

    更新日期:2011-08-01 00:00:00

  • Sensitivity of the invasive geophyte Oxalis pes-caprae to nutrient availability and competition.

    abstract:BACKGROUND AND AIMS:Invasion by alien plants may be partially related to disturbance-related increases in nutrient availability and decreases of competition with native species, and to superior competitive ability of the invader. Oxalis pes-caprae is an invasive winter geophyte in the Mediterranean Islands that reprodu...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcl289

    authors: Sala A,Verdaguer D,Vilà M

    更新日期:2007-04-01 00:00:00

  • International Code for Phytolith Nomenclature (ICPN) 2.0.

    abstract:BACKGROUND:Opal phytoliths (microscopic silica bodies produced in and between the cells of many plants) are a very resilient, often preserved type of plant microfossil. With the exponentially growing number of phytolith studies, standardization of phytolith morphotype names and description is essential. As a first effo...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcz064

    authors: International Committee for Phytolith Taxonomy (ICPT).

    更新日期:2019-09-24 00:00:00

  • Arachis batizocoi: a study of its relationship to cultivated peanut (A. hypogaea) and its potential for introgression of wild genes into the peanut crop using induced allotetraploids.

    abstract:BACKGROUND AND AIMS:Arachis batizocoi is a wild relative of cultivated peanut (A. hypogaea), an allotetraploid with an AABB genome. Arachis batizocoi was once considered the ancestral donor of the peanut B genome, but cytogenetics and DNA phylogenies have indicated a new genome classification, 'K'. These observations s...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcu237

    authors: Leal-Bertioli SC,Santos SP,Dantas KM,Inglis PW,Nielen S,Araujo AC,Silva JP,Cavalcante U,Guimarães PM,Brasileiro AC,Carrasquilla-Garcia N,Penmetsa RV,Cook D,Moretzsohn MC,Bertioli DJ

    更新日期:2015-02-01 00:00:00

  • Phytochrome B enhances plant growth, biomass and grain yield in field-grown maize.

    abstract:BACKGROUND AND AIMS:Phytochrome B (phyB) is a photosensory receptor important for the control of plant plasticity and resource partitioning. Whether phyB is required to optimize plant biomass accumulation in agricultural crops exposed to full sunlight is unknown. Here we investigated the impact of mutations in the gene...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcz015

    authors: Wies G,Mantese AI,Casal JJ,Maddonni GÁ

    更新日期:2019-06-24 00:00:00

  • Leaf cuticle analyses: implications for the existence of cutan/non-ester cutin and its biosynthetic origin.

    abstract:BACKGROUND AND AIMS:The cuticle of a limited number of plant species contains cutan, a chemically highly resistant biopolymer. As yet, the biosynthesis of cutan is not fully understood. Attempting to further unravel the origin of cutan, we analysed the chemical composition of enzymatically isolated cuticular membranes ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcaa056

    authors: Leide J,Nierop KGJ,Deininger AC,Staiger S,Riederer M,de Leeuw JW

    更新日期:2020-06-19 00:00:00

  • Plant material features responsible for bamboo's excellent mechanical performance: a comparison of tensile properties of bamboo and spruce at the tissue, fibre and cell wall levels.

    abstract:BACKGROUND AND AIMS:Bamboo is well known for its fast growth and excellent mechanical performance, but the underlying relationships between its structure and properties are only partially known. Since it lacks secondary thickening, bamboo cannot use adaptive growth in the same way as a tree would in order to modify the...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcu180

    authors: Wang X,Keplinger T,Gierlinger N,Burgert I

    更新日期:2014-12-01 00:00:00

  • Long-term water stress leads to acclimation of drought sensitivity of photosynthetic capacity in xeric but not riparian Eucalyptus species.

    abstract:BACKGROUND AND AIMS:Experimental drought is well documented to induce a decline in photosynthetic capacity. However, if given time to acclimate to low water availability, the photosynthetic responses of plants to low soil moisture content may differ from those found in short-term experiments. This study aims to test wh...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcv161

    authors: Zhou SX,Medlyn BE,Prentice IC

    更新日期:2016-01-01 00:00:00

  • Genome size diversity in orchids: consequences and evolution.

    abstract:BACKGROUND:The amount of DNA comprising the genome of an organism (its genome size) varies a remarkable 40 000-fold across eukaryotes, yet most groups are characterized by much narrower ranges (e.g. 14-fold in gymnosperms, 3- to 4-fold in mammals). Angiosperms stand out as one of the most variable groups with genome si...

    journal_title:Annals of botany

    pub_type: 杂志文章,评审

    doi:10.1093/aob/mcp003

    authors: Leitch IJ,Kahandawala I,Suda J,Hanson L,Ingrouille MJ,Chase MW,Fay MF

    更新日期:2009-08-01 00:00:00

  • How effectively does a clinostat mimic the ultrastructural effects of microgravity on plant cells?

    abstract::Columella cells of seedlings of Zea mays L. cv. Bear Hybrid grown in the microgravity of orbital flight allocate significantly larger relative-volumes to hyaloplasm and lipid bodies, and significantly smaller relative-volumes to dictyosomes, plastids, and starch than do columella cells of seedlings grown at 1 g. The...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.aob.a087926

    authors: Moore R

    更新日期:1990-01-01 00:00:00

  • Shifts in fine root traits within and among species along a fine-scale hydrological gradient.

    abstract:BACKGROUND AND AIMS:Lessons from above-ground trait ecology and resource economics theory may not be directly translatable to below-ground traits due to differences in function, trade-offs and environmental constraints. Here we examine root functional traits within and across species along a fine-scale hydrological gra...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcaa175

    authors: Taseski GM,Keith DA,Dalrymple RL,Cornwell WK

    更新日期:2020-09-23 00:00:00

  • Floral ontogeny in ficinia and isolepis (cyperaceae), with focus on the nature and origin of the gynophore.

    abstract:BACKGROUND AND AIMS:The generic delimitations of Ficinia and Isolepis, sister genera in the Cypereae, are blurred. Typical Ficinia flowers have a lobed gynophore, which envelops the base of the nutlet, whereas in Isolepis the character is considered to be absent. Some former species of Isolepis, lacking the gynophore, ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mci276

    authors: Vrijdaghs A,Goetghebeur P,Muasya AM,Caris P,Smets E

    更新日期:2005-12-01 00:00:00

  • Day-night changes of energy-rich compounds in crassulacean acid metabolism (CAM) species utilizing hexose and starch.

    abstract:BACKGROUND AND AIMS:Plants with crassulacean acid metabolism (CAM) can be divided into two groups according to the major carbohydrates used for malic acid synthesis, either polysaccharide (starch) or monosaccharide (hexose). This is related to the mechanism and affects energy metabolism in the two groups. In Kalanchoë ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mch165

    authors: Chen LS,Nose A

    更新日期:2004-09-01 00:00:00

  • Phenology of flowering and starch accumulation in grape (Vitis vinifera L.) cuttings and vines.

    abstract:BACKGROUND AND AIMS:A reliable protocol for flowering and fruiting in cuttings was developed with the aim of (a) studying inflorescence and flower development in grapevine cuttings and field plants, and (b) assisting haploid plant production. METHODS:Inflorescence and flower development was studied in 'Gewurztraminer'...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mci108

    authors: Lebon G,Duchêne E,Brun O,Clément C

    更新日期:2005-05-01 00:00:00

  • Are bryophytes shade plants? Photosynthetic light responses and proportions of chlorophyll a, chlorophyll b and total carotenoids.

    abstract:BACKGROUND AND AIMS:Data are presented from 39 species of mosses and 16 liverworts for ratios of chlorophylls and total carotenoids, and light saturation of photosynthetic electron flow or photosynthetic CO2 uptake, in relation to the postulate that bryophyte cells in general show shade-plant characteristics. METHODS:...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mch178

    authors: Marschall M,Proctor MC

    更新日期:2004-10-01 00:00:00

  • Dissecting the 'bacon and eggs' phenotype: transcriptomics of post-anthesis colour change in Lotus.

    abstract:Background and Aims:Post-anthesis colour change (PACC) is widely thought to be an adaptation to signal floral suitability to pollinators. Lotus filicaulis and Lotus sessilifolius are insect-pollinated herbaceous legumes with flowers that open yellow, shift to orange and finally red. This study examines the molecular ba...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcx090

    authors: Boehm MMA,Ojeda DI,Cronk QCB

    更新日期:2017-10-17 00:00:00

  • Sympodial construction of Fibonacci-type leaf rosettes in Pinguicula moranensis (Lentibulariaceae).

    abstract:BACKGROUND AND AIMS:The leaf rosettes of the carnivorous Pinguicula moranensis follow a spiral phyllotaxis approaching a Fibonacci pattern while the stalked flowers arise from extra-axillary sites between the leaves. The organization of this rosette has been discussed by various authors, with various results. The aim o...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcm184

    authors: Grob V,Pfeifer E,Rutishauser R

    更新日期:2007-10-01 00:00:00

  • The pollination of Trimenia moorei (Trimeniaceae): floral volatiles, insect/wind pollen vectors and stigmatic self-incompatibility in a basal angiosperm.

    abstract::Trimenia moorei (Oliv.) Philipson is an andromonoecious liane with >0.40 of the total flower buds maturing as bisexual flowers. Male and bisexual flowers are strongly scented with pollen, anther sacs and receptacle scars testing positively for volatile emissions. Scent analyses detect over 20 components. The major fat...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcg157

    authors: Bernhardt P,Sage T,Weston P,Azuma H,Lam M,Thien LB,Bruhl J

    更新日期:2003-09-01 00:00:00

  • Root signals and stomatal closure in relation to photosynthesis, chlorophyll a fluorescence and adventitious rooting of flooded tomato plants.

    abstract:BACKGROUND AND AIMS:An investigation was carried out to determine whether stomatal closure in flooded tomato plants (Solanum lycopersicum) results from decreased leaf water potentials (psi(L)), decreased photosynthetic capacity and attendant increases in internal CO(2) (C(i)) or from losses of root function such as cyt...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcn208

    authors: Else MA,Janowiak F,Atkinson CJ,Jackson MB

    更新日期:2009-01-01 00:00:00

  • GPT2: a glucose 6-phosphate/phosphate translocator with a novel role in the regulation of sugar signalling during seedling development.

    abstract:BACKGROUND AND AIMS:GPT2, a glucose 6-phosphate/phosphate translocator, plays an important role in environmental sensing in mature leaves of Arabidopsis thaliana. Its expression has also been detected in arabidopsis seeds and seedlings. In order to examine the role of this protein early in development, germination and ...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mct298

    authors: Dyson BC,Webster RE,Johnson GN

    更新日期:2014-03-01 00:00:00

  • The ups and downs of genome size evolution in polyploid species of Nicotiana (Solanaceae).

    abstract:BACKGROUND:In studies looking at individual polyploid species, the most common patterns of genomic change are that either genome size in the polyploid is additive (i.e. the sum of parental genome donors) or there is evidence of genome downsizing. Reports showing an increase in genome size are rare. In a large-scale ana...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcm326

    authors: Leitch IJ,Hanson L,Lim KY,Kovarik A,Chase MW,Clarkson JJ,Leitch AR

    更新日期:2008-04-01 00:00:00

  • Plant traits and ecosystem effects of clonality: a new research agenda.

    abstract:BACKGROUND:Clonal plants spread laterally by spacers between their ramets (shoot-root units); these spacers can transport and store resources. While much is known about how clonality promotes plant fitness, we know little about how different clonal plants influence ecosystem functions related to carbon, nutrient and wa...

    journal_title:Annals of botany

    pub_type: 杂志文章,评审

    doi:10.1093/aob/mcu113

    authors: Cornelissen JH,Song YB,Yu FH,Dong M

    更新日期:2014-08-01 00:00:00

  • The effects of large herbivores on the landscape dynamics of a perennial herb.

    abstract:BACKGROUND AND AIMS:Models assessing the prospects of plant species at the landscape level often focus primarily on the relationship between species dynamics and landscape structure. However, the short-term prospects of species with slow responses to landscape changes depend on the factors affecting local population dy...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcs064

    authors: Hemrová L,Cervenková Z,Münzbergová Z

    更新日期:2012-11-01 00:00:00

  • How many genes are needed to make a pollen tube? Lessons from transcriptomics.

    abstract:BACKGROUND:Pollen is the male gametophyte of higher plants. Upon pollination, it germinates and develops into a fast-growing cytoplasmic extension, the pollen tube, which ultimately delivers the sperm into the ovary. The biological relevance of its role, and the uniqueness of this kind of cellular organization, have ma...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcm208

    authors: Becker JD,Feijó JA

    更新日期:2007-12-01 00:00:00

  • The importance of barley genetics and domestication in a global perspective.

    abstract:BACKGROUND:Archaeological evidence has revealed that barley (Hordeum vulgare) is one of the oldest crops used by ancient farmers. Studies of the time and place of barley domestication may help in understanding ancient human civilization. SCOPE:The studies of domesticated genes in crops have uncovered the mechanisms wh...

    journal_title:Annals of botany

    pub_type: 历史文章,杂志文章,评审

    doi:10.1093/aob/mcm139

    authors: Pourkheirandish M,Komatsuda T

    更新日期:2007-11-01 00:00:00

  • Minimal temperature of pollen germination controls species distribution along a temperature gradient.

    abstract:BACKGROUND AND AIMS:Although plant distribution patterns are well documented, our understanding of the ecophysiological mechanisms that control the geographical ranges of plant species remains poor. We used a largely ignored method, the performance of the male gametophyte in vitro, to assess whether the thermal range o...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcw041

    authors: Rosbakh S,Poschlod P

    更新日期:2016-06-01 00:00:00

  • The effects of salinity and sodicity upon nodulation and nitrogen fixation in chickpea (Cicer arietinum).

    abstract::Production of grain legumes is severely reduced in salt-affected soils because their ability to form and maintain nitrogen-fixing nodules is impaired by both salinity and sodicity (alkalinity). Genotypes of chickpea, Cicer arietinum, with high nodulation capacity under stress were identified by field screening in a so...

    journal_title:Annals of botany

    pub_type: 杂志文章

    doi:10.1093/aob/mcf097

    authors: Rao DL,Giller KE,Yeo AR,Flowers TJ

    更新日期:2002-05-01 00:00:00